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 Workman
1999 Spore War Veteran
 
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 7 hours, 28 minutes
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 Unknown bluing possible Conocybe, Maryland, USA (updated with cultivation images) 
#4656425 - 09/13/05 06:12 PM (18 years, 7 months ago) |
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Ok, I only had about thirty minutes to do this, so the work is a bit sloppy. This is a mushroom collected by mikonn in Maryland in June 2005.
Original collection post
This is a preliminary look at some microscopic features of sample #10 labeled as "Liberty capish" Pointer is 8.4 microns wide.
  
I decided to start with this particular sample because it was large and the bluing on the stem was particularly visible. The gills are brown with a whitish fringe, the spores are large at 16+ microns in length and brown. The sterigmata are very long and darkly colored like the spores. Due to the poor quality of the sample preparation, the number of spores per basidium is unknown but assumed to be 4.
This sample was not properly rehydrated nor sliced in a manner to enhance the visibility of the microscopic features. A dry gill was merely ripped from mushroom and place on a slide with a drop of water and a cover slip. A better photo study will be done in the near future. Spores of this sample were also placed on agar for a cultivation attempt.
This preliminary observation rules out the genus Psilocybe but does not rule out the possibility of an active Conocybe. The large spore size does rule out the known active Conocybes, C. cyanopus and C. smithii which both have spores under 9 micrometers in length. I have no data on C. siligineoides but that species purportedly grows on wood. Conocybe tenera has large spores up to 14 microns in length but the stem is supposed to be fragile and these seem fairly tough (dry) and no mention is made of bluing at the stem base.
More features will need to be observed to confirm or rule out the genus Conocybe such as the cap cuticle. I am not as familiar with this genus and it may take me awhile to get a handle on this specimen. But the bluing is interesting.
 
Images added 10/13/05
Cap cuticle with round cells. Mycelium on agar isolated from gills of specimen #10
The cap cuticle is confirmed as cellular which rules out the genera Psilocybe and Galerina
Edited by Workman (01/02/06 04:41 PM)
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Re: Unknown bluing mushroom microscopy study [Re: Workman]
#4657790 - 09/14/05 12:00 AM (18 years, 7 months ago) |
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Might be a good specimen to try getting the dry tissue to grow. I've pulled out two year old dried mushrooms and coaxed them into coming back. Bear in mind, other non-active species blue as well. Good luck.
RR
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"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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Hotnuts
old hand
Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 6 days, 19 hours
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Re: Unknown bluing mushroom microscopy study [Re: RogerRabbit]
#4658656 - 09/14/05 07:27 AM (18 years, 7 months ago) |
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I didn't see the original post link there Workman. You can ignore the PM if you want....
Edited by hotnutz (09/14/05 09:47 AM)
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 EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
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Re: Unknown bluing mushroom microscopy study [Re: Workman]
#4661365 - 09/14/05 05:24 PM (18 years, 7 months ago) |
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I know he had a mixed collection, some like subbs, some like copelandias, some like liberty caps. Did he send you the Possible Copelandia also?
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 7 hours, 28 minutes
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Re: Unknown bluing mushroom microscopy study [Re: EonTan]
#4661901 - 09/14/05 07:36 PM (18 years, 7 months ago) |
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I was sent 79 specimens individually bagged. I haven't had the time to look at more than the first 10. I suspect there are several of everything.
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Research funded by the patrons of
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Reinvesting 25% of Sales Towards Basic Research and Species Identification  
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mikonn
me
Registered: 05/29/05
Posts: 192
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Re: Unknown bluing mushroom microscopy study [Re: Workman]
#4662106 - 09/14/05 08:12 PM (18 years, 7 months ago) |
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Sorry about sending so many. Didn't mean to over do it, just over enthusiastic I quess.
Looks very interesting though.
EonTan --I'm sorry I forgot about your interest in those others. If you are still interested I can rectify that.
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 7 hours, 28 minutes
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Re: Unknown bluing mushroom microscopy study [Re: mikonn]
#4662206 - 09/14/05 08:31 PM (18 years, 7 months ago) |
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No need to apologize. It is like Christmas to me. I am just a little swamped at the moment. I'll catch up and do a proper job on the whole lot soon enough.
I am pretty excited about this first one so far.
--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
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Re: Unknown bluing mushroom microscopy study [Re: mikonn]
#4664481 - 09/15/05 10:58 AM (18 years, 7 months ago) |
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No problems. As long as workman got some of each, I am sure he will get to them. 
Yes that first one is very interesting!!!
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Zen Peddler
Registered: 06/18/01
Posts: 6,379
Loc: orbit
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Re: Unknown bluing mushroom microscopy study [Re: EonTan]
#4685312 - 09/20/05 05:23 AM (18 years, 6 months ago) |
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i love these posts...
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 7 hours, 28 minutes
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Re: Unknown bluing mushroom microscopy study [Re: Zen Peddler]
#4798843 - 10/13/05 01:59 PM (18 years, 6 months ago) |
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Initial post updated with a couple of images.
--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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mikonn
me
Registered: 05/29/05
Posts: 192
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Re: Unknown bluing mushroom microscopy study [Re: Workman]
#4810325 - 10/16/05 09:24 AM (18 years, 6 months ago) |
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I've been finding these recently (at least I think they are the same) here in Northern Virginia. I've found them in at least 4 distinct locations over the last 3 months. Here are some pictures from a recent find.
Some displaid slight blueing, others nothing discernable. You can see some on the pin in the above pic.
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 7 hours, 28 minutes
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Re: Unknown bluing mushroom microscopy study [Re: mikonn]
#5131568 - 01/02/06 03:00 PM (18 years, 3 months ago) |
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Grown on grass seed with a small amount of horse manure added. Standard peat/calcium carbonate casing. Blues dramatically when the mushrooms abort. I am still not certain exactly what it is but it does appear to be Psilocybin active. Image on the right shows blue sclerotial like bodies growing on grass seed.
 
--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification
Edited by Workman (01/02/06 03:01 PM)
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shroomydan
exshroomerite
Registered: 07/04/04
Posts: 4,126
Loc: In the woods
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Re: Unknown bluing mushroom microscopy study [Re: Workman]
#5134476 - 01/03/06 10:28 AM (18 years, 3 months ago) |
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