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 mahookah
journeyman
Registered: 08/07/01
Posts: 74
Loc: NV
Last seen: 21 years, 10 months
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 Please help (mycellium innoc. ques.) 
#396571 - 09/17/01 09:04 AM (22 years, 21 days ago) |
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I have just discovered that the reason that my foaf's cakes (15 1pt. of various types) have been so slow to colonize is because they all have what I guess is green mold. Needless to say WHAT THE FUCK! No Idea where this is coming from, it appears as a bright greenish yellow tint around the edges of the mycellium after about a week or so's growth. Anyway, he does have a couple of healthy verm casings and wants to use the mycellium from these to colonize some rye. Going to buy a pc today and would like to know what the best, most sterile method for mass colonization of substrate from a casing is. I'm trying to keep this short so I'll stop here and wait for any questions. Thanx and God bless America.
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bluhoney
Yes Im a realgirl ,geeesh
 
Registered: 05/23/99
Posts: 936
Last seen: 16 years, 7 months
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 Re: Please help (mycellium innoc. ques.) [Re: mahookah]
#396579 - 09/17/01 09:20 AM (22 years, 21 days ago) |
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The fastest and easiest is to use wheat straw. You need to first pasturize it. Do this by getting a large pot and a pillow case. Take as large amount of straw as you can to fill up the pillow case then put it in the pot. Fill it up with water until the pillow case is submerged then but some type of weight on top of it to keep it under water. Heat until almost boiling for about an hour. Drain and let cool. Take a pair of scissors and cut the wheat straw into small links and sprinkle these on top of a container filled with a small layer of vermiculite on the bottom and a good even thick ( 2 inches works fine) of chopped up colonized cake. Make the straw layer about an inch or so thick. Then place a piece of plastic tarp, or syran wrap over the "sandwich" and press down to compact the layers. Leave the plastic covering on and with a fork heated, poke alot of small holes into the plastic to let air in. Place in a dark clean enviroment for about a week. When you start to see white breaking through to the top layer of straw, take the plastic covering off and place in your growing set-up. A perlite set-up works fine in this situation.  bluhoney
 information posted is for entertainment purposes only and should not be attempted in real life
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Information listed here is for entertainment only and is neither real or proven
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mahookah
journeyman
Registered: 08/07/01
Posts: 74
Loc: NV
Last seen: 21 years, 10 months
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Re: Please help (mycellium innoc. ques.) [Re: bluhoney]
#396596 - 09/17/01 09:44 AM (22 years, 21 days ago) |
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Thanx for the help bluhoney, but the problem is I don't have any cakes left! they're all contamed. All I have is a couple of healthy cases (verm) that have already flushed once or twice that I want to use the mycellium (or maybe a chopped up healthy shroom) to innoculate lots of substrate. I was thinking I could break off a piece of my casing, wash it off, dunk it in a 10 to 1 h2o2 bath, and then put it in a sterile blender with some sterile water and make some mycelium syringes. I dont want to wait another 6 weeks for some spores to grow up and possibly get contaminated before I can even case them. Any thoughts? I'm reading the tek's in the grow section but any suggestions would be greatly appreciated. Also, do you think my mycellium syringe idea is ok?
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bluhoney
Yes Im a realgirl ,geeesh
Registered: 05/23/99
Posts: 936
Last seen: 16 years, 7 months
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Re: Please help (mycellium innoc. ques.) [Re: mahookah]
#396686 - 09/17/01 12:10 PM (22 years, 21 days ago) |
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Your syringe tek sounds fine. Opps on the misunderstanding of your post.LOL. I just reread it and I completely went out on that one.LOL, Make sure that your casing is completely germ free. And use the H2O2 to be safe. If you have a large pot, boil the blender minus the rubber gasket for about an hour to sterilize it. Or wash it out with H2O2 before blending. My friend has successfully used casing mycelia to make syringes which came out alright. You can as you stated as well take a small piece of a fully mature mushroom and place in a H2O2 solution and then innoculate or place the piece of mushroom on an agar dish and let it grow out to give you more culture to use. Let me know how it goes.
information posted is for entertainment purposes only and should not be attempted in real life
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Information listed here is for entertainment only and is neither real or proven
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mahookah
journeyman
Registered: 08/07/01
Posts: 74
Loc: NV
Last seen: 21 years, 10 months
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Re: Please help (mycellium innoc. ques.) [Re: bluhoney]
#396718 - 09/17/01 12:43 PM (22 years, 21 days ago) |
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Thanks buddy, I already tried taking a clump of my cake, washing it off, dipping in h2o2, and blend in sterile water, but it looked so scummy with all that verm and whateverthehellelse was in there. I decided to reclean my blender, some syringes, and boil up some substrate. Then I think I'm going to make my mycellium solution using fresh shrooms. Should be prettier. One question, can I dip a shroom in pure h2o2? If not, what ratio? Also how long can it take it for (dunking in h2o2). Thanks a million for your help, I just grew my first babies and I would like to keep it going w/o a six week lag yunnow?
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