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Offlinezeronio
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Microscope
    #573144 - 03/08/02 03:00 AM (19 years, 5 days ago)

What kind of microscope and magnification would be suitable to study fungi characteristics like spore shape, hyphae, basidia, etc.
Various "myco" supply web sites sell everything from toy-like microscopes for 38$ up to professional ones for more than 1000$.
Does anybody have any experience with this?


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Invisibleaaron
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Registered: 10/18/99
Posts: 89
Re: Microscope [Re: zeronio]
    #573323 - 03/06/02 07:45 PM (19 years, 7 days ago)

You will need a good light microscope with magnifying capacity of at lest 400X and pfeferably 1000X with an oil-immersion lens. the eyepiece should be equipped with a calibrated micron bar.


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OfflineSuntzu
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Re: Microscope [Re: zeronio]
    #573328 - 03/06/02 07:51 PM (19 years, 7 days ago)

For most everything fungal I've looked at, oil immersion is not necessary. That means a scope with a 40X lense [400X magnification with the 10X eyepiece] is sufficient to identify mold conidia. Visualizing clamp connections is something I've never done, so that might be an oil-immersion application.

**also, checking out spore shapes/characteristics is most definitely an oil-immersion application**

I only mention the oil immersion as such scopes are significantly more expensive than those with just a 40X upper range [oil immersion is necessary with most scopes at 100X, 1000X with optical].

If you are serious about this, you will want to purchase dyes. . .lactophenol/cotton blue is good for fungi; put a drop on a slide, take a piece of CLEAR tape, lightly touch the culture with the sticky side [keep biosafety in mind here], lightly place the tape sticky-down onto the dye, put another drop on the top of the tape, and place a coverslip overtop.

Visualize at 400X and you'll get something like this:



Penicillium sp.


Edited by Suntzu (03/06/02 07:54 PM)


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