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 Mycodood
Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 23 days, 1 hour
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 Cloning and Karo LC 
#7213633 - 07/23/07 01:40 PM (13 years, 7 months ago) |
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Has anyone successfully cloned a piece of mushroome
tissue to Karo LC successfully?
I am working on one now and and I added my specimen
6 days ago and it looks good but just really slow.
Is this normal for tissue cloning?
When I do multipore LC's they take off after about 6 days.
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 GoodbyeOrb
Self-SacrificingPotency Tester
Registered: 04/09/07
Posts: 5,176
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Re: Cloning and Karo LC [Re: Mycodood]
#7213906 - 07/23/07 03:22 PM (13 years, 7 months ago) |
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:scratcheshead:
I'm no pro, but I think you are supposed to clone to agar when using a fruit wedge.
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TheFish
Shakedown Street
Registered: 10/07/03
Posts: 440
Last seen: 5 years, 7 months
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Re: Cloning and Karo LC [Re: GoodbyeOrb]
#7213925 - 07/23/07 03:28 PM (13 years, 7 months ago) |
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--------------------
"Comes a time when the blind man takes your hand,
Says, 'Don't you see?'
Gotta make it somehow on the dreams you still believe.
Don't give it up.
You got an empty cup only love can fill."
Jerry Garcia
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Mycodood
Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 23 days, 1 hour
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Re: Cloning and Karo LC [Re: GoodbyeOrb]
#7213947 - 07/23/07 03:37 PM (13 years, 7 months ago) |
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I thought it is possible to clone using LC too?
And agar is to isolate?
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Mycodood
Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 23 days, 1 hour
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Re: Cloning and Karo LC [Re: TheFish]
#7213949 - 07/23/07 03:39 PM (13 years, 7 months ago) |
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Quote:
TheFish said:
Cloning Guide For Newbs
This would be great if I hadn't posted that I ALREADY transfered to
LC.
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GoodbyeOrb
Self-SacrificingPotency Tester
Registered: 04/09/07
Posts: 5,176
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Re: Cloning and Karo LC [Re: Mycodood]
#7213953 - 07/23/07 03:39 PM (13 years, 7 months ago) |
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Well in that case....
Quote:
Mycodood said:
Has anyone successfully cloned a piece of mushroome
tissue to Karo LC successfully?
Obviously someone has had success,just read about dude that did it and wrote that tek. He also straight up quotes 7 - 10 days as inoculation time. Good luck.
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Mycodood
Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 23 days, 1 hour
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Re: Cloning and Karo LC [Re: GoodbyeOrb]
#7213994 - 07/23/07 03:52 PM (13 years, 7 months ago) |
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Yeah he uses Honey water.
My recipe is Karo. I wanted to know if anyone
using Karo. Wanted to know if it was a slow colonizer.
Slower than say honey or PD LC. I use PD lc all the time
but I tried Karo this time because I like the simplicity of it.
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TheFish
Shakedown Street
Registered: 10/07/03
Posts: 440
Last seen: 5 years, 7 months
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Re: Cloning and Karo LC [Re: Mycodood]
#7213999 - 07/23/07 03:54 PM (13 years, 7 months ago) |
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Have patience grasshopper. You seem like you're trying to nail down the incubation time to within a day.
Various factors create various results.
--------------------
"Comes a time when the blind man takes your hand,
Says, 'Don't you see?'
Gotta make it somehow on the dreams you still believe.
Don't give it up.
You got an empty cup only love can fill."
Jerry Garcia
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Mycodood
Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 23 days, 1 hour
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Re: Cloning and Karo LC [Re: TheFish]
#7214062 - 07/23/07 04:14 PM (13 years, 7 months ago) |
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I do have patiaence my friend. This hobby has taught me that.
My question is only if anyone has seen this type of
slow incubation with Karo specific LC's. If this turns out
to be a slower colonizer than some other medium I don't want to
waste my time with it. Because god knows time is a commodity with
this hobby.
I'm not trying to nail down colonization within a day.
I am curious as to what others get for results with the
variables I mentioned Tissue cloning > Karo LC.
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