Home | Community | Message Board


World Seed Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar, Petri Dish

Jump to first unread post. Pages: 1
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 3 years, 8 months
Kuehneromyces mutabilis on cardboard agar
    #3065782 - 08/29/04 06:10 PM (13 years, 3 months ago)

Kuehneromyces mutabilis is a close relative of Pholiota nameko which grows on dead broadleaf trees and is a very good edible.



Since I got a spore print of Kuehneromyces mutabilis from speeker ( thanks again :wink: )  I decided to put the mycelium on cardboard agar.

The recipe for the cardboard agar was inspired by the ATWAR's cardboard cloning tek on shroomery and this thread on mycotopa.

Here is what I did:

I peeled of the first layer of the corrugated cardboard and soaked it in the following solution:

- 500 ml h20
- 20  g dextrose
- 1  g yeast
- tiny bit of soy sauce

After soaking it was put into a glass petri dish and covered a bit with that solution and then closed again.
Then it was heated in the microwave oven till the cardboard was moist but not wet anymore.
After that a bit of mycelium of K. mutabilis was transfered onto the cardboard agar in the glovebox.
I think it is important that the agar has a good contact with the surface of the cardboard, so that it can take hold easily.

Here is a pic from the top side:



And here is one from the back side:




I also got a tiny piece of wood where the mycelium of K. mutabilis was growing on and put it on some wet cardboard.
But this time I did have not added any nutrients to the cardboard.
The mycelium took over to the cardboard but it is growing much slower and is only recognizable by the change in color.
You can see that K. mutabilis is a white rot fungus :smile::





And here you can see how the mycelium of K.mutabilis looks on normal agar:



Update: Now the mycelium has grown out the cardboard and looks like that:





Edited by ragadinks (10/08/04 07:52 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCryogeniczV
what?
Male User Gallery

Registered: 07/01/04
Posts: 2,421
Loc: Oregon
Last seen: 1 day, 17 hours
Re: Kuehneromyces mutabilis on cardboard agar [Re: ragadinks]
    #3190051 - 09/28/04 03:21 AM (13 years, 2 months ago)

You fucking Rock Raga!


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 3 years, 8 months
Re: Kuehneromyces mutabilis on cardboard agar [Re: Cryogenicz]
    #3190130 - 09/28/04 03:38 AM (13 years, 2 months ago)

Thanks :wink:
The culture has already grown out the cardboard and I have put it in the fridge ...


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

Similar ThreadsPosterViewsRepliesLast post
* Kuehneromyces mutabilis pinning in jar ragadinks 1,283 4 01/20/05 04:22 PM
by firstmatefluff
* FreeSporePrints - Book your SP [Pholiota,Lentinus,Agaricus,Ophyllum,Stropharia,Kuehneromyces, etc..] FreeSporePrints 1,614 6 05/15/06 11:11 PM
by shroomydan
* Looking for Kuehneromyces (Pholiota) mutabilis culture or spores denger 1,121 5 09/23/08 01:42 PM
by FreeSporePrints
* Using Cardboard in place of agar for wild prints. GnuBobo 2,276 10 03/12/11 03:07 PM
by Fnorgl
* K. Mutabilis
( 1 2 3 all )
falcon 5,747 46 09/12/12 03:57 AM
by Javadog
* Reishi spores ---> BRF agar substitute zeegos 2,281 18 10/30/06 11:25 PM
by CptnGarden
* cloning a storebought oyster w/o agar? kingjames488 2,147 15 04/29/09 03:11 PM
by jjb007
* Blue oysters Laundry Basket tek Cardboard only substrate update with pics
( 1 2 all )
coolbeverage 7,418 21 07/14/13 02:16 AM
by MattyBear

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte
2,554 topic views. 4 members, 8 guests and 4 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
High Mountain Compost
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.006 seconds on 19 queries.