Home | Community | Message Board

Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Microscope

Jump to first unread post. Pages: 1

Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 1 year, 5 months
    #573144 - 03/08/02 03:00 AM (15 years, 11 months ago)

What kind of microscope and magnification would be suitable to study fungi characteristics like spore shape, hyphae, basidia, etc.
Various "myco" supply web sites sell everything from toy-like microscopes for 38$ up to professional ones for more than 1000$.
Does anybody have any experience with this?

Post Extras: Print Post  Remind Me! Notify Moderator

Registered: 10/18/99
Posts: 89
Re: Microscope [Re: zeronio]
    #573323 - 03/06/02 07:45 PM (15 years, 11 months ago)

You will need a good light microscope with magnifying capacity of at lest 400X and pfeferably 1000X with an oil-immersion lens. the eyepiece should be equipped with a calibrated micron bar.

Post Extras: Print Post  Remind Me! Notify Moderator
 User Gallery

Registered: 10/15/99
Posts: 1,396
Last seen: 2 days, 2 hours
Re: Microscope [Re: zeronio]
    #573328 - 03/06/02 07:51 PM (15 years, 11 months ago)

For most everything fungal I've looked at, oil immersion is not necessary. That means a scope with a 40X lense [400X magnification with the 10X eyepiece] is sufficient to identify mold conidia. Visualizing clamp connections is something I've never done, so that might be an oil-immersion application.

**also, checking out spore shapes/characteristics is most definitely an oil-immersion application**

I only mention the oil immersion as such scopes are significantly more expensive than those with just a 40X upper range [oil immersion is necessary with most scopes at 100X, 1000X with optical].

If you are serious about this, you will want to purchase dyes. . .lactophenol/cotton blue is good for fungi; put a drop on a slide, take a piece of CLEAR tape, lightly touch the culture with the sticky side [keep biosafety in mind here], lightly place the tape sticky-down onto the dye, put another drop on the top of the tape, and place a coverslip overtop.

Visualize at 400X and you'll get something like this:

Penicillium sp.

Edited by Suntzu (03/06/02 07:54 PM)

Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Microscope

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Microscope. Seril 939 10 01/20/03 11:55 AM
by Anonymous
* Quality Microscope hills 1,074 3 12/17/05 04:02 PM
by hills
* Help Choose A MicroScope And Camera xburn 1,213 7 06/03/05 06:39 PM
by xburn
* microscope
( 1 2 all )
CryogeniczV 3,700 24 03/20/07 08:20 PM
by Meat_hod
* Microscope question MustardMan 2,238 9 01/07/07 08:28 PM
by fastfred
* Microscope purchase... kiss 1,345 12 01/14/06 07:29 PM
by kiss
* Microscope Modification ShroomNoob03 1,892 7 10/10/06 03:14 AM
by fastfred
* Microscope recommendations anyone? armedia 1,777 6 04/24/02 07:20 AM
by zeronio

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
882 topic views. 1 members, 2 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.007 seconds on 20 queries.