Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   MagicBag.co Certified Organic All-In-One Grow Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds High THC Strains   North Spore Bulk Substrate   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinealtan
Stranger
Registered: 11/02/03
Posts: 1
Last seen: 19 years, 11 months
amoxicillin clavulanate - or other antibiotics
    #2064968 - 11/02/03 12:55 PM (20 years, 4 months ago)

I was wondering if Augmentin or another antibiotic could be dissolved and then sprayed onto the mycelium or mushroom to prevent diseases - would it work, or just end up killing the shroom too?

Extras: Filter Print Post Top
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: amoxicillin clavulanate - or other antibiotics [Re: altan]
    #2065185 - 11/02/03 01:38 PM (20 years, 4 months ago)

It would depend on how much, but that'll only prevent bacterial contamination....

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

Extras: Filter Print Post Top
Offlinewalk
ignorantwhiteman

Registered: 06/22/03
Posts: 123
Loc: Dunedin, New Zealand
Last seen: 16 years, 10 months
Re: amoxicillin clavulanate - or other antibiotics [Re: micro]
    #2065640 - 11/02/03 04:40 PM (20 years, 4 months ago)

Even at lower temp than 37'c and with fairly specific substrate, is that a good idea? Breeding bacteria that resist amoxicillin?

Edited by walk (11/02/03 04:44 PM)

Extras: Filter Print Post Top
Offlinepluteus
level-9 deviant

Registered: 08/12/03
Posts: 170
Loc: London area, UK
Last seen: 17 years, 4 months
Re: amoxicillin clavulanate - or other antibiotics [Re: walk]
    #2068490 - 11/03/03 05:49 PM (20 years, 4 months ago)

Stamets reccomends 0.1 gram of gentamycin sulphate per litre of agar media to help fight bacterial contamination. I only use this recipie when I am cloning mushrooms from the wild. It is not a very nice substance to work with, and is quite expensive, but it usually decreases the bacterial contamination rate substantially (although my clones off of dung usually contam quickly). I don't worry about breeding resistant strains, cos if something contams, I kill it and throw it away, but if a culture survives, I get it onto non-antibiotic media asap.
I've tried all kinds of H2O2/antibiotic mixes for wild clonings, but have no clear-cut winning formula to report. Also from personal experience I think you'd be surprised how many little bugs from the depths of the rainforest are already resistant to the latest antibiotics !

Extras: Filter Print Post Top
Offlinewalk
ignorantwhiteman

Registered: 06/22/03
Posts: 123
Loc: Dunedin, New Zealand
Last seen: 16 years, 10 months
Re: amoxicillin clavulanate - or other antibiotics [Re: pluteus]
    #2068590 - 11/03/03 06:34 PM (20 years, 4 months ago)

Isnt amoxicillin clavulanate a antibiotic for human treatment?

Extras: Filter Print Post Top
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: amoxicillin clavulanate - or other antibiotics [Re: walk]
    #2068632 - 11/03/03 06:55 PM (20 years, 4 months ago)

Yeah -- and it's not good to be eating it (like if you're spraying it on your mushrooms) if you don't need it. An antibiotic is an antibiotic, though. The only real difference is the preperation -- a lot of antibiotics aren't soluble in water.

The reason gentamycin is good is because you can autoclave (or pressure cook it) -- I think it's the only antibiotic like that (or at least the only one I've used.)

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

Extras: Filter Print Post Top
Offlinezeronio
Stranger
Male

Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 7 years, 6 months
Re: amoxicillin clavulanate - or other antibiotics [Re: micro]
    #2079386 - 11/07/03 12:51 AM (20 years, 4 months ago)

Anno showed the there are some benefits of using Penicillin and Streptomycin in agar even if they're not supposed to be autoclavable.

Penicillin and streptomycin in agar

Extras: Filter Print Post Top
InvisibleZen Peddler
Male User Gallery

Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: amoxicillin clavulanate - or other antibiotics [Re: zeronio]
    #2083014 - 11/08/03 02:03 AM (20 years, 4 months ago)

however, ofcourse antibiotics have little affect on mold contaminants - they only lesson the chances of bacteria contaminants, which can usually be isolated out of the picture anyway.


--------------------

Extras: Filter Print Post Top
Offlinezeronio
Stranger
Male

Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 7 years, 6 months
Re: amoxicillin clavulanate - or other antibiotics [Re: Zen Peddler]
    #2083073 - 11/08/03 02:58 AM (20 years, 4 months ago)

Its easier to isolate away from bacteria when you're streaking spores then when you're trying to clone. Most of my failed cloning attempts failed because of bacteria that encircled the mushroom tissue. However peroxide is quite efficient in that case.

Extras: Filter Print Post Top
InvisibleG a n j a
Pictish and proud
 User Gallery

Registered: 12/03/02
Posts: 7,860
Loc: Zone ate
Re: amoxicillin clavulanate - or other antibiotics [Re: zeronio]
    #2224954 - 01/07/04 02:07 AM (20 years, 2 months ago)

I wonder if i can add amoxicillin to cooling agar mixes, as its a lot
cheaper and easyer for me o get hold of than gentamycin.
Does anyone know if its water soluble, or what temps break it down?
As i need an antibac for starting wild prints  :crazy: and already have
amoxicillin.


--------------------
er

Extras: Filter Print Post Top
InvisibleG a n j a
Pictish and proud
 User Gallery

Registered: 12/03/02
Posts: 7,860
Loc: Zone ate
Re: amoxicillin clavulanate - or other antibiotics [Re: G a n j a]
    #2225132 - 01/07/04 05:04 AM (20 years, 2 months ago)

Ah, found my awnser in the thread linked in this thread :smile:


--------------------
er

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   MagicBag.co Certified Organic All-In-One Grow Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds High THC Strains   North Spore Bulk Substrate   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Antibiotic agar. Bumbaclotjohnson 1,855 6 02/04/15 12:30 PM
by tscott1632
* Gentamycin Sulfate Antibiotic Powder? cArcace-x 1,392 11 08/07/14 07:20 AM
by Eskeer
* Agar Antibiotic Hesy-Ra 1,564 3 11/23/11 02:39 PM
by blackout
* % Gentamycin in Spore Syringe? Joshua 1,750 11 04/24/03 10:32 PM
by Odin 13
* antibiotic syringe? felixhigh 1,611 4 05/14/02 08:28 PM
by felixhigh
* Antibiotic concentrations in Agar cheesenoonions 3,956 9 12/29/02 11:40 PM
by Zen Peddler
* can gentamycin sulphate be injected into lc after its been pressure cooker? Biffzilla 1,048 8 07/13/12 07:38 AM
by Mateo
* Clindamycin as an Antibiotic for MEA
( 1 2 all )
Humbled 2,748 27 12/28/09 02:02 PM
by Humbled

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
1,905 topic views. 0 members, 4 guests and 5 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.018 seconds spending 0.004 seconds on 12 queries.