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InvisibleCLuB99
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Registered: 10/26/99
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Troubles germinating Ps. mexicana/Ps. tampanensis
    #600338 - 04/06/02 04:13 AM (22 years, 2 months ago)

I tried on MYA agar and on a custom recipe (wich worked great with cubies and pantrops) made with honey, the broth of boiling amaranth and quinoa and some ground rye added.

More than 10 days since inoculation and still nothing.
Temps are fluctuating from 26 to 28 degree (c?)
i'm thinking to try the following recipes next , since i got both peptone and dextrose (tnx sporeworks!): MYPA, PDA plus peptone, OMYA, ...with peptone added.

any suggestions??

Edited by CLuB99 (04/06/02 04:20 AM)

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InvisibleAssHumper10K
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Registered: 11/11/01
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Re: Troubles germinating Ps. mexicana/Ps. tampanensis [Re: CLuB99]
    #600385 - 04/06/02 07:04 AM (22 years, 2 months ago)

In reply to:

any suggestions




Wait a few more days.


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Invisiblefeito
hillbillyhippykiller

Registered: 03/06/02
Posts: 75
Re: Troubles germinating Ps. mexicana/Ps. tampanensis [Re: CLuB99]
    #600450 - 04/06/02 09:35 AM (22 years, 2 months ago)

Yep. Wait.
My mexicanas took 15 days on agar, but a syringe was used to inoc it. MEA and PDA.
The Tamps started at day 10, and are VERY aggressive. From a print. Transfers take off immediately, and a plate can fully run in a week or 10 days. You should see the spawn run through compost!
I have pics of the Tampa around....old and new if you need them.

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InvisibleCLuB99
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Registered: 10/26/99
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Re: Troubles germinating Ps. mexicana/Ps. tampanensis [Re: feito]
    #600544 - 04/06/02 12:41 PM (22 years, 2 months ago)

i'd love to see your pics thanks...

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Invisiblefeito
hillbillyhippykiller

Registered: 03/06/02
Posts: 75
Re: Troubles germinating Ps. mexicana/Ps. tampanensis [Re: CLuB99]
    #600626 - 04/06/02 02:58 PM (22 years, 2 months ago)

This is Tampa after 1 transfer. Notice the knots....
and its crawling out of the dish!

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