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Liquid Inoculant (LI)
Similar to LC in that mycelium is suspended in liquid, however LI uses sterilized water and no nutrients. Mycelium is introduced into the sterilized water then agitated by blending or shaking. This mycelium laden water is then used as an inoculant
Currently, there are two common methods for producing LI. One involves the use of a blender blade attachment to blend an agar dish into the water. The other method involves adding the agar plate to a jar of water and shaking it to blend the agar into the water.
Performance wise, there is very little difference, but thats not to say there isn't one. LI made using the blender will achieve a smaller particle size and thus more inoculation points. Shaken LI will tend to have larger pieces but is easier to produce for beginners. The Blended LI may reach 100% 1-2 days faster than the Shaken LI.
LI is a good method for beginners and experienced users alike. Beginners can post pictures of plates on the shroomery to have veterans help identify clean plates. With a clean plate selected, these fairly simple and short techniques have a very high success rate - so long as you follow the tek!
FAQ:
Q: Can I store LI:
A: Yes, but the shorter the better! pasty plates only take 2 weeks to grow out, so don't be lazy.
Q: Can I use LI to inoculant a bulk substrate and skip grains?
A: Only with the bottle tek is such a thing possible. Simply pouring LI in a monotub full of CVG will not work.
Q: How long does it take to colonize grains or bottles?
A: Jars inoculated with LI will typically reach 100% colonization in 1-2 weeks.
Q: Did I ruin my jars by pouring too much LI?
A: Definitely not! Rarely does this cause an issue, but rather explosive growth. Some users have seen 100% colonization in as little as 4-5 days having used large volumes of LI.
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