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zeronio
Stranger

Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 7 years, 2 months

Microscope

#573144 - 03/08/02 01:00 AM (21 years, 8 months ago)

What kind of microscope and magnification would be suitable to study fungi characteristics like spore shape, hyphae, basidia, etc.
Various "myco" supply web sites sell everything from toy-like microscopes for 38$ up to professional ones for more than 1000$.
Does anybody have any experience with this?

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aaron
member

Registered: 10/17/99
Posts: 89

Re: Microscope

[Re: zeronio]
#573323 - 03/06/02 05:45 PM (21 years, 8 months ago)

You will need a good light microscope with magnifying capacity of at lest 400X and pfeferably 1000X with an oil-immersion lens. the eyepiece should be equipped with a calibrated micron bar.

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Suntzu
Geek

Registered: 10/14/99
Posts: 1,396
Last seen: 1 month, 9 days

Re: Microscope

[Re: zeronio]
#573328 - 03/06/02 05:51 PM (21 years, 8 months ago)

For most everything fungal I've looked at, oil immersion is not necessary. That means a scope with a 40X lense [400X magnification with the 10X eyepiece] is sufficient to identify mold conidia. Visualizing clamp connections is something I've never done, so that might be an oil-immersion application.

**also, checking out spore shapes/characteristics is most definitely an oil-immersion application**

I only mention the oil immersion as such scopes are significantly more expensive than those with just a 40X upper range [oil immersion is necessary with most scopes at 100X, 1000X with optical].

If you are serious about this, you will want to purchase dyes. . .lactophenol/cotton blue is good for fungi; put a drop on a slide, take a piece of CLEAR tape, lightly touch the culture with the sticky side [keep biosafety in mind here], lightly place the tape sticky-down onto the dye, put another drop on the top of the tape, and place a coverslip overtop.

Visualize at 400X and you'll get something like this:

Penicillium sp.

Edited by Suntzu (03/06/02 05:54 PM)

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Mushrooms, Mycology and Psychedelics > Advanced Mycology

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