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OfflineTravelAgency
The ongoing "wow"

Registered: 12/25/10
Posts: 4,387
Last seen: 4 months, 10 days
Great poster
11/06/18 06:20 AM

Quote:

Supalemonhaze said:
Senescene gets put on hold in cold storage. Plates can be kept for a couple of months, probably more but if you want long term storage, master slants is definitely the way to go. Also, cold storage is done in a fridge, exposed mycelium is not able to survive freezing. When  you use a piece of wood like the tek I linked, cultures are able to survive a couple of years in slants during cold storage. It is always recommended to make a new slant before 2 years though, just in case. Without the wood, slants are said to last a whole year less.

Cloning is just as easy as a spore inoculation. Just grab the best looking shroom from the biggest cluster and prep your SAB. Use your hands to manually split the shroom down the middle and rub your flame sterilized scalpel in the newly exposed tissue until you get a piece. Transfer that to a plate and treat it like any new culture, that is to make a couple of transfers to ensure cleanliness. It is actually easier to clone than to get healthy mycelium from a spore inoculation since the clone is already alive. A lot of times, molds and bacteria are able to grow before your mushroom mycelium when using spores so you have to clean them up. A clone normally grows enough to take a transfer before any molds are able to germinate. I find bacteria is more common with molds but they are easy to get rid of.

Some vendors go to extra trouble to make sure the syringes/prints they supply you are super clean yet even with a vendor bought syringe, sometimes you come across contams. Homemade prints can be rather clean but going from print to syringe is an extra step where something is bound to end up in your solution. I have yet to use a syringe straight to grains, be it vendor and homemade. I started with agar from the get-go and I am very pleased with it.




Very well stated.
OfflinePscientist
KushKaptain
Male

Registered: 11/13/09
Posts: 2,648
Loc: Gone Fission on Neuropa
Last seen: 3 years, 6 months
Good Guy
09/17/16 03:09 PM

This shroomerite is a very understanding and patient dude.
Much respect to you. :hug:

-Pscientist
InvisiblePsychedel.EXE
AKA Old Uncle Nutty
Male

Registered: 07/04/16
Posts: 211
+5
08/15/16 09:14 PM

I've noticed that you are pretty awesome. One of the good ones around here for sure. Keep up the good work.
InvisibleKenInVic
Hey Bulldog
Male User Gallery

Registered: 03/01/16
Posts: 1,452
Loc: 3rd Stone from the Sun Flag
All 'round Good
08/06/16 12:19 PM

Supportive of noobs and has good advice.  Knows this forum and its players and will sometimes guide a noob through the land mine of posting in here.  Likes to reach out to people.
OfflineMushierage
SWIM Sinker
 User Gallery

Registered: 06/29/16
Posts: 1,094
Last seen: 5 years, 1 month
:)
08/05/16 06:22 AM

Sup is an awesome guy.  Offered me the help I needed to get out of the Stone Age of cultivation, from information I learned nearly 20 years ago.  5 stars all the way.  Very knowledgable and trusted cultivator
Offlinelukehighwalker710
Cloud 9 Dweller
Male

Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 4 years, 18 days
ton of help
07/19/16 04:06 PM

Thank you for your responses to my questions, you're a helpful fella
InvisibleTheChief
Cube Collector
Male User Gallery

Registered: 07/09/14
Posts: 1,905
Loc: Executing TEKs... Flag
Good Poster
07/12/16 10:56 AM

Good info

Good 5 shrooms.

Eat up.

:chief:
OfflinePreparationH
apply daily

Registered: 03/28/05
Posts: 17,642
Loc: Amsterdam
Last seen: 7 hours, 56 minutes
4
07/03/16 01:31 AM

shrooms are better than 5 :rofl: 
Thank you though, lemme know if you want me to change this to +5.  User is a good dude.

Supalemonhaze responded on 07/07/16 04:42 AM:

Fuck the norm! 4 shrooms it is. :lol:

OfflineTmethylM
Smear in the shale
 User Gallery

Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 5 months
+5 -5 -5 +10 / 2 + 5 -5 = +5 Shrooms (of course)
06/30/16 10:22 PM

Reasons.
Several.
Reasons.
InvisibleComebackKid
Multispore Enthusiast
Male User Gallery

Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
This guy
06/25/16 10:24 PM

Knows a thing or two about a thing or two!
Thanks for clearing that up and being cool about it!
OfflineRosen_Rot
Learning
I'm a teapot

Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 1 year, 1 month
Gold
06/24/16 01:59 PM

Informative, concise and resourceful

Solid guy :sporedrop: :sporedrop: :sporedrop:
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
wtf
06/24/16 05:26 AM

you know when u see poster put so many good response out there that you thought you had rated him already because you were so used to seeing him?  thats what happened hereā€¦.my bad dude :facepalm:

seriously cool guy here.

keep it real:mushroom2:
Offlinerevmagi
Stranger

Registered: 04/09/16
Posts: 10
Last seen: 2 years, 8 months
Supalemonhaze ia a great mentor!!
06/11/16 08:28 PM

Supalemonhaze has been a huge help and mentor. He has helped me go from a failing expensive fruiting chamber to an inexpensive simple and successful fruiting chamber. He has also helped me expand from standard PF tech, to trying to hand at Agar methods using his PP5 petri method for spores swabs, and cloning.  Thanks Supalemonhaze !!!!
OfflineLt.Berkenstine
Stranger

Registered: 03/10/16
Posts: 305
Last seen: 5 years, 2 months
constantly helpful
06/05/16 09:45 AM

most helpful person here right now by far and a joy to talk to. keep it up man
OfflineEpistrophy
 User Gallery

Registered: 09/01/11
Posts: 3,014
Last seen: 22 days, 5 hours
Great guy
06/05/16 09:28 AM

This guy is very knowledgable and truly cares
OfflineBigbadwooof
Trump's Bone Spurs
 User Gallery

Registered: 12/07/13
Posts: 10,435
Last seen: 1 month, 2 days
Solid dude!
05/30/16 07:08 PM

Nice to have conversations with about boomers! Thanks buddy :smile:
Offlinespacechildo
proletarians rise up
Male User Gallery

Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 4 years, 4 months
was sure
05/28/16 06:56 PM

I rated you a long time ago :facepalm:
doing great on the boards man, you're on fire!
https://www.shroomery.org/forums/postgraph.php?userid=365228 :super:

Supalemonhaze responded on 05/29/16 02:11 AM:

Hey! I didn't know about the graphs. Cool. The part where it hits rock bottom reminds me of the time I did a couple of frontflips off my motorcycle, not fun.

OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,200
Last seen: 8 months, 24 days
Take 5! :]
05/22/16 09:09 AM

Very knowledgeable shroomerite, always posting helpful feedback, and amazing grows so far man! :woot:
OfflineAubatron
Stranger
Male

Registered: 10/24/09
Posts: 55
Loc: Canada, Halifax, NS
Last seen: 5 years, 8 months
thanks
05/20/16 07:46 PM

Nice guy, helpful link
OfflineGroo
sola dosis facit venenum
 User Gallery

Registered: 12/06/14
Posts: 2,309
Last seen: 8 months, 17 days
NICE
05/19/16 10:39 AM

Quota met https://files.shroomery.org/files/16-20/365076477-20160519_113020.jpg nice home made lid you have everywhere and pretty blue agar! Nice work!

Supalemonhaze responded on 05/21/16 03:14 PM:

I love blue agar for the easy you can see contams with when using it. You should definitely try it!

:solidnod:

OfflineJaboka
Kos... or some say Kosm?
 User Gallery

Registered: 10/28/14
Posts: 295
Loc: IC 1101
Last seen: 2 days, 21 hours
Really helpful
05/18/16 04:25 AM

This dude knows his stuff. Keep informing the noobies! We need people like you.
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery

Registered: 07/16/12
Posts: 18,916
haazzeee
05/18/16 01:52 AM

I like ya dude keep on rockin' it  :headbang:
Invisiblethe_r3dz
Trich Propagator
 User Gallery

Registered: 03/26/15
Posts: 5,709
Right on dude
05/17/16 06:38 PM

Keep being awesome
InvisibleMunchauzen
 User Gallery

Registered: 06/23/11
Posts: 14,267
5 for you
05/17/16 11:30 AM

:thumbup:
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery

Registered: 04/30/13
Posts: 61,794
Loc: Milky way
oops missed this one
05/14/16 11:52 PM

better late than never,

also camp is a loser
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