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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Using Cardboard in place of agar for wild prints.
    #5254584 -

Can you use moistened cardboard to germinate spores collected in an unsterile environment?  I'm just beginning to play around a bit with cardboard, and I'm interested in learning more about its magikal powers.  Thanks.  :fairy:


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Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!

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Re: Using Cardboard in place of agar for wild prints. [Re: GnuBobo]
    #5255579 -

You can.
I have soaked it in dark h/poo drain/off, then PC'ed it & done well.
(in a perti).


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Re: Using Cardboard in place of agar for wild prints. [Re: agar]
    #5256713 -

Whats PCing Agar old chum?


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"Odrade read the word silently and then aloud.
"Arafel."
She knew this word.Reverend Mothers of the tyrants time had impressed it into the Bene Gesserit consciousness,tracing it's roots to the most ancient sources.
"Arafel:the cloud darkness at the end of the universe.""

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Re: Using Cardboard in place of agar for wild prints. [Re: GnuBobo]
    #5259773 -

he means pressure cooking, which according to stamets is unneccessary..

i have some cardboard agar going now, cut a circle of cardboard out the diameter of the jar i was using, softened it up with some warm water, strained most of the water off and placed in it.. i dropped a few drops of spore solution and got some white fuzz starting but not much yet...

in stamets new book mycelium running he shows a picture of taking a spore print from a wild mushroom onto this moistened cardboard, and just letting it grow... should work fine

i have some woodlovers colonizing cardboard right now, tearing right threw it.. i inoculated that with agar wedges, i had an agar plate that grew out maybe 3/4 of the way, then contam'd along the edges.. in my kitchen, with no sterility at all, i cut out wedges using a unsterilized butter knife, and placed a wedge each onto 3"x3" squares of moistened cardboard.. all colonized completely, one showed some greenmold growth on the edges of where the agar was, but the growth stopped.. i have since put these 3x3 squares, now fully colonized, into pieces of cardboard maybe 10x20", this time i layered them inbetween, one layer of cardboard, then a colonized piece, then another layer of cardboard, then another colonized piece..

my first experiments have been going great... i first moistened the cardboard w/ cold water and that worked fine, i read in mycelium running after that he used warm water and that helped soften up the cardboard so you could peel away to get into the corrugated part(IT IS VERY IMPORTANT(i guess) TO USE CORRUGATED CARDBOARD)..

so far all the cardboard experiments have been going awesome and i look forward to continuing..


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"listening for the secret.. searching for the sound.."

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Re: Using Cardboard in place of agar for wild prints. [Re: mattymonkey]
    #5360311 -


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Re: Using Cardboard in place of agar for wild prints. [Re: Hotnuts]
    #5362346 -

having issues myself getting spores to germinate on cardboard, is this what you did hotnutz? how did you do it?


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"listening for the secret.. searching for the sound.."

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Re: Using Cardboard in place of agar for wild prints. [Re: Hotnuts]
    #5364155 -

hotnutz said:



bravo

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Re: Using Cardboard in place of agar for wild prints. [Re: mattymonkey]
    #5367817 -

It would be allot more sure fire if you germinate on grain then just inocultate that cardboard with kernels.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Re: Using Cardboard in place of agar for wild prints. [Re: Psychoslut]
    #5381122 -

Yep. Agar said it. Plain carboard sorta worried me with spore germination. So, I used potato water from some I boiled up to eat to add to the sterilization process. I simply cut carboard disks by tracing the bottom of petris and then added the disks to the potato water with a drop of Karo as well. I put this stuff in a microwavable container with a polyfill plug stuck into the lid so the container can breath during the micro run. Just let the simmer for a minute and that's plenty. Inoculate in a gloove box and go. You'll have to load the sterilized disks into the petris with sterile tweezers. It sounds like a pain, but it's simple.

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Re: Using Cardboard in place of agar for wild prints. [Re: Hotnuts]
    #5381127 -

The pic above is actually a clone. Sorry, I jumped the gun there. It indeed works for germination of spores too.

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Re: Using Cardboard in place of agar for wild prints. [Re: GnuBobo]
    #14108947 -

I found out that it's a lot easier when you're not infected with Trichophyton and live in a house that is infected with some kind of black mould :grin:.


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"No matter how subtle the wizard, a knife between the shoulder blades will seriously cramp his style."
— Steven Brust

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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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