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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Re: Senescence in Isolation [Re: SwampTromper]
    #14091285 -

Another misunderstood aspect is chronological time, not just the amount of cell divisions.  A single kernel of colonized grain can be expanded to fill a large truck of grains without senescence if one works fast.  However,if you took each jar of grains and let it sit for a month before making the transfer, you wouldn't get nearly as far, even if they were refrigerated.  This is why we keep the masters on test tubes and don't begin the expansion until the time comes.
RR


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Re: Senescence in Isolation [Re: RogerRabbit]
    #14091808 -

so when you pull a test tube and make a new plate, will the test tube that was pulled  be shot?


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Re: Senescence in Isolation [Re: cyanara]
    #14093217 -

No.  I keep them for years and years.  I always pull cultures from my oldest source, which will deliver the youngest cell lines.  If you'll put wood in your culture slants, they'll last up to a decade if opened once a year in front of a flowhood to let in some fresh air.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Senescence in Isolation [Re: RogerRabbit]
    #14093734 -

RogerRabbit said:
if you took each jar of grains and let it sit for a month before making the transfer, you wouldn't get nearly as far, even if they were refrigerated.  This is why we keep the masters on test tubes and don't begin the expansion until the time comes.
RR


You are saying that you have to transfer the mycelium while its in its most vigorous stage of growth?

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Re: Senescence in Isolation [Re: mycocozm]
    #14094267 -

Yup, that is if you want to make half a dozen or more grain to grain transfers at a 1 to 20 ratio.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Senescence in Isolation [Re: RogerRabbit]
    #14119000 -

You go 1:20 g2g? Is your succeess rate still comparable to a lower ratio or is it compromised? I guess i'll just have to experiment and find out although i never go over 1:10.

Then again i havent been doing g2gs for twenty years. What would you reccomend? I have a pretty low contam rate.

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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