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Re: Penis envy isolation part 3 [Re: Tormato]
    #25350154 -

no.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25350219 -

verum subsequentis said:
updated op with

BEHOLD








What kind of grain is being used here?

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Re: Penis envy isolation part 3 [Re: Skipio]
    #25350227 -

milo


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25350264 -

verum subsequentis said:
no.


Well...


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Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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Re: Penis envy isolation part 3 [Re: Tormato]
    #25350282 -

Nice thread :lowfive:


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Remember to be respectful to each other, be kind, and enjoy life in a responsible way :happyheart:


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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25350497 -

verum subsequentis said:
milo


Oh, "milo" is a grain. :smile:
Excellent.

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Re: Penis envy isolation part 3 [Re: Skipio]
    #25350681 -

Skipio said:
verum subsequentis said:
updated op with

BEHOLD








What kind of grain is being used here?


Just noticed, I like the wedge on the side, you just shift the grains by tipping jar? It looks like it would colonize faster that way.


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My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.

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Re: Penis envy isolation part 3 [Re: BigSurMoon]
    #25350774 -

i think the idea that it will colonize faster is incorrect. why would having it on the side cause it to colonize faster? every milometer of agar against the glass represents a couple less colonized grain to distribute upon the first shake.

my motivations were pure vanity. i just wanted that picture to look nice. i like to watch them grow too.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


Edited by verum subsequentis (07/27/18 07:02 PM)

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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25350782 -

oh, and yeah just get it over there. i don't know why this trips folks up. i've seen people discussing how to manage to get your agar on the side. what gives? just shake what the good lord gave ya but do it tactfully.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25351234 -

I thought it might be better cause only one side of the wedge has exposed myc(at least mine do), so if it was pushed up by grains it would then have more contact surface. Ive just been setting wedges on top and sometimes they dont sit flat. Some ive had not colonize, and or take forever to catch on. But i think how old the plate is made that difference also. Just thought I might have picked up on a trick, thanks for the clarification.


--------------------
My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.

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Re: Penis envy isolation part 3 [Re: BigSurMoon]
    #25351254 -

i never leave them on top and would not recommend it. get that thing in the grain.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25351275 -

verum subsequentis said:
i never leave them on top and would not recommend it. get that thing in the grain.


Good to know, I got some to do tomorrow, i will get em down in there. Thanks much!


--------------------
My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.

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Re: Penis envy isolation part 3 [Re: BigSurMoon]
    #25358945 -

Okay, first of all....I really was looking forward to more pictures, I am living vicariously through your penis gene journey.  Soon, I hope.

I need some more advice, and you have been especially responsive and helpful, much appreciated by the way! and it does pertain to your thread and methodology.

You inspired me to really seek isolates, and helped me to understand what that meant and what it would really take.  So, I ordered some petri dishes per your advice and agar mixing/pouring flasks (per Bod's advice/agar tek found here).

Today, I did the 3rd transfer of some Treasure Coast and Golden Teacher, I really loved how they looked pretransfer but the pictures were garbage in the SAB with the Pasty plates.  I am currently trying to take pictures with the plate upside down (SAB not flow hood) and using the forward facing camera on my phone, to prevent contams, this means quality is lower and all pics are currently inadvertently back lit due to my work environment. 

This, and the arrival of my flasks got me thinking, I use Pasty plates because of my limited resources and situation, specifically my Still Air Box.  With my flask and petri's when they arrive, can I safely pour contam free plates in a SAB?*edit*I did search and find that others are using a SAB and pouring plates, I would still like to know your opinion and any specific tips you may have used before you had a flow hood?

sorry, very talkative/typative this evening, apparently kratom makes me ramble, but very effective as a pain killer alternative

Edited by Ginge (07/31/18 09:20 PM)

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Re: Penis envy isolation part 3 [Re: Ginge]
    #25359104 -

yes, you can pour perfect agar petris in a sab. i don't like doing it but it absolutely can be done. just follow standard sterile sab tek. hmmm. maybe you don't know what that means. um... how bout this. let me know if you have any specific questions and i'll answer them. i'm just not really up to writing an entire sterile tek write now.


here is bodhi pouring in a sab


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25359149 -

verum subsequentis said:
i never leave them on top and would not recommend it. get that thing in the grain.


:thumbup: Correcting...

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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25361123 -

Thank you.  Hopefully the petris arrive soon.  I just did a 3rd transfer still using Pasty plates and all looks well so far.  In my plates I have had 3 contaminations, 2 of which were from the same host transfer.  Out of maybe 100 transfers.  So, I have a lot to learn, but I think my sterile technique is in a good starting place.  Once I have some plates to photo I will post some Golden Teacher and Treasure Coast cultures.


--------------------
Ginge



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Re: Penis envy isolation part 3 [Re: Ginge]
    #25361188 -

sexy plates and specially the jars.
Excited to see a cannopy of P.E. isolate.
Thats some hard perserverance with art :bow2:

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Re: Penis envy isolation part 3 [Re: pacmanbreed] * 1
    #25361196 -

How's the grain looking? Been watching this... silently lol (like a stalker (just fucked that up) )


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🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: Penis envy isolation part 3 [Re: BigSurMoon]
    #25361208 -

BigSurMoon said:
Ive just been setting wedges on top and sometimes they dont sit flat. Some ive had not colonize, and or take forever to catch on.



I make as many transfer plates as I need jars (+1 (just in case) ), but to 60mm plates,  cut them into 9 by scoring in a tic tac toe pattern, scooping them out directly over the mouth of the jar (making sure not to  move too quickly(not slow either,smooth),or have much directly over the open jars),and I shake the living hell out of them immediately after finishing up with all the SAB I'm doing at the time... i use an entire plate for each jar, and usually see signs of recovery within 24, but 36-48 wouldn't worry me... good way to test viability IMO... using softer agar, I've seen pint jars finish in 4 days


--------------------
🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: Penis envy isolation part 3 [Re: Caps McGee]
    #25361745 -

Caps McGee said:
BigSurMoon said:
Ive just been setting wedges on top and sometimes they dont sit flat. Some ive had not colonize, and or take forever to catch on.



I make as many transfer plates as I need jars (+1 (just in case) ), but to 60mm plates,  cut them into 9 by scoring in a tic tac toe pattern, scooping them out directly over the mouth of the jar (making sure not to  move too quickly(not slow either,smooth),or have much directly over the open jars),and I shake the living hell out of them immediately after finishing up with all the SAB I'm doing at the time... i use an entire plate for each jar, and usually see signs of recovery within 24, but 36-48 wouldn't worry me... good way to test viability IMO... using softer agar, I've seen pint jars finish in 4 days


Sounds good

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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