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Offlinecheor
Psyconaught


Reged: 07/10/07
Posts: 194
Loc: AB
Dedikaryotization of Cubensis fruit body clones?
      01/15/22 10:17 AM

I've been trying to find a way to create monokaryotic mycelium from dikaryotic mycelium obtained from  a clone of the strangest looking cube I've ever seen.

The goal being to then cross the resulting monokaryote with some particularly potent blue meanie cultures I have going....

So far I've come up with agar supplemented with  2-Deoxy-D-glucose at 5mM2

Or possibly performing mechanical dedikaryotization then growing out the fragments in a dextrose/glycine solution?


Does anyone have any input or other techniques they use or know of?

So far as I know these techniques haven't been tried on any species within this family....

--------------------
providing rare and exotic botanicals to the general public

Post Extras Print Post Remind Me! Notify Moderator
. * * Re: Dedikaryotization of Cubensis fruit body clones? fossilshark   01/29/22 05:24 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? TheConfluence   02/08/22 09:54 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Alan Rockefeller   02/09/22 04:43 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? rhizoRider   02/10/22 08:10 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Nillion   04/14/22 05:36 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Psilotoad   04/23/22 02:08 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? QM33   04/25/22 06:33 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Psilotoad   05/17/22 09:43 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? QM33   05/17/22 12:12 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? QM33   05/17/22 12:21 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? *DELETED* Psilotoad   11/27/22 02:34 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Psilotoad   11/27/22 02:35 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Alan Rockefeller   11/28/22 04:52 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? DERRAYLD   11/28/22 11:09 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? cooleko   11/28/22 11:40 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Alan Rockefeller   11/30/22 02:36 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? AyePlus   01/21/23 09:45 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Alan Rockefeller   01/31/23 12:47 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? AyePlus   01/31/23 07:20 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? AyePlus   02/11/23 11:16 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Psilotoad   02/25/23 08:38 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? fahtsterT   02/26/23 01:00 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? wy35   02/28/23 12:11 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Nillion   12/27/23 04:01 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? AyePlus   03/04/23 11:25 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? tryptkaloids   08/17/23 06:41 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Mushroom love   08/18/23 01:01 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? trippleblack   08/20/23 07:59 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Mushroom love   08/20/23 12:06 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Zwinst   09/25/23 12:08 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? wy35   02/21/23 12:27 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Alan Rockefeller   02/23/23 10:57 PM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Mead   02/24/23 08:41 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Psilotoad   02/25/23 08:25 AM
. * * Re: Dedikaryotization of Cubensis fruit body clones? Nillion   12/27/23 03:56 PM


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