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Offlinerequiem99
Stranger


Reged: 10/12/05
Posts: 35
Tell me how bad I fucked my project up.
      01/31/06 03:56 PM

I'm following the PFTEK for simple minds as linked to on this site (currently 3 days into incubation and awaiting mycelial growth), with the following changes, all due to necessity and none to my own genius or "invention":

1) I did not use triple foil lids, I used single foil to sterilize the jars and then injected spores and immediately added sterile metal lids (no holes). Foil was sterlized prior to injecting with spores.

2) I sterlized using steam. I know this is iffy but I didn't have access to a pressure cooker OR a place to use it, so I boiled for 30-60 minutes, waited a day, then boiled again for 30-60 minutes a second time. The water ran out a few times and a few of the jars were subjected to dry heat, so this may have altered the water content -- I really don't know if it would since the jars were sealed. Someone can help me out on this one.

3) The spore syringes I used (1 syringer per 6 jars for 12 total) were PR and KS, not the best strains for a newbie but I didn't buy them when I knew what i was doing. That brings me to another thing: They've been kept in storage (a cool drawer sealed in the vacuum pack until 3 weeks ago) for 3 months since I bought them, and have been moved around in a bag to other locations 3 or 4 times. Does this mean I'm SOL and nothing will happen?

Also, do my jars need holes to incubate properly or are they fine as sealed?

I can post pics later on if anyone wants.

Post Extras Print Post Remind Me! Notify Moderator
. * * Re: Tell me how bad I fucked my project up. ChronicStiffy   01/31/06 04:33 PM
. * * Re: Tell me how bad I fucked my project up. Snaggletooth   01/31/06 04:35 PM
. * * Re: Tell me how bad I fucked my project up. Pr0_X   01/31/06 04:42 PM
. * * Re: Tell me how bad I fucked my project up. Hengman   01/31/06 04:43 PM
. * * Re: Tell me how bad I fucked my project up. *DELETED* Orranis   01/31/06 06:08 PM
. * * Re: Tell me how bad I fucked my project up. requiem99   01/31/06 07:32 PM
. * * Re: Tell me how bad I fucked my project up. requiem99   01/31/06 07:43 PM
. * * Re: Tell me how bad I fucked my project up. FooMan   01/31/06 07:56 PM
. * * Re: Tell me how bad I fucked my project up. requiem99   01/31/06 07:57 PM
. * * Re: Tell me how bad I fucked my project up. *DELETED* Orranis   01/31/06 09:31 PM
. * * Re: Tell me how bad I fucked my project up. Oatman2000   01/31/06 09:44 PM
. * * Re: Tell me how bad I fucked my project up. Triad   01/31/06 09:47 PM
. * * Re: Tell me how bad I fucked my project up. coda   01/31/06 10:24 PM
. * * Re: Tell me how bad I fucked my project up. requiem99   02/01/06 10:06 AM
. * * Re: Tell me how bad I fucked my project up. requiem99   02/01/06 07:26 PM
. * * Re: Tell me how bad I fucked my project up. Maverick   02/01/06 07:32 PM
. * * Re: Tell me how bad I fucked my project up. shroomsynergy   02/01/06 07:41 PM
. * * Re: Tell me how bad I fucked my project up. Hypercube   02/01/06 07:44 PM
. * * Re: Tell me how bad I fucked my project up. blueferret   02/01/06 07:56 PM
. * * Re: Tell me how bad I fucked my project up. requiem99   02/01/06 11:24 PM
. * * Re: Tell me how bad I fucked my project up. requiem99   02/03/06 09:35 PM
. * * Re: Tell me how bad I fucked my project up. shroomsynergy   02/04/06 12:20 PM


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