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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Oyster bucket prep, dirty tek fafo
    #28915654 -

First grow. I started with Black Pearl, and Yellow oyster LC. I inoculated 2 jars each of WBS. At about 50% colonized, I shook each jar. They all re-colonized but later started looking wet with new condensation inside. Maybe it's not going to work. If not, I can watch it fail in real time. I have plenty of LC left and made some agar plates to work with.



I spent a couple hours chopping wheat straw and pulling out seed heads. And have rinsed the straw.




I wasn't planning to use lime or wash with soap. Any advice there?

My buckets are 2gal. After pasteurizing and hanging to cool, I'd layer the grain with the damp straw.

Things I'll do differently next time:
-soak the grain for 12 hours
-PC my grain jars for longer

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Re: Oyster bucket prep, dirty tek fafo [Re: aShogunNamedMarcus]
    #28915749 -

Decided to add a couple drops dish soap and soak the straw

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Re: Oyster bucket prep, dirty tek fafo [Re: aShogunNamedMarcus] * 1
    #28915835 -

Grains look good but without pasteurizing the straw the chance of contam goes up.

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Re: Oyster bucket prep, dirty tek fafo [Re: deadmandave]
    #28915854 -

deadmandave said:
Grains look good but without pasteurizing the straw the chance of contam goes up.


I will pasteurize the straw after the soak.

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Re: Oyster bucket prep, dirty tek fafo [Re: deadmandave]
    #28915857 -

deadmandave said:
Grains look good but without pasteurizing the straw the chance of contam goes up.


Clarification, I'll hot soak in 160° water for the proper time.

Were you referring to lime pasteurization?

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Re: Oyster bucket prep, dirty tek fafo [Re: aShogunNamedMarcus]
    #28915860 -

I still have a chance to buy HL but would rather not.

I will if I have to.

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Re: Oyster bucket prep, dirty tek fafo [Re: aShogunNamedMarcus]
    #28915921 -

Sounds like you've got the process down. 160F is the bare minimum for pasteurization, too. Let it cool, mix with spawn, and wait.

Were you looking to go for a lime soak instead?


--------------------
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"The world was made for the dead."
-Flannery O'Connor, "The Violent Bear It Away"

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Re: Oyster bucket prep, dirty tek fafo [Re: EdgarPoe]
    #28915938 -

EdgarPoe said:
Sounds like you've got the process down. 160F is the bare minimum for pasteurization, too. Let it cool, mix with spawn, and wait.

Were you looking to go for a lime soak instead?


I was trying to avoid the lime soak.

I have 2 large pressure cookers that I want to use to hold the temp at 160F.

Now, you say 160F is the minimum... everything I've read all day says 140-160 to pasteurize. I'll have to look again for how long.

I had planned to heat my water to 180° (simmer) then dump in straw which lowers the temp... then hold that temp.

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Re: Oyster bucket prep, dirty tek fafo [Re: aShogunNamedMarcus] * 1
    #28915949 -

Ah, ok. I must've misunderstood you on the lime.

Pasteurization at 140 seems doable. I've never done it at that temp, nor am I an expert at the process. I just do what works. I would recommend bringing the water to boil before adding the straw and allow the temp to drop to 140. Just my two cents.


--------------------
- Need help making a new substrate recipe from scratch? Try EdgarPoe's Substrate Recipe Calculator
- Designing or building a laminar flowhood? Try the Laminar Flowhood Calculator & Blower CFM Chart

"The world was made for the dead."
-Flannery O'Connor, "The Violent Bear It Away"

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Re: Oyster bucket prep, dirty tek fafo [Re: EdgarPoe]
    #28915951 -

EdgarPoe said:
Ah, ok. I must've misunderstood you on the lime.

Pasteurization at 140 seems doable. I've never done it at that temp, nor am I an expert at the process. I just do what works. I would recommend bringing the water to boil before adding the straw and allow the temp to drop to 140. Just my two cents.


A boil first sounds good to me. That's 212°F. It's going to drop to under a simmer quickly.

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Re: Oyster bucket prep, dirty tek fafo [Re: aShogunNamedMarcus] * 1
    #28916053 -

I think 180f is the correct starting point. 140-160 is proper past. Temps. If it stays too hot for too long you'll over pasteurize and leave the straw open to contams. You can add cold water to bring the temp down if the starting temp is above 180 after the straw goes in.

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Re: Oyster bucket prep, dirty tek fafo [Re: deadmandave]
    #28916479 -

deadmandave said:
I think 180f is the correct starting point. 140-160 is proper past. Temps. If it stays too hot for too long you'll over pasteurize and leave the straw open to contams. You can add cold water to bring the temp down if the starting temp is above 180 after the straw goes in.


I read your post right before I started and did just that. First batch is in the pot right now at 159F.

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Re: Oyster bucket prep, dirty tek fafo [Re: aShogunNamedMarcus]
    #28916740 -

I'm intrigued in how this turns out

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Re: Oyster bucket prep, dirty tek fafo [Re: aShogunNamedMarcus]
    #28916901 -

All stuffed into buckets now.

Even though one jar had water pooled in the bottom, there were no odd smells or colors or slime.

I took some grain from each jar to agar to observe and maybe get some transfer experience.

Seemingly reputable folks do their cook @ either 140-160, or 160-170 F for 1 to 2 hours.

I read that temps over 180F can caramelize sugars in the straw, which could invite contams.

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Re: Oyster bucket prep, dirty tek fafo [Re: aShogunNamedMarcus] * 1
    #28917097 -

I don't think caramelizing the sugars in straw would be much of a problem at those temps or time. but what does happen over 180 or even for an extended period at 160 is the thermophilic bacteria that would otherwise hold the straw stable for a couple weeks die, leaving the substrate open as a blank canvas for any mold that lands on it.

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Re: Oyster bucket prep, dirty tek fafo [Re: deadmandave] * 1
    #28917206 -

I always pasteurized barrels at 165F.

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Re: Oyster bucket prep, dirty tek fafo [Re: DERRAYLD]
    #28917450 -

165 is good. How long do you keep that temp? I think one hour is the right amount of time and two is too long.

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Re: Oyster bucket prep, dirty tek fafo [Re: deadmandave]
    #28917750 -

deadmandave said:
I don't think caramelizing the sugars in straw would be much of a problem at those temps or time. but what does happen over 180 or even for an extended period at 160 is the thermophilic bacteria that would otherwise hold the straw stable for a couple weeks die, leaving the substrate open as a blank canvas for any mold that lands on it.


Wow, that's info you don't get when just googling how to do things.

My aim was 160F for 1.5 hr

I kept both batches @ 156-163F

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Re: Oyster bucket prep, dirty tek fafo [Re: deadmandave] * 1
    #28918247 -

deadmandave said:
165 is good. How long do you keep that temp? I think one hour is the right amount of time and two is too long.


I used to do 1.5 hrs and then hoist to drain and cool.

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Re: Oyster bucket prep, dirty tek fafo [Re: aShogunNamedMarcus]
    #28922345 -

Update on the grain sample taken from each jar. Without having taken several samples of each, I can't draw much conclusion about when the contams were introduced. I took the samples mainly as a way to practice doing transfers to agar in the SAB.



Two on the left obviously contam. The right two look better but the top-right has a sketchy looking pattern.

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