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Offlinegodflesh
member

Registered: 07/13/01
Posts: 84
Last seen: 15 years, 4 months
Word to the Wise on MM Filter's
    #390142 - 09/10/01 12:38 PM (16 years, 19 days ago)

Just wanted to share my experience and a word of caution about Magic Micron Filter Disks. I have found that when using the PF method and the disks, it is VERY important that the disks not get wet during steralization. About 10 days ago I PC'd 12 of the Ball 1/2 pints (the short fat jars). In my PC I can put 7 on the bottom and 5 on top of them and get them all in at one time. After PC'ing them for 20 minutes at 17psi I noticed that I had put so much water in the bottom that it had bubbled up while boiling to get the filters on the bottom 7 wet. I noticed that some of the disks had water on top of the filters and others had just wicked water up from the sides of the disks. Anyway I figured there wasn't anything I could do now and innoculated anyway. I used a dry verm layer so I innoculated through the disk.

So I put them in a perlite humidified rubbermaid tub about 10 days ago. I just checked them this morning and 7 of the 12 jars were contaminated with green mold and one of the 7 had another orange and red contam in it. All of the contams where growing inbetween innoculation points so I knew it wasn't the needle or spores I used. I could see where the contams started on the disks and leeched their way along the disks then onto the verm (which got wet through the disks during PC'ing) and finally into the substrate. The five jars that were on top and didn't get wet are fine.

So anyway, Word to the Wise, don't get the disks wet or your screwed...

In addition I would like to say that colonization is very rapid with these disks. All of the jars are about 70-80% colonized after 10 days. It's just too bad I had to throw over half of them out...

Thanks for reading and I hope you learn from my mistake.



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OfflineCACA
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Registered: 07/12/01
Posts: 1,122
Last seen: 15 years, 5 months
Re: Word to the Wise on MM Filter's [Re: godflesh]
    #390147 - 09/10/01 12:52 PM (16 years, 19 days ago)

This information will be useful if I am ever lucky enough to get my hands on some of them MM disks. Thanks.

:frown: I believe in Mr. Grieves !


--------------------
"I am the vine, ye are the branches: He that abideth in me, and I in him, the same bringeth forth much fruit: for without me ye can do nothing." John 15:5


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Offlinepatzee
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Registered: 05/26/01
Posts: 233
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Re: Word to the Wise on MM Filter's [Re: godflesh]
    #390153 - 09/10/01 01:09 PM (16 years, 19 days ago)

double layer the disks? Let the first one dry if it gets wet..then put a new dry one on
top just after inoc. They're reusable, don't trim the edges.



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InvisibleNuShroomPharmerII
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Registered: 11/03/99
Posts: 453
Post deleted by users_request [Re: godflesh]
    #390200 - 09/10/01 02:20 PM (16 years, 19 days ago)



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Offlinegodflesh
member

Registered: 07/13/01
Posts: 84
Last seen: 15 years, 4 months
Re: Word to the Wise on MM Filter's [Re: NuShroomPharmerII]
    #390253 - 09/10/01 03:30 PM (16 years, 19 days ago)

In reply to:

Of course your screwed if the disc get too wet.




NSP, you say "Of course" but to a person who has never used the disks and is therefore ignorant of these matters there is no "Of course" anything. Thus the reason for my post, I'm trying to enlighten your future users of the disasterous results if the discs get wet.

I cut the disks just like the instructions on your site says. 1" around the tracing of the lid. This worked great and left very little of the filter exposed outside the lid.

I'm sure the verm layer did get wet and that's the main reason the contam was able to leech it's way through the filter...I will definately use aluminum foil on the next batch... Thanks for the advice.

Well I'm out, later.



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OfflineBBShrizz
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Registered: 03/01/01
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Re: Word to the Wise on MM Filter's [Re: godflesh]
    #390261 - 09/10/01 03:37 PM (16 years, 19 days ago)

a hair dryer may help if something *does* happen to make your MM disks wet.



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Invisiblestrang

Registered: 04/23/01
Posts: 671
Re: Word to the Wise on MM Filter's [Re: BBShrizz]
    #390279 - 09/10/01 03:56 PM (16 years, 19 days ago)

all of my discs have gotten wet during pc'ing even with foil on them and have never gotten contamination this way... i have done nearly 150 jars now with mm.... if you read the instructions on the mm site they say to use foil on top.. the little bit of moisture on the discs will usually dry by the tile your substate has cooled and is ready for innoculation.... i havent ever used two filter.. just tape the innoculation holes with a sliver of tape...



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Offlinejonnyshaggs420
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Registered: 08/09/00
Posts: 1,965
Loc: Mid-West
Last seen: 12 years, 1 day
Re: Word to the Wise on MM Filter's [Re: strang]
    #390323 - 09/10/01 04:36 PM (16 years, 19 days ago)

Ya, the disks that get wet I just let them dry out before I put them into the incubation chamber. I've had mold grow on the disks before when they were moist, so I just pat them dry when they first come out of the PC, and for those that are really wet I let them sit out in the open air for a day or so.

For supplies: Dr Bluethumb
For spores: FSR


--------------------
Vote Jonnyshaggs in the next election for GOD...Its the responsible choice


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InvisibleDazedSol
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Registered: 08/01/01
Posts: 1,230
Re: Word to the Wise on MM Filter's [Re: strang]
    #390328 - 09/10/01 04:40 PM (16 years, 19 days ago)

Yea my discs always get wet after PCing.....no matter what I try I can not keep them perfectly dry......I just wipe them off with a clean paper towel then let them cool down the rest of the way before innoculating......I havent had any major probs with contams because of this(only 4 out of 20 had green mold.)...... Ah well I think the filters work great.............

Adam,
-=Dazed Sol Productions=-


--------------------
Peace,
Adam


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InvisibleBillyblastoff
Psychoactivepredator
 User Gallery

Registered: 04/20/01
Posts: 725
Loc: Down these mean city stre...
Re: Word to the Wise on MM Filter's [Re: godflesh]
    #390378 - 09/10/01 05:36 PM (16 years, 19 days ago)

Ive just recently used NSP disks and I knew well before I used them to keep the disks as dry as possible and to take extra measures to dry em if they did get too wet.
When I sterilized my substrate jars I didnt have tin foil so I used sandwich bags around the jars and though this worked for the most part the disks did get alittle wet but nothing leaving em out in room temperature couldnt handle in a couple of hours. MY POINT BEING if you read alittle more of the threads around here concerning these disks you probably would've been much happier right now.
BTW most of my jars are contamed but I know what happened it had NOTHING to do with the Micron Magic THEY work great YOU SHOULD SEE HAS FAST THE CONTAMS ARE COLONIZING...LOL


Tell me something good then I might agree with you..


--------------------
"Imagination is more important than knowledge. For while knowledge defines all we currently know and understand, imagination points to all we might yet discover and create."

"There is a wealth of information built into us, tucked away in the genetic material in every one of our cells. The psychedelic drugs allow exploration of this interior world, and insights into its nature."

Tell me something good then I might agree with you....the Smithereens


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InvisibleLanaV
Head Banana
Female

Registered: 10/28/99
Posts: 3,088
Loc: www.MycoSupply.com
Re: Word to the Wise on MM Filter's [Re: Billyblastoff]
    #390391 - 09/10/01 05:57 PM (16 years, 19 days ago)

I understand both points that are brought up...

The novice beginner who may never have worked with filter discs before could run into these problems. And on the other hand, those who have researched them or have used them have better success rates. My point is, I wouldn't necessarily down the product. Myco Supply sells filter discs and any contamination that AFOAF has had wasn't caused by filter discs. Then again she's used them many times before.

Same thing can happen with spawn bags. If you fill your PC 1/4 of the way with water, your spawn will get soaked.

Please rememeber that a filter is porous, and when there are high air/steam pressures included, things (filters) may not hold up so well.

AND...one last thing. Filter discs are meant to protect against airborne contaminates. Not liquids:)

Lana

Distributors of Mycological Products

http://www.MycoSupply.com




--------------------
Myco Supply - Distributors of Mycological Products
http://www.MycoSupply.com

The Premiere Source for Mushroom Growing Supplies.
Visit us online or call us toll free


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