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Stranger Reged: 10/12/05 Posts: 35 |
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I'm following the PFTEK for simple minds as linked to on this site (currently 3 days into incubation and awaiting mycelial growth), with the following changes, all due to necessity and none to my own genius or "invention": 1) I did not use triple foil lids, I used single foil to sterilize the jars and then injected spores and immediately added sterile metal lids (no holes). Foil was sterlized prior to injecting with spores. 2) I sterlized using steam. I know this is iffy but I didn't have access to a pressure cooker OR a place to use it, so I boiled for 30-60 minutes, waited a day, then boiled again for 30-60 minutes a second time. The water ran out a few times and a few of the jars were subjected to dry heat, so this may have altered the water content -- I really don't know if it would since the jars were sealed. Someone can help me out on this one. 3) The spore syringes I used (1 syringer per 6 jars for 12 total) were PR and KS, not the best strains for a newbie but I didn't buy them when I knew what i was doing. That brings me to another thing: They've been kept in storage (a cool drawer sealed in the vacuum pack until 3 weeks ago) for 3 months since I bought them, and have been moved around in a bag to other locations 3 or 4 times. Does this mean I'm SOL and nothing will happen? Also, do my jars need holes to incubate properly or are they fine as sealed? I can post pics later on if anyone wants.
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