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OfflineB H O

Registered: 03/17/13
Posts: 675
Last seen: 1 year, 1 month
Solid TC!
06/30/21 07:28 PM

Thanks for answering my questions via PM
I'm a teapot User Gallery

Registered: 08/17/20
Posts: 692
Loc: Fungistan
Super friendly and willing to help
06/28/21 11:05 AM

Appreciated the effort you gave helping me figure out my lid and filter woes. Really much appreciated and was a huge help, appreciate the generosity with your time and knowledge. :grin:

Registered: 10/14/18
Posts: 320
Really appreciated this.
05/07/20 06:48 AM


mushpunx said:
It's just to make sure the culture is clean - even if the germination plate looks clean oftentimes the myc has germinated over top or next to bacteria or mold colonies. So make a transfer to another plate and use that to inoculate your grains. Often I'll do two transfers to be safe.
Whenever you make a transfer, you are probably cutting out at least some of the genetics, but it doesn't matter. You are just looking for a clean MS culture to inoculate grains with.
In a culture germinated from spores there are hundreds to thousands of strains present. If I am isolating strains it typically takes me 8 to 10 transfers before I see obvious sectoring. Then once I take a transfer from each sector to a new plate, those cultures might still show some sectors and I might still have to make another transfer or two of each of the next plates until they become single sector isolated strains. At that point, I'll have 10 - 20 different strains, which I label, fruit and test for potency + growing factors. There might only be one or two good ones, if I'm lucky.
Strain isolation takes a lot of work and patience!
It makes more sense for us home cultivators to clone. That is still quite a bit of work (and eating a lot of mushrooms if you wanna test potency 😂), taking lots of clones and fruiting them out, searching for potent, high yielding fruiters.

OfflineMagic Mushpoon
 User Gallery

Registered: 03/24/19
Posts: 104
Last seen: 9 months, 12 days
Good Advice
04/06/20 12:55 PM

Super helpful advice and experiences in a bunch of different threads
Woodland Creature

Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 2 years, 9 months
03/02/19 09:07 AM

InvisibleJohnny Dont
500 Don'ts of Knife Safety

Registered: 04/24/14
Posts: 1,830
Helpful and polite
02/14/19 04:11 PM

Thanks for the advice
Second Rate Mycologist
Male User Gallery

Registered: 08/18/09
Posts: 2,194
Loc: Between a Rock and a Hard...
Last seen: 5 months, 21 days
Good info
02/12/19 05:21 PM

My nose dont work neither lol
Salty Sailor
 User Gallery

Registered: 02/20/14
Posts: 754
Loc: Norway Flag
Last seen: 2 years, 11 months
Peace of mind
02/08/19 05:41 PM

Male User Gallery

Registered: 12/30/16
Posts: 6,809
Loc: PNW
5 shrooms
01/31/19 08:47 PM

Nice seeing you around.  Always dropping good knowledge
Hi mom!

Registered: 05/01/16
Posts: 108
Loc: Night City 2020
Last seen: 4 years, 10 months
Thanks for the help!
01/21/19 08:47 AM

Thanks for helping with my flow hood questions!
The Goddess Kali Meh 😛
I'm a teapot User Gallery

Registered: 07/01/17
Posts: 6,067
10/16/18 05:59 PM

So glad to see you back in cult again!! You're one of the best on here!
Male User Gallery

Registered: 06/10/12
Posts: 430
Loc: New York
Last seen: 10 months, 16 days
Incredible patience, kudos!
08/11/18 09:52 PM

Stony Danza
Male User Gallery

Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
02/08/18 01:17 AM

5 more mush for da punx
Post whore
Male User Gallery

Registered: 02/17/17
Posts: 4,727
Loc: Flag
08/12/17 07:15 AM

Smart man. Good man.
foil hater

Registered: 07/26/15
Posts: 1,215
Loc: Colonial alley
Last seen: 2 days, 9 hours
08/05/17 11:51 AM

Where is your tc tag at? Thanks for the blower sizing for a 2x2 flow hood info in that one thread.
I'm a teapot User Gallery

Registered: 07/06/11
Posts: 888
Loc: Uranus
Shares info willingly
05/14/17 05:19 AM

I appreciate your help and info. Thank you
Male User Gallery

Registered: 09/12/16
Posts: 310
Loc: Canada Flag
Last seen: 1 year, 8 months
thanks man
04/09/17 08:47 PM

the strength is strong in this one.

real informative
The Fool on the Hill
Male User Gallery

Registered: 06/18/15
Posts: 393
Loc: North Flag
Last seen: 2 years, 8 months
Always Helpful
04/04/17 08:43 AM

Man, I can tell you know your shit. Thats why I trust your suggestions. Great pics in the signature!
 User Gallery

Registered: 03/02/17
Posts: 181
Last seen: 6 years, 7 months
Always helpful
03/31/17 03:54 PM

Thanks for the in depth responses man, you are a big help to me and many others. Always respectful.
Male User Gallery

Registered: 12/03/13
Posts: 465
Loc: Universo
Last seen: 2 days, 22 hours
03/30/17 07:58 AM

:crankey: attitude
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