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Amazon Shop: ½ Pint Jars, Agar, Petri Dish, Potato Dextrose, Pressure Cooker

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Offlinefiddler4u
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Registered: 08/20/01
Posts: 282
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Water on agar....
    #408185 - 09/29/01 12:22 PM (16 years, 1 month ago)

I made some agar, using the PDA tek. Everything looks good, except i see a small amount of water sitting on top of the agar after P.cooking. Is this a problem? How do I deal with it?
On another topic, I've put the jars of agar(1/2 pint jars), in some sandwich bags that I sprayed with bleach/water solution. Then wraped tight. There sitting in a closet as we speak. Any problems with this?
THX

I am the vine, you are the branches. If a man remains in me, and I in him, he will bare much fruit.
Apart from me you can do nothing. john 15:5


--------------------
I am the vine, you are the branches. If a man remains in me, and I in him, he will bare much fruit.
Apart from me you can do nothing. john 15:5


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OfflineDragonAlienFest1
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Re: Water on agar.... [Re: fiddler4u]
    #408398 - 09/29/01 05:35 PM (16 years, 1 month ago)

Did you cook the agar then pour it into the jars or did you pour, then cook?

You can let the agar cool and let more moisture evaporate before pouring into your jars if this applies. This should reduce the amount of condensation on the agar surface and the lid.

If you poured first, then cooked, allow the moisture to evaporate off of the agar by leaving the lid off, leaving the lid ajar or using filter discs.

You could also use less water in your recipe to reduce the amount of condensation.




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Completely straightup downright digging it. Can you dig it?


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Invisibleoscill8
*bondage fairy*
Registered: 02/27/01
Posts: 1,215
Re: Water on agar.... [Re: DragonAlienFest1]
    #408417 - 09/29/01 06:21 PM (16 years, 1 month ago)

Does anyone know the temp that agar should be poured at to eliminate condensation on the lids of petris, but where it is still *pourable*?



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? oscill8 2001, 2002
"Any data submitted to Shroomery.org becomes our property"


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Offlinefiddler4u
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Registered: 08/20/01
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Re: Water on agar.... [Re: DragonAlienFest1]
    #408679 - 09/30/01 12:35 AM (16 years, 1 month ago)

I cooked the agar, then I let it sit overnight(got alittle tired), then come morning, I cooked it again to liquify it(because it got hard by that time). After that I let the stuff cool alittle in the P.cooker, then pored the stuff in my jars. Then I put the jars in the P.cooker for 25min. Let everything cool. The rest you already know....
THX

I am the vine, you are the branches. If a man remains in me, and I in him, he will bare much fruit.
Apart from me you can do nothing. john 15:5


--------------------
I am the vine, you are the branches. If a man remains in me, and I in him, he will bare much fruit.
Apart from me you can do nothing. john 15:5


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OfflineElektrolurch
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Re: Water on agar.... [Re: oscill8]
    #408801 - 09/30/01 01:55 AM (16 years, 1 month ago)

Agar solidifies at 40 C to 45 C, depending on concentration. So pour it at 60C or lower, and you will prevent much of the condensation. Another way to prevent condensation is to stack the petris up, when letting them cool down. The upper ones will show condensation, but the lower ones not.

Elektrolurch

Edited by Elektrolurch on 09/30/01 12:56 AM.



--------------------
"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67


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Offlinefiddler4u
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Re: Water on agar.... [Re: Elektrolurch]
    #409219 - 09/30/01 02:59 PM (16 years, 1 month ago)

THX for the help....
Now I know the solution is under my nose, but I just can't see it. I now when the situation is bad you look for the stupid things...but I can't figure it out.....Why do the lower one show no signs of water?
This is buggin me...
THX again :)

I am the vine, you are the branches. If a man remains in me, and I in him, he will bare much fruit.
Apart from me you can do nothing. john 15:5


--------------------
I am the vine, you are the branches. If a man remains in me, and I in him, he will bare much fruit.
Apart from me you can do nothing. john 15:5


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Offlinepj541
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Registered: 02/04/01
Posts: 128
Loc: fuck off
Last seen: 15 years, 6 months
Re: Water on agar.... [Re: fiddler4u]
    #409229 - 09/30/01 03:27 PM (16 years, 1 month ago)

the condensation does not form on the lower dishes because there is little difference in temperature.
pj

The Shroomery needs help, ask Thor what you can do! This is your site too!


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?Well, this is how the world works. All energies flow according to the whims of the great magnet. What a fool I was to defy him.?



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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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