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Psyconaught Reged: 07/10/07 Posts: 194 Loc: AB |
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I've been trying to find a way to create monokaryotic mycelium from dikaryotic mycelium obtained from a clone of the strangest looking cube I've ever seen. The goal being to then cross the resulting monokaryote with some particularly potent blue meanie cultures I have going.... So far I've come up with agar supplemented with 2-Deoxy-D-glucose at 5mM2 Or possibly performing mechanical dedikaryotization then growing out the fragments in a dextrose/glycine solution? Does anyone have any input or other techniques they use or know of? So far as I know these techniques haven't been tried on any species within this family.... -------------------- providing rare and exotic botanicals to the general public
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