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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Problems with strains
    #10947472 -

K, so I got mex-a, temp, PE, and Alt#7.  Mex-a is a super quick colonizers, followed by Alt#7, PE is going slow as fuck(it is 15% colonized when the mex-a is over colonized; about two weeks and the jar is only half pint of WBS), and the temp never really germinated, just got some grayish mycelium with blackish/green specks in the center of the growth.

Questions: I know PE is the new revered strain, is it suppose to grow this slow?  The syringes were not nearly as thick as the mex, but thats suppose to be normal

anyone had problems with Temp? or think i just got a bad syringe?


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Can't deal with the stubborn and the ignorant.  Good bye to those who were cool.

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Re: Problems with strains [Re: guitarpah]
    #10947497 -

I've always found PE to be slightly slower to colonize and less resistant to contam tho not as slow as yours seems to be going. Unfortunately colonization times and other properties change from batch to batch. Have patience. As for the last strain you mentioned, throw that away and don't open it inside, go to the woods if need be... that grey fuzz is either mold or dead myc and the green/blue stuff most certainly is mold.

Cheers!


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___________________________________________________________

Whether you're here to learn or teach the art of mycology,
do so with an open mind.

My posts are 100% fictional... 
I'm just makin it all up! :wink:

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Re: Problems with strains [Re: Bejeezis]
    #10947662 -

The secret to strains is to isolate one on many agar plates... each sub-strain will keep sectering in a multispore inoculation. So unless you have isolated one you don't know what what to expect.

some of RR's notes on strains

edit:

subject of mixing/crossing strains... off subject


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Edited by UFOz8MyGoat (08/27/09 11:34 AM)

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Re: Problems with strains [Re: UFOz8MyGoat]
    #10947701 -

You don't isolate looking for one super rhizomorphic strain. You isolate down to single sectors and then fruit out each one to determine the best performer. When you transfer mycelium to a grain master, the original petri dish the mycelium was taken from is placed into a clean refrigerator. By doing this, when you find the best performing strain, you then go back to your well marked petri dishes, thus your original P1 culture. This petri dish can be used to inoculate a few test tube slants that can be incubated for a week, then placed in cold storage. Whenever you need mycelium, a tiny piece the size of a grain of rice can be taken from the test tube and put on agar to grow out, while the test tube is placed back into the refrigerator. These stored test tube cultures preserve the low P value of your isolated strain for years.
I have a complete video tek on strain isolation and master slant preparation and use already filmed. I'll release it when I get the rest of the teks filmed, and editing completed. Hopefully soon. STRAIN ISOLATION VS CLONING

*Right. You isolate every single strain you can, and then fruit them all. While they're fruiting, the 'master' from each strain is in the refrigerator. After you determine the best performers, you go back to the appropriate masters and get them out. Those are the ones you transfer to culture slants for long term storage. Rhizomorphic mycelium tends to fruit better than cottony mycelium, but a single swipe of spores on agar is likely to generate fifty or more individual sectors(strains), and half or so of those will be rhizomorphic. Those are the ones I keep for fruiting, and discard the cottony sectors. ISOLATING STRAINS

*Don't wait for the first dish to fully colonize. As soon as it begins to grow, transfer mycelium away from the point of cloning to new dishes. This way, you can isolate healthy mycelium away from contaminants. You won't be doing strain isolation on a clone, so don't bother looking for rhizomorphs. Just get healthy mycelium. ISOLATING

*For strain isolations, you can't beat the three section dishes. You're only letting the mycelium grow for a few days before making the next transfer, so you can to three times the amount of work on each dish. The four section dishes are great too. I don't even order the plain dishes anymore. STRAIN ISOLATION

*Cloning a fruit from within a cluster will provide a strain that will produce clusters. It matters not which fruit you choose from within the cluster, but you'll have better success if you'll clone while they're still small and rapidly growing. CLONING/ISOLATING

*It's extremely rare for rhizomorphic isolates to be none fruiting. STRAIN ISOLATES


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Re: Problems with strains [Re: UFOz8MyGoat]
    #10947739 -

you get a medal for the longest post in history.
quite informative.


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ephemeral anomalous

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Re: Problems with strains [Re: prismism]
    #10947752 -

lmao sorry been doing some learning...it seems there's so much i've had wrong and not understood ... RR is a walking encyclopedia of mycology wow lol


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Re: Problems with strains [Re: UFOz8MyGoat]
    #10948241 -

Pe from what I've noticed is a slower colonizer, but a fast fruiter. So it makes up on that end. Thats at least what I've noticed from my experience with PE.


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"Lennox Lewis, I'm coming for you man. My style is impetuous. My defense is impregnable, and I'm just ferocious. I want your heart. I want to eat his children. Praise be to Allah!" An American Hero Iron Mike!

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Re: Problems with strains [Re: UFOz8MyGoat]
    #10950523 -

UFOz8MyGoat said:
lmao sorry been doing some learning...it seems there's so much i've had wrong and not understood ... RR is a walking encyclopedia of mycology wow lol



how did you chang your name????????


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Re: Problems with strains [Re: feelfunny]
    #10950870 -

I purchased a name change for 10 dollars :smile:


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Re: Problems with strains [Re: UFOz8MyGoat]
    #10950910 -

why did you change it? and you became a supporter


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Re: Problems with strains [Re: feelfunny]
    #10951004 -

I didn't like my state being displayed lol plus all the BS from otd ... yea i like the name colors and perks


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Re: Problems with strains [Re: UFOz8MyGoat]
    #10951037 -

good point and like the color to. the fam is having a spore give away to anyone who becomes a 1 or more month supporter, i know you wanted out but i will send 1 to ya for getting it anyway if you want. PM me the address you want it to if you want


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Re: Problems with strains [Re: feelfunny]
    #10951152 -

hmm. my PE colonized fast. i have a 12quart dishtub 3rd day fruiting. i hope it fruits fast too

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Re: Problems with strains [Re: wattballasts]
    #10951220 -




colonized jars in 12 days spawnd to coir and colonized that in 5 and then put thin caseing layer and 10 days fruits


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Re: Problems with strains [Re: feelfunny]
    #10951471 -

nice my friend is trying to start some pf red spore and PE(u) on agar

Edited by UFOz8MyGoat (08/27/09 09:44 PM)

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