rhizoRider said:

Smithii and cyanopus only differ by spore size I think also or are very similar

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Land Trout said:

Quick question on some of these finds. Are there aspen cottonwood, or other tree species that spread by long runner roots? Just wondering because I dug around where some smithii grow and found some dead runners from cottonwood that were colonized by a similar looking mycelium, the other patch in my yard has black locust runners around it. Just wondering if that could be a good key for finding and cultivating them. Just to be clear I’m not hypothesizing that they are mycorrhizal, but that they may colonized dead roots underground

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Yes, there are some aspens around nearby my finds. I don't think black locust trees occurs naturally in Finland.

A couple of late yard finds from last week.

knarkkorven said:

Alan Rockefeller said:

I see a 5% difference in the ITS sequence between JX968157 and MG871468, so if those are correctly labeled they are definitely different species, or if not, there are two species in that group.  Another unique sequence in that group turned up in Indiana and is visible via https://mycomap.com/genetics/blast-search but is not in Genbank yet.

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Yes, it would be nice to see if the mushrooms in this thread are identical to the MG871468 (my find).
If so, I guess we could rename the thread to Pholiotina smithii. I think it is.

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Quote:

Unfortunately the sequencing on both samples failed (sample 1 - ITS noisy, sample 2 - PCR failed). However, sample 1 (my yard finds) returned as 98.98% match with knarkkorvens Pholiotina smithii finds (MG871468).

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Well, the fail is noisy but it's not bad. Enough to pass the message.

My find from 13 years ago for reference
https://www.shroomery.org/forums/showflat.php/Number/14895064/fpart/4/vc/1

Looks like this when I run it through BLAST today. The top one is my find. The most identical at 99,38% was found in the state of New York.
Back when I found it, no others was that close.
Do you think yours will show up in the database eventually, or perhaps it won't be entered because of the noisy quality?

Land Trout said:

Looks like it’s recognized as Conocybula now. Make a lot of sense to me
inatrulist

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Cool! So it finally happened.
Found a paper about it.

knarkkorven said:

Well, the fail is noisy but it's not bad. Enough to pass the message.

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Conocybula sect. cyanopodae

So, the Conocybula is closer related to the Conocybe and Bolbitius than Pholiotina.

Also a new good key and descriptions from page 17 in the pdf.

Quote:

I know there are no confirmed Co. smithii finds from Finland. Pretty cool!

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And mine was the first confirmed from Sweden.

rhizoRider said:

They shud pop here in upper Wisconsin usa any day or week too. Cool 😎 plz go get more pics asap

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Good luck, I hope you'll find them! I'll try to get more photos soon for sure!

knarkkorven said:

Nice to see that this has become a summer tradition

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Yea it's pretty cool that these are not some random rare encounters anymore. The habitats I find them seem to fruit pretty consistently year after year. Ofcourse there's a still a bit more work to do about cracking the code, but hopefully we'll get there.