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Microwaving mycellum
    #6697650 -

So I have a problem - I've lost all my shroom jars except 2 to bacteria. I was growing the jars from mycellum, and I think the bacteria came with it.

Anyway, I was wondering if you can microwave mycellum that are tenuously hanging on? I would like to kill the bacteria, and leave the fungi.

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Re: Microwaving mycellum [Re: acoustick1tty]
    #6697671 -

Lol. You definitely don't want to do that! High heat exposure will simply give your even more contamination.

What color is the contam?

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Re: Microwaving mycellum [Re: Feanor]
    #6697697 -

It's not a color, not a fungus. What happens is that nothing will grow, the substrait will look slightly digested or broken down, and when opened there is a stench of unbelievable proportunes.

I saw in another post that shrooms can live in up to 130 degrees or so...

---

This also sort of lead me to wonder whether theres any chance I could get mutant fungus from doing this? Failing that, has anyone attempted to induce mutations by putting small amounts of radioactive material in the cakes? I read (possibly incorrectly) that theres radioactive stuff in fire alarms - does anyone know what type of radioactivity that is (beta / gamma)?

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Re: Microwaving mycellum [Re: acoustick1tty]
    #6697905 -

Microwave radiation will heat all water and fats to boiling - and the mycellium has a lot of water inside, and is surrounded by fluids. You will kill it in a microwave and there is no doubt about it.
What you describe is a totally lost cause :frown: There is no way you can save a jar with bacterial contamination which already smells bad. If it is a very, very small spot and the mycellium has colonized... say... 98%, then there is a chance that it may cover the spot, but still this usually does not happen.

Next time make your substrate totally dry to the touch before PC-ing. If you use grains for your jars follow the small tek in my signature and the moisture content will be perfect.

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Re: Microwaving mycellum [Re: acoustick1tty]
    #6697932 -

Quote:
Originally posted by acoustick1tty

...the substrait will look slightly digested or broken down, and when opened there is a stench of unbelievable proportunes.



Sounds like Wet Spot; Sour Rot - Bacillus to me.

Quote:
Originally found here.

In grain spawn jars, one commonly encounters Bacillus, which sometimes survives the sterilization process as heat resistant endospores. . A dull gray to mucus-like brownish slime characterized by a strong but foul odor variously described as smelling like rotting apples, dirty socks or burnt bacon. Bacillus makes uncolonized grain appear excessively wet, hence the name "Wet Spot". Pallid to whitish ridges along the margins of individual grain kernels characterize this contaminant. Bacillus primarily reproduces through simple cell division. In times of adverse environmental conditions, especially heat, a single hardened spore forms within each parent cell body - bacterial endospores, which can survive high temperatures for prolonged time.
The most practical method for eliminating bacterial endospores involves soaking the grain at room temperature 12 - 24 hours prior to sterilization. Endospores, if viable, will germinate within that time frame and then be susceptible to standard sterilization procedures. And, new endospores won't form in the moist environment of the resting jar of grain.



-Hoots


--------------------
I am, but an owl, in the sky.

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Re: Microwaving mycellum [Re: the_owl]
    #6697951 -

Bacillus Is terrible,once you have it your screwed there is no way to save it. Even if you could kill it in the microwave you would kill everything in the jar including your mycelium

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Re: Microwaving mycellum [Re: Blutjager]
    #6697982 -

Quote:
Originally posted by Blutjager

Bacillus Is terrible,once you have it your screwed there is no way to save it.



I totally agree. I would ditch the contaminated item(s), give the jar(s) a good wash/scrub down and start over.

-Hoots


--------------------
I am, but an owl, in the sky.

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Re: Microwaving mycellum [Re: the_owl]
    #6698677 -

Your microwave can take out the bacteria, and even most of the endospores of the bacteria, but not before it turns the mycelium into a lifeless chunk of carbon.

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