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InvisibleHtaeh
Socially distanced, always
Male

Registered: 10/14/18
Posts: 279
Really appreciated this.
05/07/20 08:48 AM

Quote:

mushpunx said:
It's just to make sure the culture is clean - even if the germination plate looks clean oftentimes the myc has germinated over top or next to bacteria or mold colonies. So make a transfer to another plate and use that to inoculate your grains. Often I'll do two transfers to be safe.
Whenever you make a transfer, you are probably cutting out at least some of the genetics, but it doesn't matter. You are just looking for a clean MS culture to inoculate grains with.
In a culture germinated from spores there are hundreds to thousands of strains present. If I am isolating strains it typically takes me 8 to 10 transfers before I see obvious sectoring. Then once I take a transfer from each sector to a new plate, those cultures might still show some sectors and I might still have to make another transfer or two of each of the next plates until they become single sector isolated strains. At that point, I'll have 10 - 20 different strains, which I label, fruit and test for potency + growing factors. There might only be one or two good ones, if I'm lucky.
Strain isolation takes a lot of work and patience!
It makes more sense for us home cultivators to clone. That is still quite a bit of work (and eating a lot of mushrooms if you wanna test potency πŸ˜‚), taking lots of clones and fruiting them out, searching for potent, high yielding fruiters.


OfflineMagic Mushpoon
Stranger
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Registered: 03/24/19
Posts: 104
Last seen: 2 days, 15 hours
Good Advice
04/06/20 02:55 PM

Super helpful advice and experiences in a bunch of different threads
Offlinegizmodo
Woodland Creature
Male

Registered: 06/21/18
Posts: 2,034
Loc: Behind You
Last seen: 1 month, 27 days
+5
03/02/19 11:07 AM

:pipesmoke:
InvisibleJohnny Dont
500 Don'ts of Knife Safety

Registered: 04/24/14
Posts: 1,830
Helpful and polite
02/14/19 06:11 PM

Thanks for the advice
OfflineDionili
Second Rate Mycologist
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Registered: 08/18/09
Posts: 2,194
Loc: Between a Rock and a Hard...
Last seen: 1 year, 1 month
Good info
02/12/19 07:21 PM

My nose dont work neither lol
OfflineBlazeGlassDabs
Salty Sailor
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Registered: 02/20/14
Posts: 747
Loc: Norway Flag
Last seen: 1 year, 3 months
Peace of mind
02/08/19 07:41 PM

πŸ–’
InvisibleJust_A_Noob
Becoming
Male User Gallery

Registered: 12/30/16
Posts: 6,397
Loc: PNW
5 shrooms
01/31/19 10:47 PM

Nice seeing you around.  Always dropping good knowledge
OfflineJesh
Hi mom!
Male

Registered: 05/01/16
Posts: 108
Loc: Night City 2020
Last seen: 1 year, 4 months
Thanks for the help!
01/21/19 10:47 AM

Thanks for helping with my flow hood questions!
OfflineTormato
It’s OK!! I’m a Limo Driver!! 😁
Male User Gallery

Registered: 07/01/17
Posts: 5,368
Last seen: 14 hours, 20 minutes
+5
10/16/18 07:59 PM

So glad to see you back in cult again!! You're one of the best on here!
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
Offlineblthirteenre
Person
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Registered: 06/10/12
Posts: 427
Loc: New York
Last seen: 1 year, 23 days
Incredible patience, kudos!
08/11/18 11:52 PM

https://www.shroomery.org/forums/showflat.php/Number/25374586#25374586
InvisibleAyePlus
Tote-tally awesome
I'm a teapot User Gallery

Registered: 12/18/14
Posts: 1,997
Loc: PNW
5
02/08/18 03:17 AM

5 more mush for da punx
InvisibleJHOVA
Post whore
Male User Gallery

Registered: 02/17/17
Posts: 4,139
Loc: Flag
Knowledgeable
08/12/17 09:15 AM

Smart man. Good man.
Offlinewtfcrazymofo
Zym.zymo-fo
Other

Registered: 07/26/15
Posts: 1,135
Loc: City Vanity
Last seen: 10 days, 3 minutes
5
08/05/17 01:51 PM

Where is your tc tag at? Thanks for the blower sizing for a 2x2 flow hood info in that one thread.
Invisiblemistamonsta
Threadkiller
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Registered: 07/06/11
Posts: 665
Loc: Australia Flag
Shares info willingly
05/14/17 07:19 AM

I appreciate your help and info. Thank you
OfflineMartinsapin
no-mad
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Registered: 09/12/16
Posts: 225
Loc: Canada Flag
Last seen: 17 hours, 44 minutes
thanks man
04/09/17 10:47 PM

the strength is strong in this one.

real informative
OfflinePurpleHaz
The Fool on the Hill
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Registered: 06/18/15
Posts: 381
Loc: North Flag
Last seen: 4 months, 16 hours
Always Helpful
04/04/17 10:43 AM

Man, I can tell you know your shit. Thats why I trust your suggestions. Great pics in the signature!
OfflineQjsands
Psyconaut
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Registered: 03/02/17
Posts: 181
Last seen: 3 years, 1 month
Always helpful
03/31/17 05:54 PM

Thanks for the in depth responses man, you are a big help to me and many others. Always respectful.
Offlinemicolatria
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Registered: 12/03/13
Posts: 393
Last seen: 4 days, 13 hours
punx?
03/30/17 09:58 AM

:crankey: attitude
InvisibleJvF
Fletcher Detcher

Registered: 02/13/14
Posts: 2,662
Loc: Chicago Flag
I havent given you one yet?
03/10/17 12:22 PM

Lets fix that!

The grow in your sig is awesome, always love seeing that canopy :grin:

:vibin:
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 6,149
Loc: Interdimensional Bootcamp
+5
03/08/17 09:35 AM

:thumbup:
OfflineKenetic
Nam Sayin
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Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 1 year, 9 months
Awesome
02/28/17 10:21 PM

Contributions and pics!  You know what you're doing.
InvisibleThe Patient

Registered: 03/06/13
Posts: 527
5
02/20/17 10:41 PM

Wow fantastic pics man, gave me some inspiration for a future project. :mushroom2:
Offlinegermanauslander
Forever Learner

Registered: 11/06/16
Posts: 100
Last seen: 2 years, 1 month
good advice
02/13/17 05:52 PM

Re: Re: Agar culture from spore prints

thanks man
Invisibleb1blazin
The Transcendent

Registered: 06/17/12
Posts: 399
thnx
02/03/17 10:41 PM

good info on identifying sectors!
Offlinezathras
Weirdo loser
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Registered: 12/23/15
Posts: 124
Last seen: 2 years, 10 months
Those AA+ are sexy as hell
09/02/16 09:19 PM

You are killing it, love those AA+ genes you got going on. Jelly as fuck over here. Keep up the good work! you rule
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