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Volvariella volvacea or mold?
    #12354488 -

Hi,

got another strange mycelium, which differs from everything I've seen before. Unfortunately I can't find any closeup images showing mycelium, so those of you who are growing paddy straw mushrooms, please have a look at the images below and tell me what you think.

Thanks, Carsten


On agar:


On straw:



On compost:



Crawling up the glass wall:

Edited by Mycelio (04/08/10 10:52 AM)

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Re: Volvariella volvacea or mold? [Re: Mycelio]
    #12354653 -

Looks like cobweb to me


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             :crazykitty:

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Re: Volvariella volvacea or mold? [Re: ninja cat 09]
    #12355381 -

Sure, but would mycelium of Vv look different?

Carsten

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Re: Volvariella volvacea or mold? [Re: Mycelio]
    #12355575 -

I have never seen volvariella mycelium, but I'd eat my hat if that doesn't turn out to be cobweb mold.

/edit: looking at houdinihar's thread on this species, I think I might head down to the store and see if they carry some tasty hats.

Edited by koraks (04/08/10 01:53 PM)

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Re: Volvariella volvacea or mold? [Re: koraks]
    #12355757 -

^ what these guys said, looks like cobweb.  It will take over the entire substrate within a day, should be obvious.

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Re: Volvariella volvacea or mold? [Re: Mycelio] * 1
    #12355938 -

Hey Mycelio,

I saw this flipping through the GGMM and I noticed that the Volvariella mycelium looks very much like this on agar. I know cobweb looks like that on substrates (but tighter) but it seemed that cobweb was more tightly knitted compared to that of the Volvariella where it seems extremely spread out and very airey. I think you got a good chance its clean but looks very close to cobweb.

Like the first time you see Pleurotus cystidious with all its little black spots all over the agar and grain. Just downright creepy.


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Master Slants Made Simple

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Re: Volvariella volvacea or mold? [Re: Mephistophelian]
    #12356247 -

Thanks everybody for trying to help (and bon appetit to Koraks :grin:).

Mephistophelian, I'm glad you chimed in with some experience. Knowing that it grows similar to cobweb helps a lot. Unfortunately I have never seen cobweb myself. Perhaps it's also a good sign that it doesn't spread so fast at 19°C, but speeds up at 24°C. Let's see how far I get with this one.

Carsten

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Re: Volvariella volvacea or mold? [Re: Mycelio]
    #12356733 -

Well I've had cobweb before and it takes no more then 3-4 days to totally fill a jar of grain.

I haven't personally grown Paddy Straw but I remember the picture and its also a very hot weathered mushroom, so high speed colonizing at 24 makes sense where its very dormant at 19. Especially that it will die if refrigerated. :thumbup:


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Master Slants Made Simple

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Re: Volvariella volvacea or mold? [Re: Mycelio]
    #12356803 -

All pictures are same dish at 3 days. The leading edge of the mycelium is very fine. Quarter inch back from the leading edge the mycelium start to get aerial with a curling nature. I just transferred a few wedges to some grain last night. I will post pics when I get around to it in a few days. Do you have a microscope mycelio? Shouldn't be hard to tell Paddy straw from cobweb under the scope.



Lipa

Edited by lipa (04/08/10 05:37 PM)

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Re: Volvariella volvacea or mold? [Re: Mephistophelian]
    #12356938 -

Mephistophelian said:
Well I've had cobweb before and it takes no more then 3-4 days to totally fill a jar of grain.

I haven't personally grown Paddy Straw but I remember the picture and its also a very hot weathered mushroom, so high speed colonizing at 24 makes sense where its very dormant at 19. Especially that it will die if refrigerated. :thumbup:


Oh, I thought you did grow them before. Must have mixed it up, I better start taking hericium pills. :laugh:

Carsten

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Re: Volvariella volvacea or mold? [Re: Mycelio]
    #12357141 -

I will when I get a culture. Mine died last year :frown:

I'm just going on memory of the pictures, hmmmm...maybe that hericium helped last night :wink:


--------------------
Master Slants Made Simple

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Re: Volvariella volvacea or mold? [Re: lipa]
    #12357302 -

lipa said:
All pictures are same dish at 3 days. The leading edge of the mycelium is very fine. Quarter inch back from the leading edge the mycelium start to get aerial with a curling nature. I just transferred a few wedges to some grain last night. I will post pics when I get around to it in a few days. Do you have a microscope mycelio? Shouldn't be hard to tell Paddy straw from cobweb under the scope.



Lipa


Thank you for posting those images Lipa! The mycelium on the dish I received is less curly, but it was a multispore inoculation, where it might take a while until it shows its usual shape.
Would be great, if you could post an image of your mycelium on grain in the coming days. If it doesn't form long and straight hyphae, when leaving the slants, I can be sure to have cultivated a contam. The only microscope I have access to, seems to be useless.

Carsten

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Re: Volvariella volvacea or mold? [Re: Mycelio]
    #12357534 -

If I get time tonight I will try to snap some shots under the scope for everyone to see.  This culture by the way is the 3 transfer (S-3) from a multi spore streak for those who don't know. The species seems to grow at random on the plate so I won't know how it will look as a mono culture until I clone a fruit and get a better idea. I doubt it will look much different from what I have seen so far. Being that this mushroom can tolerate very high Co2 levels I will probably try an in-vitro grow in a gallon mason first before I proceed to a mound culture. I live in the desert so it should grow pretty well here. I am going to follow procedures from the mushroom growers handbook first to get a feel for now.


A friend who lives on the big island sent these to me. I heard they were very large fruit bodies (the print was huge!!) on his compost pile. They said they were delicious.
Lipa

Edited by lipa (04/08/10 07:35 PM)

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Re: Volvariella volvacea or mold? [Re: lipa]
    #12359371 -

When I grew them the mycelium didn't seem to be as cottony as yours. But the mycelium always turned a shade of orange/orangeish tan.

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Re: Volvariella volvacea or mold? [Re: FNFAL]
    #12359470 -

This is a post by Anno in 2003

http://www.shroomery.org/forums/showflat.php/Number/1415640#1415640

Found this in his gallery

Edited by lipa (04/09/10 01:06 AM)

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Re: Volvariella volvacea or mold? [Re: lipa]
    #12362831 -

Alright, I think I found cobweb in one of my worm boxes, this is like 5 times as large, ten times as fast, has much stronger hyphae and a strong moldy smell. Scary.
Then I opened one of my tiny colonized jars. There was only the sweet sugary smell of straw pellets with bran, hurted mycelium had a mushroomy smell, but not strong. Tried using that crappy microscope, but couldn't find any christmas trees with spores, like I expected to see from cobweb, only long hyphae.

I guess I keep it as an unknown contamination, though I will wait and see if the mycelium on the dish gets more curly with time and if it changes color in one of the jars.

Thanks again to everybody for advice, links and images!

Carsten

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Re: Volvariella volvacea or mold? [Re: Mycelio]
    #12366583 -

I have had some experience with Volveriella volvacae mycelium and it is extremely fast growing, faster than any mushroom I have seen and cobwebby like that, Volveriella bombycina is extremely cobwebby but has few individual strands. I think there is a good chance that the jars are Vv mycelium .

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Re: Volvariella volvacea or mold? [Re: Lennybernadino]
    #12367617 -

Wow, this thread is full of turns. :laugh:

Thanks Lenny! Perhaps I should inoculate a straw bag and try to fruit it.

Carsten

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Re: Volvariella volvacea or mold? [Re: Mycelio]
    #12378183 -

Carsten that looks like paddy straw to me, it will tend to darken with age and turn yellow.

If it stays white then it is more likely to be a contam.


--------------------
One of these days all the answers will be revealed until then we learn from each other!

www.mushrush.co.za

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Re: Volvariella volvacea or mold? [Re: solumvita]
    #12379414 -

Hi Solumvita, that's great! I'm already inspecting my jars, looking for a color change towards yellow/orange/brown. This must be connected to the formation of asexual spores I read about.

Carsten

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Re: Volvariella volvacea or mold? [Re: Mycelio]
    #12448762 -

A little update...

The mycelium on agar didn't change its appearance much, but due to different nutrients, temperature and being the first plate after spore germination I am not worried.

In the jars, I see hyphal aggregates, turning yellow orange and then brown.

Carsten



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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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