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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: A Trip to The Cabin: Easy to Follow Pictorial TEK For Cabin Sequestration [Re: catoblepa] * 5
    #29474018 -

The halo is normal in my opinion it’s just enzymes breaking down the sugars/agar, unless the halo is wet and cloudy looking and on the surface then you probably have bacteria, but a transparent halo that’s through the depth of the agar should be normal


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Re: A Trip to The Cabin: Easy to Follow Pictorial TEK For Cabin Sequestration [Re: 7Suns]
    #29474272 -

Thank you so much for the reply 7Suns. I'm gonna let this grow out some more and transfer it to grain and/or another plate, same for the other samples inside the cabins :smile:


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Garbage in, garbage out.

Life saver for contamination (cabin sequestering):
https://www.youtube.com/watch?v=rhwoNYmu_LA

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Re: A Trip to The Cabin: Easy to Follow Pictorial TEK For Cabin Sequestration [Re: Siphonophorest] * 1
    #29514459 -

This is awesome great tek and explanation this needs to be pinned or bumped especially for newbies like me this is great ty

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Re: A Trip to The Cabin: Easy to Follow Pictorial TEK For Cabin Sequestration [Re: Torfield] * 3
    #29514492 -

A lot of folks are diggin the cabin. If you think it's worth sharing, consider adding the link to your signature :sunny:


:mushroom2: Welcome to the Shroomery :mushroom2:


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MUSHROOMS RULE

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Re: A Trip to The Cabin: Easy to Follow Pictorial TEK For Cabin Sequestration [Re: Dala] * 2
    #29640222 -

Excited to try this out for the first time.

Very inspiring thread, thanks everyone for the info. I used this glass cutter tool and it works great for microscope slides.

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Re: A Trip to The Cabin: Easy to Follow Pictorial TEK For Cabin Sequestration [Re: ramthroughram]
    #29640276 -

ramthroughram said:
Excited to try this out for the first time.

Very inspiring thread, thanks everyone for the info. I used this glass cutter tool and it works great for microscope slides.


:nice:


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: A Trip to The Cabin: Easy to Follow Pictorial TEK For Cabin Sequestration [Re: Kloakk]
    #29695018 -

Thoughts on easiest way to sterilise a few glass slides? Are you using wet or dry heat (oven or pressure cooker)?

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Re: A Trip to The Cabin: Easy to Follow Pictorial TEK For Cabin Sequestration [Re: bongoman] * 2
    #29695221 -

bongoman said:
Thoughts on easiest way to sterilise a few glass slides? Are you using wet or dry heat (oven or pressure cooker)?


I pack them in foil and inside a PP5 container when I sterilize agar. I'm sure you could use dry heat, but haven't done it myself.



--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: A Trip to The Cabin: Easy to Follow Pictorial TEK For Cabin Sequestration [Re: bongoman] * 2
    #29695273 -

bongoman said:
Thoughts on easiest way to sterilise a few glass slides? Are you using wet or dry heat (oven or pressure cooker)?


I dry heat sterilize my glass petris and I add my slides to one of those empty dishes.



--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: A Trip to The Cabin: Easy to Follow Pictorial TEK For Cabin Sequestration [Re: bongoman]
    #29695438 -

bongoman said:
Thoughts on easiest way to sterilise a few glass slides? Are you using wet or dry heat (oven or pressure cooker)?


If you haven’t planned ahead well enough to PC your slides as Kloakk and Scemilope suggest, I’ve had pretty good success dipping in IPA and flame sterilising. This DOES NOT work with cover slips which begin to melt and will bend. PCing is the best approach for both slides and cover slips but if you’re in a pinch flaming works for slides.

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Re: A Trip to The Cabin: Easy to Follow Pictorial TEK For Cabin Sequestration [Re: MomoMyco] * 1
    #29695537 -

I have a strict protocol to only open brand new packs of slide and slips in the hood and then reseal with the impulse sealer each time and I pull from the stack with flamed tweezers so the packaging stays sterile after every use, it’s more steps but then I don’t have to PC it


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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