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OfflineMonkeyPod
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Registered: 04/02/05
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Last seen: 11 years, 4 months
Interesting agar recipe (post corrected)
    #4051118 - 04/13/05 05:29 PM (11 years, 7 months ago)

I happened upon this while googleing. It seems like a good recipe for mushrooms. I've never used agar medium but I'm considering. Any thoughts on this recipe? I did a search on shroomery and YPD didn't come up.

http://devinelab.biochem.emory.edu/YPD.html

Taken from link above:

Preparation of YPD Medium.

2X YPD Medium (1L):

Place in a 2L Erlenmyer flask:

20 g Bacto Yeast Extract (Difco #0127-17-9)
40 g Bacto Peptone (Difco #0118-17)
40 g Dextrose (Difco #215510)
0.6 g L-Tryptophan (Sigma #T-0254)
Water to 1 Liter (use water from the Millipore Milli Q machine)

Add a stir bar and stir until in solution
Autoclave 30 minutes on liquid cycle in a shallow tray of water


4% Agar

Place in a 2L Erlenmyer flask:

40 g Difco Agar (Difco #214010)
Water to 1 Liter (Milli Q water)

Autoclave 30 minutes on liquid cycle in a shallow tray of water

After removing from autoclave, gently swirl the flask to make sure the agar is completely suspended, and place on a hotplate until used.


Notes for preparing media:

After removing from the autoclave, add the following to the 2X YPD and stir:

1. For liquid medium: add sterile water to 2L (ready to use)

2. For plates, gently pour the 1L of 4% molten agar into the flask with the 2X YPD medium. Gently stir on a hotplate until the media is mixed well (avoid causing bubbles). Pour 30-40 mls into a standard 100mm plate.


Edited by MonkeyPod (04/13/05 09:35 PM)


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Offlinescooterpop
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Registered: 01/08/05
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Re: Interesting agar recipe [Re: MonkeyPod]
    #4051967 - 04/13/05 09:23 PM (11 years, 7 months ago)

link not working??


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OfflineMonkeyPod
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Re: Interesting agar recipe [Re: scooterpop]
    #4052002 - 04/13/05 09:32 PM (11 years, 7 months ago)

Sorry. The link is correct and worked earlier. Oh well. Anyway, I listed the recipe above.


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OfflineAeolus1369
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Re: Interesting agar recipe (post corrected) [Re: MonkeyPod]
    #4054998 - 04/14/05 03:45 PM (11 years, 7 months ago)

Looks good, but why go through all the trouble when other much simpler recipes work?  Ok, so maybe you get slightly faster growth...but that's probably not worth the added expense. :shrug:


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OfflineMonkeyPod
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Re: Interesting agar recipe (post corrected) [Re: Aeolus1369]
    #4057147 - 04/15/05 01:44 AM (11 years, 7 months ago)

I don't know really. I would think that if the mycelium gets off to a really good, healthy, start and it gets all of what it needs then it might be more inclined to fruit faster, produce stronger, more potent fruits and possibly be more contam resistant. As I said, I don't know...Why is there more than one agar recipe to begin with? I would venture to guess that some agars are more well suited for certain cultures.


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Offlinescatmanrav
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Re: Interesting agar recipe (post corrected) [Re: MonkeyPod]
    #4058019 - 04/15/05 10:38 AM (11 years, 7 months ago)

Well for one, its benificial to change nutrients if working with the same culture over and over again. Keeps the mycelium growing more livly then sticking with the same recipe over and over again.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineAeolus1369
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Re: Interesting agar recipe (post corrected) [Re: MonkeyPod]
    #4058116 - 04/15/05 11:19 AM (11 years, 7 months ago)

Quote:

MonkeyPod said:
I don't know really. I would think that if the mycelium gets off to a really good, healthy, start and it gets all of what it needs then it might be more inclined to fruit faster, produce stronger, more potent fruits and possibly be more contam resistant.




Ya, I'm just speculating as well. My thought would be any benefits imparted by the agar recipe would be insignificant once the mycelia was transferred to a different substrate since once colonized, the percentage of mycelium that originally grew on the enriched agar would be very small. Only one way to know for sure though...


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InvisibleArsey
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Re: Interesting agar recipe (post corrected) [Re: MonkeyPod]
    #4058446 - 04/15/05 12:59 PM (11 years, 7 months ago)

That's and interesting formula.
Personally I think it might not be so well suited for Ps. cubensis and the like.
The nitrogeneous components are well in excess of what you find in the commonly employed medias for these types of cultures.
The amount of yeast is roughly 10-20 times the norm, as is the peptone. Essenetial you'd be supplying 20 to 40 times the nitrogen, when compared to a MEYA or PDYA @ 1-2g peptone or yeast ext. In addition you'll wind up with a very high level of b vitamins from that amount of yeast.

In general mushrooms usual thrive on substrates with a high carbon to nitrogen ratio, it may be better if your nutrient media reflects that trend.


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OfflineMonkeyPod
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Re: Interesting agar recipe (post corrected) [Re: Arsey]
    #4061095 - 04/16/05 01:24 AM (11 years, 7 months ago)

So, are you saying to lower the peptone and yeast and add more dextrose? Is there something you would recommend adding to make the recipe more ideal?

I found a couple agar mediums on-line that were specific for fungi and they contained primarily soy peptone and dextrose (corn sugar). Would it be possible to boil soy beans (edamame) and corn and use the starch water for agar medium? Could this be useful?

Also, what about sweet potatoes or purple potatoes instead of regular potatoes for agar medium? Has anyone tried different kinds of potatoes?


Edited by MonkeyPod (04/16/05 01:30 AM)


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InvisibleArsey
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Re: Interesting agar recipe (post corrected) [Re: MonkeyPod]
    #4068203 - 04/18/05 11:38 AM (11 years, 7 months ago)

Quote:

MonkeyPod said:
So, are you saying to lower the peptone and yeast and add more dextrose? Is there something you would recommend adding to make the recipe more ideal?

I found a couple agar mediums on-line that were specific for fungi and they contained primarily soy peptone and dextrose (corn sugar). Would it be possible to boil soy beans (edamame) and corn and use the starch water for agar medium? Could this be useful?

Also, what about sweet potatoes or purple potatoes instead of regular potatoes for agar medium? Has anyone tried different kinds of potatoes?




I'm saying you don't need to use more that 1-2g of yeast extract or 1-2g of peptone per liter of media for mushrooms. Likewise, I think 40g of dextrose is okay however, 20g of dextrose with a potato infusion would likely perform better.

I don't knwo about the different potato colors although plain old brown baking potatos perform rather well.


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OfflineMonkeyPod
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Registered: 04/02/05
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Last seen: 11 years, 4 months
Re: Interesting agar recipe (post corrected) [Re: Arsey]
    #4068402 - 04/18/05 01:18 PM (11 years, 7 months ago)

Cool, thanks!
Other potato colors are usually much higher in antioxidants, esp. purple potatoes. The regular potato is part of the nightshade family. The sweet potato (yam) contains more sugars, antioxidants and such and is part of the morning glory family. I'm not sure if they would be more useful though.


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