Home | Community | Message Board


Kraken Kratom
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Shroomery Sponsors Forum

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar, Petri Dish

Jump to first unread post. Pages: 1
OfflinepsilocyberV
old hand

Registered: 06/09/99
Posts: 1,809
Last seen: 28 days, 20 hours
P. mexicana Strain-A (Sclerotia) syringes are here
    #487430 - 12/11/01 03:27 PM (14 years, 11 months ago)

Post deleted by administrator.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMicronMagick
old hand

Registered: 10/17/01
Posts: 1,026
Post deleted by users_request [Re: psilocyber]
    #487446 - 12/11/01 03:41 PM (14 years, 11 months ago)

Post deleted by administrator.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinepsilocyberV
old hand

Registered: 06/09/99
Posts: 1,809
Last seen: 28 days, 20 hours
Re: P. mexicana Strain-A (Sclerotia) syringes are here [Re: MicronMagick]
    #487511 - 12/11/01 04:41 PM (14 years, 11 months ago)

Post deleted by administrator.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblealtarego
member
Registered: 10/25/01
Posts: 130
Re: P. mexicana Strain-A (Sclerotia) syringes are [Re: psilocyber]
    #487729 - 12/11/01 07:35 PM (14 years, 11 months ago)

PC -

Would you mind explaining how you came to isolate each of these two strains?

Is strain A a substrain of B (from a multispore innoculation of B?) Or are they both substrains of another?

Did you do a pure strain isolation from a sclerotia, call it the A strain, then fruit it?

Have you found that spores from both reliably mimic their parent strain?

I'm still trying (even after years of working with fungi) to sort out the subtleties of mushroom genetics, and any answer would help in that effort.

Best,
AE


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleOlgualion
Shaman-In-Training
 User Gallery

Registered: 11/14/01
Posts: 1,253
Loc: Currently Earth...
Re: P. mexicana Strain-A (Sclerotia) syringes are [Re: altarego]
    #487943 - 12/11/01 10:45 PM (14 years, 11 months ago)

I also would love to hear about this...

We have been hearing "workman is working on blablabla", I'd love to hear how he goes about doing it. Answering alterego's questions would give us some idea...

Thanks
Oq


--------------------
Study the past...
See the future...


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineWorkmanV
Psilocybe Microscopist
Male User Gallery

Registered: 03/01/01
Posts: 3,492
Loc: Washington, USA
Last seen: 1 year, 10 months
Re: P. mexicana Strain-A (Sclerotia) syringes are [Re: altarego]
    #488081 - 12/12/01 12:45 AM (14 years, 11 months ago)

Here is a picture of the original collection of 7 dried wild harvested mexicana caps as I received them. Usually I deal with sporeprints but the mushrooms didn't drop spores so I desperately requested and received the dried material for an attempt at spore extraction.



I started my experiments with the 2 caps labeled A and B in the photo. The were the best looking mature large caps. I especially liked the pronounced nipple on the A cap. I was successful with both attempts and retrieved clean mycelium after a few transfers in petri dishes.

Fruiting trials proved that the B-strain was a prolific fruiter and "A" only developed mushrooms belately and sparsely. Since I wanted spores I concentrated on the B-strain and all but ignored the A-strain. Fortunately, I let a friend work with both strains and he tipped me off on the sclerotia potential of "A".

So to answer the questions in order.

A is not a substrain of B. They were both collected individually from the wild at the same general locality and it is unknown how closely related they are.

A was fruited first, then tested for sclerotia ability.

Surprisingly enough, the mexicana strains seem remarkably stable from multispore innoculations. I have done more with the B strain and all isolates seem very similar with differences as easily attributed to environmental factors as to genetics. One thing of note is that noone (including myself) has isolated a decent invitro sclerotia former from the B-strain. It just doesn't seem to have the right genes regardless of the recombination expected with multispore culturing and isolation.

Less work has been done with the A-strain but at least sclerotia formation seems stable. This doesn't mean you shouldn't isolate the best strains possible from spores if you have the means to do so. It does mean that you shouldn't suffer too much from multispore inoculations if that is all you are set up to do.

There are 5 more mushrooms caps from that collection that need to be tested for desireable characteristics. Is it possible that I just arbitrarily chose the best fruiter and sclerotia former from the bunch, or is there something better hidden in those dry mushrooms?


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRyche HawkV
A Messenger
Male User Gallery

Registered: 03/01/01
Posts: 2,065
Re: P. mexicana Strain-A (Sclerotia) syringes are [Re: Workman]
    #488511 - 12/12/01 12:19 PM (14 years, 11 months ago)

Kudos to you guys.. a very creative idea.


--------------------
-Peace-
For all your legal mushroom spores and cultures visit  www.muShrooms.com


muShrooms.com is the new web site of
www.thehawkseye.com
Sacred Mushroom Spores since 1998


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleOlgualion
Shaman-In-Training
 User Gallery

Registered: 11/14/01
Posts: 1,253
Loc: Currently Earth...
Re: P. mexicana Strain-A (Sclerotia) syringes are [Re: Workman]
    #488514 - 12/12/01 12:21 PM (14 years, 11 months ago)

Awesome Workman,

So you actually extracted spores from the dried specimen to begin a multispore agar innoculation. Damn.. you are good!!

I'm definitely going to get a print of the A variety for dude to work with when I save up some money...

Oq


--------------------
Study the past...
See the future...


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Shroomery Sponsors Forum

Similar ThreadsPosterViewsRepliesLast post
* Sporeworks: May Sale, Psilocybe mexicana Strain-A!! psilocyberV 4,689 7 05/24/04 04:48 PM
by psilocyber
* Sporeworks: 50% off Psilocybe mexicana : Strain-B(TM) spore syringes! psilocyberV 1,432 5 05/12/06 02:35 PM
by Workman
* Sporeworks: FREE Ps. mexicana strain-A syringe offer! psilocyberV 2,246 17 09/28/04 02:21 PM
by psilocyber
* Sporeworks: Ps. mexicana strain-B and Jalisco prints in stock!! psilocyberV 1,509 3 04/05/05 09:13 PM
by ralphster44
* Sporeworks: Weekly Special, Ps. mexicana Strain-A only $19.95!! psilocyberV 908 2 07/06/05 12:46 AM
by TCatz
* Sporeworks: New Weekly Specials, Ps. mexicana Strain-A 50% off syringes! psilocyberV 746 1 05/02/05 06:46 PM
by tahoe
* New Ps. mexicana strain, new Agar Culturing Kits psilocyberV 641 2 12/14/02 10:24 AM
by psilocybinjunkie
* RalphstersSpores' A'-strain..... what do i have?
nolongerinuse
824 3 05/30/08 11:26 PM
by nolongerinuse

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: geokills, motaman, Prisoner#1
1,942 topic views. 0 members, 0 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Phytoextractum
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.045 seconds spending 0.004 seconds on 14 queries.