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Viewing mushpunx's Ratings (100 General: 5.0 | 3 Trade: 5.0 ) Display: [ All | General | Trade ]  Sort: [ Rating | User | Date ]
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Invisibleeatyualive
Eat's You Alive :)
 User Gallery

Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
badass grows!
05/25/15 09:30 PM

nice work! i like you man. your on it and you try it all. hey, by punk do you like punk rock music?

Last Edited 01/27/16 10:10 PM
OfflineEpistrophy
 User Gallery

Registered: 09/01/11
Posts: 3,031
Last seen: 1 day, 10 hours
Really good guy
01/13/15 04:31 PM

Always like your posts and comments :thumbup:
InvisibleFantastic Mr. Fox
Grass Seed Connoisseur

Registered: 02/12/15
Posts: 3,227
Putting in workk
05/17/15 06:25 AM

:paradissunsmall:
OfflineFeedYourMind
indiGlo
 User Gallery

Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 3 days, 7 hours
Basics
03/17/15 12:28 AM

For helping me to learn the difference between the flame and ISO alcohol and their purposes! Thanks!
InvisibleFriedEggS
I'm a teapot

Registered: 09/22/14
Posts: 2,537
Loc: Taiwan
knows his shit
05/28/15 07:10 PM

you da man

smart, great grows, constantly helping people in the forums, humble.

:kimjong:

edit: i love your lights
https://www.shroomery.org/forums/showflat.php/Number/24264629#24264629

Last Edited 04/29/17 12:34 PM
Offlinegermanauslander
Forever Learner

Registered: 11/06/16
Posts: 100
Last seen: 5 years, 10 months
good advice
02/13/17 03:52 PM

Re: Re: Agar culture from spore prints

thanks man
Offlinegizmodo
Woodland Creature
Male

Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 3 years, 16 days
+5
03/02/19 09:07 AM

:pipesmoke:
Invisiblegrainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
Nice work
04/02/15 05:03 PM

Re: Re: Isolate with me

Enjoying watching you isolate.  :copypasta::willienelson:
OfflineGreenRabbit
Plutonium Pollinator
Male

Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 7 months
Its seems I haven't rated you yet.
04/26/16 10:12 AM

Lets fix that.
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
InvisibleGrey
⇜ ✯ ⇝
 User Gallery

Registered: 11/06/14
Posts: 6,223
your a nice guy....
03/09/15 09:00 PM

....inspirational and shit. Thanks for the help.
OfflineGroo
sola dosis facit venenum
 User Gallery

Registered: 12/06/14
Posts: 2,310
Last seen: 2 months, 26 days
Solid shroomer
02/18/16 09:45 PM

Down to the mycellium.
Invisiblehamloaf
Pork Block ®©™√
Male User Gallery

Registered: 12/23/09
Posts: 21,756
Loc: ation undisclosed.
+
01/03/15 05:25 AM

Good Hand.
InvisibleHtaeh
Immortal
Male

Registered: 10/14/18
Posts: 320
Really appreciated this.
05/07/20 06:48 AM

Quote:

mushpunx said:
It's just to make sure the culture is clean - even if the germination plate looks clean oftentimes the myc has germinated over top or next to bacteria or mold colonies. So make a transfer to another plate and use that to inoculate your grains. Often I'll do two transfers to be safe.
Whenever you make a transfer, you are probably cutting out at least some of the genetics, but it doesn't matter. You are just looking for a clean MS culture to inoculate grains with.
In a culture germinated from spores there are hundreds to thousands of strains present. If I am isolating strains it typically takes me 8 to 10 transfers before I see obvious sectoring. Then once I take a transfer from each sector to a new plate, those cultures might still show some sectors and I might still have to make another transfer or two of each of the next plates until they become single sector isolated strains. At that point, I'll have 10 - 20 different strains, which I label, fruit and test for potency + growing factors. There might only be one or two good ones, if I'm lucky.
Strain isolation takes a lot of work and patience!
It makes more sense for us home cultivators to clone. That is still quite a bit of work (and eating a lot of mushrooms if you wanna test potency 😂), taking lots of clones and fruiting them out, searching for potent, high yielding fruiters.


OfflineI Like Alkaloids
Brought Back to Life
Male

Registered: 05/14/07
Posts: 516
Loc: Rocky Mountains, U.S. Flag
Last seen: 8 years, 3 months
5
01/15/16 02:19 PM

Keep up the good work!
:cool:
OfflineIcon
Bloomer
 User Gallery

Registered: 05/15/14
Posts: 2,924
Last seen: 9 hours, 4 minutes
Thanks for helping pave the way
03/01/15 04:24 AM

I always learn from your questions and input. Wishing you success!
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Registered: 11/21/11
Posts: 24,867
Loc: ★
Good poster
04/04/15 12:38 AM

Dude knows what he's talking about, gives good advice wherever I see him posting.  :thumbup:
InvisibleJacobStorm
psychedelic cartel
I'm a teapot

Registered: 07/21/15
Posts: 1,499
Flow hood
08/08/16 06:17 PM

Kick ass help man thanks a grip!
Invisiblejbaby007S
Badass
I'm a teapot

Registered: 02/28/15
Posts: 1,030
Tek Time
10/04/15 05:27 PM

Sweet write up man. I have the balls to try straw, poo, and pan cyans now because your tek makes it look so easy. :heart:
Offlinejcbowling1985
J$
Male User Gallery

Registered: 07/10/14
Posts: 157
Loc: NE USA
Last seen: 7 years, 6 months
Thanks for your comment
07/16/14 06:10 PM

I'm happy you said "I just read this whole thread and it rules."

It tells me that people do really appreciate what I'm doing and I'm happy others like you love a thread like this.
Hopefully I can share some good fruits with you one day too lol...
InvisibleJean-guy Masta
Railyard Ghost
Male

Registered: 09/23/14
Posts: 1,827
Loc: MT-Hell
Crusty punk brother
03/08/15 06:11 PM

:rockon:
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