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OfflineB H O
Male

Registered: 03/18/13
Posts: 675
Last seen: 1 month, 13 days
Solid TC!
07/01/21 04:28 AM

Thanks for answering my questions via PM
InvisibleLenz
Misunderestimated
I'm a teapot User Gallery

Registered: 08/17/20
Posts: 692
Loc: Fungistan
Super friendly and willing to help
06/28/21 08:05 PM

Appreciated the effort you gave helping me figure out my lid and filter woes. Really much appreciated and was a huge help, appreciate the generosity with your time and knowledge. :grin:
InvisibleHtaeh
Immortal
Male

Registered: 10/14/18
Posts: 320
Really appreciated this.
05/07/20 03:48 PM

Quote:

mushpunx said:
It's just to make sure the culture is clean - even if the germination plate looks clean oftentimes the myc has germinated over top or next to bacteria or mold colonies. So make a transfer to another plate and use that to inoculate your grains. Often I'll do two transfers to be safe.
Whenever you make a transfer, you are probably cutting out at least some of the genetics, but it doesn't matter. You are just looking for a clean MS culture to inoculate grains with.
In a culture germinated from spores there are hundreds to thousands of strains present. If I am isolating strains it typically takes me 8 to 10 transfers before I see obvious sectoring. Then once I take a transfer from each sector to a new plate, those cultures might still show some sectors and I might still have to make another transfer or two of each of the next plates until they become single sector isolated strains. At that point, I'll have 10 - 20 different strains, which I label, fruit and test for potency + growing factors. There might only be one or two good ones, if I'm lucky.
Strain isolation takes a lot of work and patience!
It makes more sense for us home cultivators to clone. That is still quite a bit of work (and eating a lot of mushrooms if you wanna test potency πŸ˜‚), taking lots of clones and fruiting them out, searching for potent, high yielding fruiters.


OfflineMagic Mushpoon
Stranger
 User Gallery

Registered: 03/24/19
Posts: 104
Last seen: 1 year, 7 months
Good Advice
04/06/20 09:55 PM

Super helpful advice and experiences in a bunch of different threads
Offlinegizmodo
Woodland Creature
Male

Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 1 year, 4 months
+5
03/02/19 06:07 PM

:pipesmoke:
InvisibleJohnny Dont
500 Don'ts of Knife Safety

Registered: 04/24/14
Posts: 1,830
Helpful and polite
02/15/19 01:11 AM

Thanks for the advice
OfflineDionili
Second Rate Mycologist
Male User Gallery

Registered: 08/18/09
Posts: 2,194
Loc: Between a Rock and a Hard...
Last seen: 1 year, 6 months
Good info
02/13/19 02:21 AM

My nose dont work neither lol
OfflineBlazeGlassDabs
Salty Sailor
 User Gallery

Registered: 02/20/14
Posts: 754
Loc: Norway Flag
Last seen: 1 year, 6 months
Peace of mind
02/09/19 02:41 AM

πŸ–’
InvisibleJust_A_Noob
Breathing
Male User Gallery

Registered: 12/31/16
Posts: 6,809
Loc: PNW
5 shrooms
02/01/19 05:47 AM

Nice seeing you around.  Always dropping good knowledge
OfflineJesh
Hi mom!
Male

Registered: 05/01/16
Posts: 108
Loc: Night City 2020
Last seen: 3 years, 5 months
Thanks for the help!
01/21/19 05:47 PM

Thanks for helping with my flow hood questions!
InvisibleTormatoMFacebookDiscordReddit
It’s OK!! I’m a Limo Driver!! 😁
Male User Gallery

Registered: 07/01/17
Posts: 5,910
+5
10/17/18 02:59 AM

So glad to see you back in cult again!! You're one of the best on here!
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
Offlineblthirteenre
Male User Gallery

Registered: 06/10/12
Posts: 428
Loc: New York
Last seen: 5 months, 1 day
Incredible patience, kudos!
08/12/18 06:52 AM

https://www.shroomery.org/forums/showflat.php/Number/25374586#25374586
InvisibleAyePlus
Tote-tally awesome
I'm a teapot User Gallery

Registered: 12/19/14
Posts: 2,038
Loc: PNW
5
02/08/18 10:17 AM

5 more mush for da punx
InvisibleJHOVA
Post whore
Male User Gallery

Registered: 02/18/17
Posts: 4,321
Loc: Flag
Knowledgeable
08/12/17 04:15 PM

Smart man. Good man.
Offlinewtfcrazymofo
Zym.zymo-fo
Other

Registered: 07/26/15
Posts: 1,136
Loc: City Vanity
Last seen: 2 months, 4 days
5
08/05/17 08:51 PM

Where is your tc tag at? Thanks for the blower sizing for a 2x2 flow hood info in that one thread.
InvisiblemistamonstaS
Threadkiller
I'm a teapot User Gallery

Registered: 07/06/11
Posts: 888
Loc: Uranus
Shares info willingly
05/14/17 02:19 PM

I appreciate your help and info. Thank you
OfflineMartinsapin
no-mad
Male User Gallery

Registered: 09/13/16
Posts: 310
Loc: Canada Flag
Last seen: 3 months, 30 days
thanks man
04/10/17 05:47 AM

the strength is strong in this one.

real informative
OfflinePurpleHaz
The Fool on the Hill
Male User Gallery

Registered: 06/18/15
Posts: 393
Loc: North Flag
Last seen: 1 year, 3 months
Always Helpful
04/04/17 05:43 PM

Man, I can tell you know your shit. Thats why I trust your suggestions. Great pics in the signature!
OfflineQjsands
Psyconaut
 User Gallery

Registered: 03/03/17
Posts: 181
Last seen: 5 years, 2 months
Always helpful
04/01/17 12:54 AM

Thanks for the in depth responses man, you are a big help to me and many others. Always respectful.
Offlinemicolatria
Male User Gallery

Registered: 12/03/13
Posts: 452
Loc: shroomery
Last seen: 16 days, 14 hours
punx?
03/30/17 04:58 PM

:crankey: attitude
InvisibleJvF
Fletcher Detcher

Registered: 02/13/14
Posts: 2,662
Loc: Chicago Flag
I havent given you one yet?
03/10/17 07:22 PM

Lets fix that!

The grow in your sig is awesome, always love seeing that canopy :grin:

:vibin:
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/24/16
Posts: 6,827
Loc: Interdimensional Bootcamp
+5
03/08/17 04:35 PM

:thumbup:
OfflineKenetic
Nam Sayin
 User Gallery

Registered: 08/25/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 3 years, 11 months
Awesome
03/01/17 05:21 AM

Contributions and pics!  You know what you're doing.
InvisibleThe Patient
 User Gallery

Registered: 03/06/13
Posts: 545
5
02/21/17 05:41 AM

Wow fantastic pics man, gave me some inspiration for a future project. :mushroom2:
Offlinegermanauslander
Forever Learner

Registered: 11/06/16
Posts: 100
Last seen: 4 years, 2 months
good advice
02/14/17 12:52 AM

Re: Re: Agar culture from spore prints

thanks man
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