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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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I need experts help pan cyan issues
    #29475628 -

This is my last tray of p cyan and I cant seem to get it to grow. In a tote with 316 gph of airstones/airflow and an aquarium heater at 84 degrees F which is a bit above ambient room temps. Humidity has been around 92-95% and i keep the room a solid 80-81Β°F (ignore the 76 degrees its cause i opened the door to the room) all my other trays have failed with mold and this is my last attempt.
i dont fan or spray it

What am I doing wrong? Ive grown plenty of cubes at this point. I used a 1.5 inch depth mixture of grain to horse manure sub and it colonized perfectly fine then cased with 50/50 and some lime I just cant get this to pin :frown: tray is almost a month old at this point and no signs of contam.


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β€’ All cubensis mushrooms belong to one species therefore they're not called "strains", they're a variety of the same species :smile:

Edited by Auphex (02/06/26 05:55 PM)

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Re: I need experts help pan cyan issues [Re: Auphex]
    #29475683 -

I don't know what kind you have but your temperature seems to high. Mine have been doing great around 74 degrees. Also high humidity is good but you need some evaporation too. Maybe a little more fresh air?

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Re: I need experts help pan cyan issues [Re: CarlSagansRayGuns] * 1
    #29475711 -

Pan jams. To my understanding they thrive in 78-84ish degrees. I also found this little dot but I cant tell if its a pin.


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β€’ All cubensis mushrooms belong to one species therefore they're not called "strains", they're a variety of the same species :smile:

Edited by Auphex (02/06/26 07:06 PM)

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Re: I need experts help pan cyan issues [Re: Auphex] * 1
    #29475714 -

Auphex said:


i dont fan or spray it


The surface looks dry.  Which at this point means you probably need to scrape the casing off, recase it, and keep the humidity much higher until you see pins (98%).  That's no guarantee it will recover.

And pans don't need much fresh air tbh.  They like periodic evaporation more.  But that's when they're actually fruiting.  Right now keep the humidity high and don't worry so much about air exchange.  Also, 75f is much easier to manage.

The growth perameters from the mushroom cultivator are here, and in my opinion, they're still valid.

https://www.shroomery.org/8698/Panaeolus-cyanescens-growth-parameters


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (02/06/26 07:08 PM)

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Re: I need experts help pan cyan issues [Re: tree frog] * 1
    #29475716 -

For clarity, I think it needs to be recased because it's been sitting a very long time.  Earlier you may have been able to spray it well and raise the humidity.

You can still try that, but with how the surface looks I think the water is going to hit hydrophobic dry mycelium and suffocate the substrate.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: I need experts help pan cyan issues [Re: tree frog]
    #29475718 -

How do i go about adding more humidity? Bin is already essentially sealed with just enough space for some air through the lid. Casing is also nice n hydrated a lil wet even though i understand it looks kinda dry in photo


--------------------

β€’ All cubensis mushrooms belong to one species therefore they're not called "strains", they're a variety of the same species :smile:

Edited by Auphex (02/06/26 07:09 PM)

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Re: I need experts help pan cyan issues [Re: tree frog]
    #29475728 -

heres a clearer pic of it atm


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β€’ All cubensis mushrooms belong to one species therefore they're not called "strains", they're a variety of the same species :smile:

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Re: I need experts help pan cyan issues [Re: Auphex]
    #29475771 -

I would assume you need to decrease CO2 levels to encourage pinning and drop that temperature down if 80F+ is in fact the case. Possibly cold shock and reset conditions for fruiting.


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Edited by FounderZ (02/06/26 07:39 PM)

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Re: I need experts help pan cyan issues [Re: Auphex] * 1
    #29475827 -

Auphex said:
How do i go about adding more humidity? Bin is already essentially sealed with just enough space for some air through the lid. Casing is also nice n hydrated a lil wet even though i understand it looks kinda dry in photo


Lower the temperature to the mid 70s and the rH will go up.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: I need experts help pan cyan issues [Re: tree frog]
    #29475836 -

tree frog said:
Lower the temperature to the mid 70s and the rH will go up.


Could you explain that one please? I always thought warmer water would increase humidity


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: I need experts help pan cyan issues [Re: tree frog] * 1
    #29475838 -

And to clarify that, rH is what percentage of water air can hold before it's saturated and essentially rains.  Warmer air can hold more water.

So when the temperature is lower, the evaporation from your bubbler will create higher humidity in your chamber.

Mid 70s is good for every pan cyan strain I've run.  The higher it goes above that the more difficult it is to keep the rest of the conditions where we want them.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: I need experts help pan cyan issues [Re: PuffM4gicDr4gon] * 1
    #29475843 -

The warmer water should evaporate more in a bubbler like that, but in this situation I would first try lowering the temperature and seeing what the humidity does at 75. Like in the morning he will be able to tell if that did the trick.

If it didn't then the next option is to close the lid and maybe put a hole or two in the tote that is smaller.  Or close the lid but not snap it, or something along those lines but I reached for the simplest solution first

Especially considering I would recommend lowering the temperature anyway.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (02/06/26 09:07 PM)

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Re: I need experts help pan cyan issues [Re: tree frog]
    #29476010 -

Noted :smile: Temps lowered. Couldnt seem to get above 95% hope it helps thank you for all the help.


--------------------

β€’ All cubensis mushrooms belong to one species therefore they're not called "strains", they're a variety of the same species :smile:

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Re: I need experts help pan cyan issues [Re: tree frog] * 3
    #29476048 -

That orange bubble is bacteria.  I’d try something other than manure.  Either worm castings or go with liquid ferts via Mojave myc https://www.shroomery.org/forums/showflat.php/Number/29071244#29071244

Or a combination of both

Along with the conditions adjustments. 

I’ve had so many problems with manure when not using a spotless clean culture and/or a culture that is really aggressive like bvi.  The only manure that worked as well as cv+ or worm castings (or combo) was composted manure.  Straight field aged manure has given me so much trouble (especially with ms cultures) that I’ve totally switched it for a combination of cv+ with worm castings.  The difference is pretty crazy ime. 

I can tell within a day of spawning to straight pasteurized manure whether or not a tray is going to fruit well.  It’s how quickly the grains recover.  That’s how many shitty trays I’ve had with manure.  If it takes longer than a day for the grains to fuzz back up with myc, you’re in trouble.  If it takes longer than two, don’t even bother.. it’s a waste of casing and chamber space. 

I did an experiment where I spawned an obviously bacterial jar to cv+ with worm castings and the grains kept up the pace for recovery with a healthy tray.  The myc wasn’t as thick but it still colonized.  I cased it and kept it around.. it took 16 days to see pins.  Zero off smells the entire time.  It’s like it had a bacteria cold and eventually beat it and gave me two flushes.. this is the first


That tray is 3 weeks old.  The 2nd was even better.. it never fully opened its caps and produced spores through either flush but it grew mushrooms where a manure sub definitely would not have.. it would’ve started smelling sweet in like two days.

Some comparison pics.. first tray is a healthy sub of cv+ w/worm castings and the second pic is that bacterial tray from above (also with the same cv+ wc mix).  Both finished colonizing at the same time. 


That healthy tray was the first tray I fruited with that WC mix and it was some of the healthiest fruits I’ve grown.  They held their color, morphology was great and didn’t drop spores prematurely… also, one of the fastest trays to recover, colonize, pin, and fruit.


As a further experiment in dealing with grain recovery after spawning, I took a spawn jar that I would normally not even give a second thought about tossing out because of bacteria and I spawned it to the cv+ worm castings substrate.. here’s what the jar looked like.  The left and middle are good to go and the right is trash and the one I used for this experiment


Here it is spawned (it’s just a tester tray I never intended on fruiting)


This is 24 hours later


While it’s not full grain recovery, there’s a good amount.  In a manure sub, there wouldn’t be any.. especially after only 24 hours

I week later


Fully colonized (except for the infected grain surfaces)

No off smells.. none.

Here it is today, a month later


Some of the grain surfaces finally caught mold a few days ago and I can definitely smell it now but this also sat in a closet that whole time with an aerated lid on.

Those grains on a manure sub probably would’ve went moldy in a few days.  If you’re seeing bubbles of bacterial goo coming off your substrate surface, your sub is infected and probably the main reason you’re not seeing any flushing whatsoever.  Even tho pans are pretty finicky about the conditions that they fruit well in, you should have still seen some kind of reproductive push of that substrate in your conditions.  Something is wrong with the sub imo.

Do what you will with all that info, but I’ll never go back to pasteurized manure.

Edited by fahtster (02/06/26 10:47 PM)

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Re: I need experts help pan cyan issues [Re: fahtster]
    #29476265 -

No wonder my manure experiments have showed little to no growth! Turns out, manure sucks! :lol: my bag of manure is going straight to the garbage!

Thanks Faht! Excellent post πŸ‘πŸ™ŒπŸ‘ŒπŸ€Œ


--------------------
"A single lifetime in this density can accomplish a refinement of Will that might take far longer in a realm where the truth is always visible" - Zii, Confederation of Planets, GFL Station.

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Re: I need experts help pan cyan issues [Re: fahtster]
    #29476387 -

Wow! Thank you for the reply. This surprises me that 90%+ people even on shroomery use manure as a sub for their p. Cyans. I'll try to play around with a worm casing sub or even as you mentioned use some manure and PC worm castings in jars to mix into the sub. As for my LC I was lazy and bought pan jam syringe from sporeworks then made my own LC vials with it so idk if its just piss poor genetics from them but they colonized my grain fairly quick so I doubt it's that. So I lowered my external temps and i'll try a different substrate and see how it goes into the future. Really appreciate the time you spent into writing that. Much appreciated my dude :smile: i'll prob start with CVG and throw in a good amount of worm casing in the mix


--------------------

β€’ All cubensis mushrooms belong to one species therefore they're not called "strains", they're a variety of the same species :smile:

Edited by Auphex (02/07/26 11:07 AM)

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Re: I need experts help pan cyan issues [Re: LogicaL Chaos] * 1
    #29476406 -

LogicaL Chaos said:
No wonder my manure experiments have showed little to no growth! Turns out, manure sucks! :lol: my bag of manure is going straight to the garbage!

Thanks Faht! Excellent post πŸ‘πŸ™ŒπŸ‘ŒπŸ€Œ


Manure doesn't suck, but it is shitty!

Even using button mushroom compost was more headache than it was worth with pans.

I see some other issues with the sub and missed the bacteria photo.  But in the upper right corner of the last photo, there appears to be some spiky looking growth that maybe a different fungi species.

Anyway, if you can't get the humidity up I think you're set up can still work though that tray might be a lost cause. But you'll have to manually manage the surface conditions with a spray bottle to keep it in the Goldilocks zone (not to wet and not dry) or restrict air flow at the surface using a deeper tray or something along those lines.

I'm also a big fan of smaller containers, fewer eggs in one basket.  Food prep containers like faht is using are a great option.

But even cased half pint jars of popcorn work well for personal use.  I ran out about twenty of those recently and lost very few projects.  Plus, they go four or five good flushes.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: I need experts help pan cyan issues [Re: Auphex] * 1
    #29476426 -

Auphex said:
Wow! Thank you for the reply. This surprises me that 90%+ people even on shroomery use manure as a sub for their p. Cyans. I'll try to play around with a worm casing sub or even as you mentioned use some manure and PC worm castings in jars to mix into the sub. As for my LC I was lazy and bought pan jam syringe from sporeworks then made my own LC vials with it so idk if its just piss poor genetics from them but they colonized my grain fairly quick so I doubt it's that. So I lowered my external temps and i'll try a different substrate and see how it goes into the future. Really appreciate the time you spent into writing that. Much appreciated my dude :smile: i'll prob start with CVG and throw in a good amount of worm casing in the mix


Just a heads up that WC’s should be pasteurized.  I think there’s just such a large bio load in field aged manure that pasteurization doesn’t quite do what ppl want it to do.  WC’s doesn’t seem to have that problem.  You can sterilize manure and use it in AIO jars/bags and it’ll work great which is what a lot of pan growers do

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Re: I need experts help pan cyan issues [Re: fahtster]
    #29483145 -

Heres an update. 2 i guess massive jams & a few pins here and there i was expecting these to be a lot smaller & thinner. I started fanning it manually since the myc started getting stretchy and a week later heres the results they look like cubes but the myc says otherwise


--------------------

β€’ All cubensis mushrooms belong to one species therefore they're not called "strains", they're a variety of the same species :smile:

Edited by Auphex (02/13/26 09:55 AM)

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Re: I need experts help pan cyan issues [Re: Auphex]
    #29483171 -

I've never succeeded growing Pans so don't trust me but they look like cubes to me. Haven't seen pictures of any Pans that look like that.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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