Home | Community | Message Board


Shroom Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon ½ Pint Jars, Agar, Brown Rice Flour, pH Test Strips, Potato Dextrose

Jump to first unread post. Pages: 1
OfflineJammer
Computers areMORE Addictive!

Registered: 11/05/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 11 years, 11 months
Mycelium Syringes and the PDA Tech.
    #387394 - 09/06/01 10:55 PM (16 years, 17 days ago)

Ok here is the situation: A well known vender's lab has just anounced that they will only replace containamanted syringes when they are tried with the PDA (potatoe,Dextrose,Agar) tech. The PDA tech. appears to be basicly growing the mycelium on top of, what I like to call, potatoe soup 1/2 filled in 1/2 pint jars. The plans conclude that by useing there mycelium suspension syringes with the PDA tech. that up to 4 oz's of mycelium can be produced that is the same strength as 'shrooms. Perhaps it is, but is this method any superior to say the PF TEK? The inplication is that the brown rice method some how promotes containamation. Is there any reason that a mycelium suspension syringe would not work with the PF TEK method? We suspect that it's a distraction from a badly containamated batch or so of syringes. If anyone has any expeirence with mycelium suspension syringes (they are legal- they are barely more than geramanated spores that are a few days old from what I gather) and/or the PDA tech. please reply to this post. It is VERY dificult to find any information on either of these subjects. Thanks.

Jammer



--------------------
>>Jammer>>


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 2 months, 23 days
Re: Mycelium Syringes and the PDA Tech. [Re: Jammer]
    #387680 - 09/07/01 04:10 AM (16 years, 17 days ago)

>Is there any reason that a mycelium suspension syringe
>would not work with the PF TEK method?
No, it works better and faster as the spore syringe.

-------------------
Visit www.fungifun.org formerly kickme.to/mushrooms

Be very, very careful what you put into that head,
because you will never, ever get it out.

Thomas Cardinal Wolsey (1471-1530)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinesylo
addict
Registered: 10/30/00
Posts: 219
Last seen: 15 years, 4 months
Re: Mycelium Syringes and the PDA Tech. [Re: Jammer]
    #387693 - 09/07/01 05:05 AM (16 years, 17 days ago)

I suspect you are right about the syringes being contaminated. PDA is a selective medium used to grow fungi. It has a high osmotic tension from the dextrose and a low pH. The sugar and acid prevent bacteria from growing but the concentrations are not so high that fungi, such as shrooms, will not grow. PDA is a good medium to use for growing mycelium from a print or syringe contaminated with bacteria but it is not the best medium for growing mycelium in quantities because the glucose and acid also reduce mycelial growth. It takes a lot of agar plates of any kind to grow a decent amount of mycelium otherwise this would be a good way to go.



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 2 months, 23 days
Re: Mycelium Syringes and the PDA Tech. [Re: sylo]
    #387701 - 09/07/01 05:46 AM (16 years, 17 days ago)

> The sugar and acid prevent bacteria from growing but the
>concentrations are not so high that fungi, such as shrooms,
>will not grow.
Unfortunately bacteria also thrive on PDA(Potato Dextrose Agar).

-------------------
Visit www.fungifun.org formerly kickme.to/mushrooms

Be very, very careful what you put into that head,
because you will never, ever get it out.

Thomas Cardinal Wolsey (1471-1530)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJammer
Computers areMORE Addictive!

Registered: 11/05/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 11 years, 11 months
Re: Mycelium Syringes and the PDA Tech. [Re: Anno]
    #388467 - 09/08/01 02:51 AM (16 years, 16 days ago)

True, but bacteria isnt as big of a threat as the GREEN KILLER is! - hehe



--------------------
>>Jammer>>


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinesylo
addict
Registered: 10/30/00
Posts: 219
Last seen: 15 years, 4 months
Re: Mycelium Syringes and the PDA Tech. [Re: Anno]
    #388522 - 09/08/01 05:44 AM (16 years, 16 days ago)

Anno,
You are right. Bacteria can grow on PDA. I was thinking of another agar at the time. SDA "Sabauraud's" is the agar that grows fungi but not bacteria.



Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon ½ Pint Jars, Agar, Brown Rice Flour, pH Test Strips, Potato Dextrose

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* PDA method.. Gonna give it a try!!! djveiox 1,403 1 12/31/01 09:51 AM
by SumGuy
* Is psylocybe in mycelium?
( 1 2 all )
Only1AK 9,614 22 12/14/14 05:13 AM
by Astral Pain
* PDA help tryptonite 864 9 04/20/04 04:02 AM
by tryptonite
* Shelf Life of Agar"s mycelium laden syringes? River77 511 4 05/11/09 06:04 AM
by River77
* Is the speed at which mycelium grows linear?
( 1 2 3 all )
CaptainCrunch 5,132 55 05/17/05 04:19 PM
by Roadkill
* bulk grow in days, tech help anyone? Varus 636 1 09/10/07 10:46 AM
by Dr_T
* A playground for my mushroom mycelium Peterthinks 985 9 08/30/07 03:22 PM
by ThePyschonaut52
* PDA + H2o2 questions Jon 480 3 07/10/05 02:21 AM
by Jon

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, RogerRabbit, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
1,593 topic views. 25 members, 127 guests and 41 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
High Mountain Compost
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.005 seconds on 16 queries.