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InvisibleZen Peddler
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Registered: 06/19/01
Posts: 6,379
Loc: orbit
microwave sterilisation
    #345751 - 06/21/01 11:46 AM (21 years, 3 months ago)

Does anyone have any info on sterilising using a microwave for jars of 250ml water and 250ml/500ml verm. substrates?? Ive done it with water for eight minutes - is this enough, and with the substrate, it explodes all the time - any suggestions?



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Offlinemadscientist
journeyman
Registered: 05/26/01
Posts: 110
Last seen: 20 years, 2 months
Re: microwave sterilisation [Re: Zen Peddler]
    #345769 - 06/21/01 01:09 PM (21 years, 3 months ago)

Stck to pressure cooking. Im a microbiologist and I once microwaved a flask of nutrient broth for 12 minutes and within 2 days I could see bacterial contaminants. Better to avoid any chance of contamination not just to avoid losing jars but because contaminats can put you in the hospital and/or give you cancer.



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Instead of the dove as the symbol of peace we should have a pillow. Its got more feathers but doesnt have that nasty sharp beak......


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InvisibleCannonE
Stranger
Registered: 05/06/01
Posts: 15
Re: microwave sterilisation [Re: madscientist]
    #345784 - 06/21/01 02:12 PM (21 years, 3 months ago)

Put you in the hospital....ok I've seen this one. But give you cancer? On what basis do you make this statement? Infectious forms of cancer are caused by Hepatitis viruses, HPV, but I've not heard of any fungus causing cancer.



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Offlinemadscientist
journeyman
Registered: 05/26/01
Posts: 110
Last seen: 20 years, 2 months
Re: microwave sterilisation [Re: CannonE]
    #345795 - 06/21/01 03:06 PM (21 years, 3 months ago)

Carcinogens would come from fungal contaminants. Many common fungi produce a class of toxins known as 'aphlatoxins'. These are not only toxic in the traditional sense but when consumed in small amounts repeatedly they can cause cancer of the oesophagus and mouth (aphlatoxins are often present in peanut butter). In some areas of South Africa, contamination of maize meal by fungi producing aphlatoxins has resulted in locally elevated levels of oesophageal cancer making it the most prevalent form of cancer after cervical cancer ( HPV in woman only) in these areas.




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Instead of the dove as the symbol of peace we should have a pillow. Its got more feathers but doesnt have that nasty sharp beak......


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OfflineNagual
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Registered: 06/08/01
Posts: 316
Loc: nyc, USA
Last seen: 18 years, 9 months
Re: microwave sterilisation [Re: madscientist]
    #345799 - 06/21/01 03:19 PM (21 years, 3 months ago)

The madscientist is scaaaaring meeeee..
So what does this 'mean'? Should I cut peanut butter (mmmmmm
pnut butter) corn and mushrooms out of my diet or simply just
don't eat anything with a slimy green growth hanging off of it?
Also do you mean ALPHAtoxin? Thanks for looking out for our
heath man.
o0O)}>I seem to be a verb<{(O0o
the FSR is groovalicious
!!Crobih is my founder of the Shroomery!!

Edited by Nagual on 06/21/01 08:22 AM.



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InvisibleCannonE
Stranger
Registered: 05/06/01
Posts: 15
Re: microwave sterilisation [Re: madscientist]
    #346060 - 06/22/01 12:47 AM (21 years, 3 months ago)

Right...sorry I forgot about Aspergillus, and it's pesky liver ca causing aflatoxin. (Micro was a couple of years ago). Thanks for clearing that up. I'm not able to access my internet resources from home, so I'll ask you this out of convenience....is aflatoxin heat labile? Just wondering if making tea helps prevent exposure....

Thanks



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Offlinemadscientist
journeyman
Registered: 05/26/01
Posts: 110
Last seen: 20 years, 2 months
Re: microwave sterilisation [Re: CannonE]
    #346450 - 06/22/01 10:46 AM (21 years, 3 months ago)

Ermmm... I must be honest and say that i dunno about heat stability of aflatoxins. I normally dont get any nausea from tea though so it prolly does help to denature some nasties. I'll see if I can find out though... tis an interesting question.



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Instead of the dove as the symbol of peace we should have a pillow. Its got more feathers but doesnt have that nasty sharp beak......


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Offlinemadscientist
journeyman
Registered: 05/26/01
Posts: 110
Last seen: 20 years, 2 months
Re: microwave sterilisation [Re: Nagual]
    #346455 - 06/22/01 10:54 AM (21 years, 3 months ago)

Nope, tis aphla(or afla?)toxins. pnut butter is prolly the most oft contaminated foodstuff but considering how much is eaten on a daily basis and how few cases of poisoning are actually reported I guess its pretty safe, depends on how much you dig the stuff! (i hate it). Dont eat anything with mould onit.



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Instead of the dove as the symbol of peace we should have a pillow. Its got more feathers but doesnt have that nasty sharp beak......


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OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,148
Loc: my room
Last seen: 2 months, 1 day
Re: microwave sterilisation [Re: madscientist]
    #346469 - 06/22/01 11:19 AM (21 years, 3 months ago)

yes, it is heat-stable.
It?s spelled aFLatoxin.

Read
http://www.ext.colostate.edu/pubs/crops/00306.html

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InvisibleCannonE
Stranger
Registered: 05/06/01
Posts: 15
Re: microwave sterilisation [Re: Anno]
    #346529 - 06/22/01 04:18 PM (21 years, 3 months ago)

This is concerning.....I also did a pub-med seach, and it seems to survive (at least in part) microwaving and boiling. Doesnt' sound great for our livers. Hopefully since you don't eat this stuff daily, and it is in much reduced quantities than seen in 3rd world countries, we'll be OK.



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