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Socially distanced, always

Registered: 10/14/18
Posts: 319
Really appreciated this.
05/07/20 08:48 AM


mushpunx said:
It's just to make sure the culture is clean - even if the germination plate looks clean oftentimes the myc has germinated over top or next to bacteria or mold colonies. So make a transfer to another plate and use that to inoculate your grains. Often I'll do two transfers to be safe.
Whenever you make a transfer, you are probably cutting out at least some of the genetics, but it doesn't matter. You are just looking for a clean MS culture to inoculate grains with.
In a culture germinated from spores there are hundreds to thousands of strains present. If I am isolating strains it typically takes me 8 to 10 transfers before I see obvious sectoring. Then once I take a transfer from each sector to a new plate, those cultures might still show some sectors and I might still have to make another transfer or two of each of the next plates until they become single sector isolated strains. At that point, I'll have 10 - 20 different strains, which I label, fruit and test for potency + growing factors. There might only be one or two good ones, if I'm lucky.
Strain isolation takes a lot of work and patience!
It makes more sense for us home cultivators to clone. That is still quite a bit of work (and eating a lot of mushrooms if you wanna test potency πŸ˜‚), taking lots of clones and fruiting them out, searching for potent, high yielding fruiters.

OfflineMagic Mushpoon
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Registered: 03/24/19
Posts: 104
Last seen: 4 months, 3 days
Good Advice
04/06/20 02:55 PM

Super helpful advice and experiences in a bunch of different threads
Woodland Creature

Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 1 month, 16 days
03/02/19 11:07 AM

InvisibleJohnny Dont
500 Don'ts of Knife Safety

Registered: 04/24/14
Posts: 1,830
Helpful and polite
02/14/19 06:11 PM

Thanks for the advice
Second Rate Mycologist
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Registered: 08/18/09
Posts: 2,194
Loc: Between a Rock and a Hard...
Last seen: 3 months, 9 days
Good info
02/12/19 07:21 PM

My nose dont work neither lol
Salty Sailor
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Registered: 02/20/14
Posts: 754
Loc: Norway Flag
Last seen: 2 months, 30 days
Peace of mind
02/08/19 07:41 PM

Male User Gallery

Registered: 12/30/16
Posts: 6,802
Loc: PNW
5 shrooms
01/31/19 10:47 PM

Nice seeing you around.  Always dropping good knowledge
Hi mom!

Registered: 05/01/16
Posts: 108
Loc: Night City 2020
Last seen: 2 years, 2 months
Thanks for the help!
01/21/19 10:47 AM

Thanks for helping with my flow hood questions!
It’s OK!! I’m a Limo Driver!! 😁
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Registered: 07/01/17
Posts: 5,712
Last seen: 29 minutes, 35 seconds
10/16/18 07:59 PM

So glad to see you back in cult again!! You're one of the best on here!
Male User Gallery

Registered: 06/10/12
Posts: 428
Loc: New York
Last seen: 4 months, 23 days
Incredible patience, kudos!
08/11/18 11:52 PM

Tote-tally awesome
I'm a teapot User Gallery

Registered: 12/18/14
Posts: 2,038
Loc: PNW
02/08/18 03:17 AM

5 more mush for da punx
Post whore
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Registered: 02/17/17
Posts: 4,316
Loc: Flag
08/12/17 09:15 AM

Smart man. Good man.

Registered: 07/26/15
Posts: 1,135
Loc: City Vanity
Last seen: 9 months, 21 days
08/05/17 01:51 PM

Where is your tc tag at? Thanks for the blower sizing for a 2x2 flow hood info in that one thread.
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Registered: 07/06/11
Posts: 735
Loc: Australia Flag
Shares info willingly
05/14/17 07:19 AM

I appreciate your help and info. Thank you
Male User Gallery

Registered: 09/12/16
Posts: 310
Loc: Canada Flag
Last seen: 3 months, 25 days
thanks man
04/09/17 10:47 PM

the strength is strong in this one.

real informative
The Fool on the Hill
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Registered: 06/18/15
Posts: 393
Loc: North Flag
Last seen: 16 days, 18 hours
Always Helpful
04/04/17 10:43 AM

Man, I can tell you know your shit. Thats why I trust your suggestions. Great pics in the signature!
 User Gallery

Registered: 03/02/17
Posts: 181
Last seen: 3 years, 11 months
Always helpful
03/31/17 05:54 PM

Thanks for the in depth responses man, you are a big help to me and many others. Always respectful.
 User Gallery

Registered: 12/03/13
Posts: 433
Loc: Maxico ☼ Flag
Last seen: 6 days, 11 hours
03/30/17 09:58 AM

:crankey: attitude
Fletcher Detcher

Registered: 02/13/14
Posts: 2,662
Loc: Chicago Flag
I havent given you one yet?
03/10/17 12:22 PM

Lets fix that!

The grow in your sig is awesome, always love seeing that canopy :grin:

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Registered: 07/23/16
Posts: 6,823
Loc: Interdimensional Bootcamp
03/08/17 09:35 AM

Nam Sayin
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Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 2 years, 7 months
02/28/17 10:21 PM

Contributions and pics!  You know what you're doing.
InvisibleThe Patient

Registered: 03/06/13
Posts: 528
02/20/17 10:41 PM

Wow fantastic pics man, gave me some inspiration for a future project. :mushroom2:
Forever Learner

Registered: 11/06/16
Posts: 100
Last seen: 2 years, 11 months
good advice
02/13/17 05:52 PM

Re: Re: Agar culture from spore prints

thanks man
The Transcendent

Registered: 06/17/12
Posts: 399
02/03/17 10:41 PM

good info on identifying sectors!
Weirdo loser
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Registered: 12/23/15
Posts: 124
Last seen: 3 years, 9 months
Those AA+ are sexy as hell
09/02/16 09:19 PM

You are killing it, love those AA+ genes you got going on. Jelly as fuck over here. Keep up the good work! you rule
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