Home | Community | Message Board


Zamnesia.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: 10cc Syringe, Agar, Petri Dish, pH Test Strips

Jump to first unread post. Pages: 1
Offlinefelixhigh
KIA
Male User Gallery

Registered: 06/24/01
Posts: 7,543
Loc: Ly
Last seen: 3 years, 8 months
eritromicine in agar medium
    #474081 - 11/29/01 10:36 PM (15 years, 3 months ago)

hi folks! my wookie friend has a question... he wants to know if its possible to use eritromicine in agar mediums and if yes he wants to know if its ok to use with spores, unlike h2o2... thanks everybody.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblealtarego
member
Registered: 10/25/01
Posts: 130
Re: eritromicine in agar medium [Re: felixhigh]
    #474551 - 11/30/01 10:58 AM (15 years, 2 months ago)

As far as I know erythromicin does not survive autoclaving, which is why the antibiotic of choice is gentamicin sulfate (which does).

And yes, spores will germinate with antibiotics, which only kill bacteria.

- AE


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAzure
old hand
Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 14 years, 9 months
Re: eritromicine in agar medium [Re: altarego]
    #474989 - 11/30/01 05:30 PM (15 years, 2 months ago)

What sort of chemical stimulates germination of spores? I had a fresh spore print of Agaricus Augustus and I innoculated several petri dishes with spores. It's been almost three months and still no germination (nor contamination). Spores of other species on the same exact medium germinated just fine.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineWorkmanV
Psilocybe Microscopist
Male User Gallery

Registered: 03/01/01
Posts: 3,492
Loc: Washington, USA
Last seen: 2 years, 1 month
Trusted Cultivator
Re: eritromicine in agar medium [Re: Azure]
    #475016 - 11/30/01 05:52 PM (15 years, 2 months ago)

I know it can be done but I haven't been able to get clones or spores of Agaricus augustus to do anything at all. Try adjusting the pH.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefelixhigh
KIA
Male User Gallery

Registered: 06/24/01
Posts: 7,543
Loc: Ly
Last seen: 3 years, 8 months
Re: eritromicine in agar medium [Re: Azure]
    #475079 - 11/30/01 06:54 PM (15 years, 2 months ago)

i think you might need activated charcoal in order to germinate these spores


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepsilocybeen
Registered: 01/05/05
Posts: 58
Last seen: 4 years, 4 months
Re: eritromicine in agar medium [Re: felixhigh]
    #3701948 - 01/30/05 12:50 PM (12 years, 30 days ago)

I've encountered similar discrepancies with germination between species. I have been trying to germinate spores on plates containing a mix of ampicillin and kanamycin - and puffball spores germinated fine, while oysters and malabars didn't germinate at all. Weird


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: eritromicine in agar medium [Re: psilocybeen]
    #3702621 - 01/30/05 04:38 PM (12 years, 30 days ago)

well i cannot tell you if this is possible but i can quote something
anno once mentioned on the topic of antiobiotics.

he said not to use them unless you have to,
like saving a supa precious culture you only got one contammed plate
or something alike.

otherwise one shall never use antibiotics,
since if u happen to grow a contam, it will be resistant and your
going to be in some serious trouble.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleATWAR
Connoisseur

Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
Re: eritromicine in agar medium [Re: ohmatic]
    #3703730 - 01/30/05 08:49 PM (12 years, 30 days ago)

Quote:

ohmatic said:
since if u happen to grow a contam, it will be resistant and your
going to be in some serious trouble.




That is pretty much exactly the point.

Imagine growing out a pathogenic species that is also resistant. If you do not destroy the culture, you unleash it on the world (although other antibiotics may work, it doesn't matter - you have already made it resistant to one). I do not think we need to accelerate evolution of bacteria in this way. It is bad enough with all the antibacterial soaps, kitchen cleaners, and other things we use. These products have negative impacts by causing bacteria to become resistant to them, and by lowering our exposure the bacteria (thus limiting immune system stimuli). USE BLEACH TO CLEAN! Hot soapy water to wash with...


Use antibiotics only when you need them (contaminated spores would be the primary use, other isolation techniques usually work with live mycelium cultures). And if you use antibiotics and still grow a bacteria culture: Destroy the culture by autoclaving it, do not simply throw it away!


--------------------
To give is to live...



Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: eritromicine in agar medium [Re: ATWAR]
    #3706034 - 01/31/05 04:39 AM (12 years, 30 days ago)

jeppa thats it, autoklaver her at 18psi (or greater) for an hour and whats there shall be dead.

anyways, conecerning this fact, guides like http://www.kitchenculturekit.com act incredibly
irresponsible by stating:
Quote:


Allow media to cool until mixture is warm to touch, however, not solidified. Add 0.5 cc of Gentamicin Sulfate per 10 cc of solution (1.5 cc per cup)



(source http://www.kitchenculturekit.com/Mushroom/agarplates.htm)

especially since this guide aims at newbies, it will have already
caused a lot of them to breen mad contams flying around out there for sure.

as stated above, use antibiotics only in EMERGENCIES and never when its
not absolutely needed.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleCorporal Kielbasa
 User Gallery

Registered: 05/29/04
Posts: 17,029
Re: eritromicine in agar medium [Re: felixhigh]
    #3730164 - 02/04/05 02:27 PM (12 years, 25 days ago)

I should do some tests using powdered sulur in the agar. Its kills lots of things. I wonder if the mushroom can handle it.


--------------------




Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCatalysis
EtherealEngineer

Registered: 04/23/02
Posts: 1,742
Last seen: 8 years, 7 months
Re: eritromicine in agar medium [Re: felixhigh]
    #3732054 - 02/04/05 09:06 PM (12 years, 25 days ago)

Antibiotics are good for things like slants and long-term culture projects (i.e. strain isolation). They will not hurt anything but there is no reason to use them for short-term projects.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: 10cc Syringe, Agar, Petri Dish, pH Test Strips

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* readymade agar medium? felixhigh 835 2 11/16/01 06:28 PM
by felixhigh
* Agar Medium EvilGir 1,094 4 01/31/02 10:21 PM
by JuSMe13
* Agar medium with honey AnnoA 911 2 05/24/02 08:44 PM
by Zen Peddler
* Some proven agar nutrient media recipes Una 5,015 11 10/03/04 03:15 PM
by YSr
* Agar before PC'ing ABC 2,221 19 03/17/09 09:48 PM
by emstik5
* Interesting agar recipe (post corrected) MonkeyPod 2,408 10 04/18/05 01:18 PM
by MonkeyPod
* Growth of edible and medicinal mushrooms on commercial agar AnnoA 1,780 7 01/06/12 01:35 AM
by shantelleyu
* bohemica pinheads on agar Una 4,158 15 06/18/01 07:55 PM
by amanita

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
2,321 topic views. 0 members, 4 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
FreeSpores.com
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.002 seconds on 14 queries.