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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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ag-grow-vation
    #435343 -

ok, ive finally got 2 jars looking somewhat good after just a few days if innoc. but all my others that ive ever done never colonize. im going on about 50+ jar attempts and i cant seem to get it. however if i dump my substrate jars (uncolonized) into a pan and case and saran wrap the top, they colonize butu i still can never get them to fruit (only done this 3 times and the first one i could not get to fruit, the other 2 are colonizing well right now and [hopefully] will fruit) when i case i put about 1/8 to 1/4" perlite then the uncolonized jars filling about 1.5-2" with substrate with no additional innoculation other than waht was originnaly in the jars. i am getting mycellium within a week or so. im keeping the casings in the same 'incubation chamber' as the original jars. its at 85 degrees, completely dark but not sealed. not a clue on the humidity (maybe 60%?) but again the casings are sealed. anyone ever hear of an issue like this?

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Re: ag-grow-vation [Re: BeppoMarx]
    #435366 -

You need to case fully colonized jars!

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Re: ag-grow-vation [Re: puscle]
    #435392 -

yeah i know. i cannot get a jar to colonize. i have a total of 24 jars, 12 half quart and 12 quart. ive been trying for upwards of 4 month sto get a jar to colonize. i cannot get that far. if i take an uncolonized jar after its sat for 2 weeks to a month and dump it in a tray and case it, it grows. thats my dilema.

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Re: ag-grow-vation [Re: BeppoMarx]
    #435398 -

and for teh record ive tried each jar thru 2 cycles, total of 50+ attempts and cant get one to go. the best ive seen is quarter sized mycellium in quart jars that halted after about 25 days (acadian coast cubensis) and that was in 5 of my first 12 jars i ever started and have not sen that kind of growth in jars since. about a month ago i switched to MMFD and still nada. ried many substrates also. the only good thing ive seen thus far is that ive lost only a few (7 or so) to contams on my first attempt but nothing since that first batch.

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Re: ag-grow-vation [Re: BeppoMarx]
    #435403 -

I want you to succeed, so consider this, 1/2 pints, PF-Tek and fresh syringes. You're gonna have to change directions, one way or the other.

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Re: ag-grow-vation [Re: puscle]
    #435486 -

sry. i meant 1/2 pint jars and quart jars. i guess ill read up on teh pf tek but ive read all teh teks here at shroomery and my first attemt was exactly according to MMGG 3.2 right down to 1/4 cup BRF, 1/4 cup water and 2/3 cup verm (which proved to be WAY too dry after dissecting the stunted jars). ive tried sporework's syringes and syringes made from sporeworks prints and ralphsters prints. puscle; as long as i have your attention- im trying cubensis (acadian and puerto rican) lipa yai, and pan cyans. i wanna know what you personally recommend and/or use for cultivating cubensis and if you have done puerto ricans did you change teh substrate at all? ive tried BRF/verm, whole grain brown rice, finch seed, and straw. i get quite frustrated at my cultivation attempts as i cannot find why i cant get mycellium to form inside the jars. im willing to try any 'tek' presented to me and im not gonna say that ive read them all by any means but ive read a whole lot of them and cant find why im still at ground zero.

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Re: ag-grow-vation [Re: BeppoMarx]
    #435499 -

you should probably switch to a a more arrgesive specis anyway, but for the jars you have now, when they are around half colonized of if the stall you should loosen the lids and flip them over for better air exchange, that could help.
I dont know wht else could be the problem, i dont know with out looking at the jars be maybe they are too wet and get bacteria which you cant allways see, or too dry, and run out of moisture.
I think some one else could gve you a better idea of whats going on.

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Re: ag-grow-vation [Re: BeppoMarx]
    #435517 -

Shake your jars when you see the first signs of good strong growth starting to slow down.
Use 1/3 cup water to 2/3 verm 1/4 BRF.


--------------------

"Puny earthlings do not yet know the meaning of the word suffering!!"


Edited by Necron99 (10/24/01 03:42 PM)

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Re: ag-grow-vation [Re: BeppoMarx]
    #435518 -

OK, 1 part home ground brown rice , 2 parts verm, 1 part water. Mix the verm and water first, then the rice. Pressure cook 1/2 pints for 30 minutes at 15 psi. I've never grown pan cyans. I like ALOT of different strains of cubensis. P.E.S.A., Equadors, B +, PF, Mazatapecs, Mexi-cubs, Aussies, Tazmanians and all of the strains from S. E. Asia, including the Lipa Yai . Shelve the whole grains and straw, at least for now.
What is (typically) stopping your jars from colonizing?

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Re: ag-grow-vation [Re: gusb232]
    #435526 -

'when they get half colonized' hah thats funny. a more aggressive species? like what? ill try one if you can recommend one. ive had jars that were too dry (when i opened the jar for inspection it broke off in chunks like play-doh and crumbled) and some that collected a small amount of water in the bottom. when they get too dry i notice that near the top (i use MM filter discs) that the substrate shrinks in diameter. in which case i add 5-10cc of sterile water via oven flow tek. all my jars smell either like the day they were prepared and some have a faint mushroom smell to them. i just feel like ive been playing on both sides of the spectrum for every issue that i (or others) can address. the only thing ive never checked is pH, as i dont have any litmus paper. i could get some but i didnt feel that it was necessary for this step. and after all my hard work on this i hear people like 'my mom cooked uncle bens rice a few weeks ago and i put some in a jar, nuked it and added spores and its colonized. now what?' thats not really relevant to our topic, it just irkes me, ya know? and please, any 'more aggressive' species are welcome. keep in mind that i have tried acadian coast cubensis, puertos, ps cyan (obviously outta my league) pan cyan, and lipa yai (thailand). i thought thai were aggressive colonizers but ive still had no luck.

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Re: ag-grow-vation [Re: BeppoMarx]
    #435533 -

Fuck the disks, they've only caused you failure. 1/2 inch dry verm barrier.

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Re: ag-grow-vation [Re: puscle]
    #435544 -

ok... when using flour (i have yet to grind the grains but i will try it) i have normally put verm in the mixing bowl, then flour and then water. im gonna abandon my older dried out PR jars and my dung/rye flour/verm jars and grind up some brown rice as per your recommendation. ive heard wild rice works as well if not better though for colonization and fruiting. any comments on this puscle? and as for 'What is (typically) stopping your jars from colonizing?'- i have no clue. spores almost always germinate and get faint lines (espeially around teh thistle seeds in the jars i have tried that in) but they just either sit there and look pretty or vanish after a week or so. ive found jars that are slightly too moist get this yellowish looking rhizomorphic formation in between the grains but it is more like excess water strung between the grains and stuck there. i believe its excess water that was colonized into mycellium water, but never grows outward at all. those jars smell mushroomy but its still mostly yellowish and not a pure white.

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Re: ag-grow-vation [Re: BeppoMarx]
    #435559 -

Wild rice is better? Shit, I'm gonna have to try it. This could revolutionize mycology! Where did you hear that?

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Re: ag-grow-vation [Re: puscle]
    #435602 -

ive only heard that here on the message boards that some people claim notice a potency increase when using wild rice. and i am not claiming i have tried it by any means just wanted to know if youve heard this. ive seen that posted more than once by different people. and as for the disks i have found them to dry out my jars a hella lot faster than the myc can run. my last batches that i stared this weekend i used regular 4 hole lids with discs on top of them instead of foil. do you think that this still would let too much moisture loss or would tinfoil be best over the lids? even so id prolly just use lids foil and discs mostly because its not the most sterile environment in my house. i mean its my parents house and its clean and all but i dont have a dedicated room for this or anything, theyre in my closet in a 4 shelf system which i hang my clothes in. my fruiting (i wish) chamber is sealed off by a 4 layer piece of saran wrap securely taped at the top with boxing tape and velcro on teh bottom so i can open it. its a metal cabinet and i use magnets on teh sides to keep it fairly sealed. my incubation shelf is the same xcept its a [clean] dishtowel (attempting to hold in heat and keep it dark) the whole thing is insulated with that blue styrofoam insulation that is used when siding houses on teh back and the top and sides are quilts folded to size and taped to the exterior. the inside is metal lined with shelving paper.

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Re: ag-grow-vation [Re: BeppoMarx]
    #435615 -

Oh, allright, I just figured wild rice might be too expensive.

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