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InvisibleHippie3
mycotopiate


Reged: 11/06/99
Posts: 3090
Loc: mycotopia.net
Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags
      10/19/05 06:56 AM

hi,
yer old friend Hippie here.
Just wanted to share something that's catching on over at mycotopia.net

a simple way to convert an empty syringe into an effective filter for air
to equalize pressure in sealed jars/bags
neutralizing the partial vacuum formed by sterilization
or the removal of fluid from a liquid culture jar.
see http://forums.mycotopia.net/showthread.php?t=5245
for the original thread info.
here's some pix-






ok,
that's how to make the airport itself, just pc it once to sterilize the interior
and it will filter air that passes thru it thereafter.


now how to use it on a jar of spore water or a liquid culture-
the lid is prepared thusly-









one can use this even to inject nutes/spores/air into sealed bottles of distilled water, sport drinks, etc. as needed.
many possible uses.
also see
http://forums.mycotopia.net/showthread.php?t=5177
and
http://forums.mycotopia.net/showthread.php?t=5001
and
http://forums.mycotopia.net/showthread.php?t=5212


here's how to use the airport to bubble air into a liquid culture to increase
dissolved gases and boost growth.






Quote:

I made a lid. i put 4 holes in it then put silicone over holes on both sides and allowed to dry for 24 hours. I then made my syringes i took a bunch of polyfil and stuffed it into 2 syringes and packed it down to make the filters then i took 1 syringe and siliconed an air tube into it to automat the process instead of blowing on one end(it makes it easier when bubbling the solution later) and i let it sit for 48 hours to totally let it dry it wasn't totally dry after 24 hours.

I started my LC by using a 250ml jar, i put 100ml water in the jar and 1 tablespoon of vanilla extract. i then put the lid on the jar seal side down b/c i want the jar to have a tight seal but left the ring loose on the jar. PC at 15 P.S.I. for 30 min then after PC i released the pressure from my cooker by the release valve and while hot i tightened the ring to keep seal. let cool. i then took a cotton swab with alcohol on it and put it over the hole and pierced it with one of the syringes to kill the vacuum in jar(or else it will suck all your spore syringe into the jar). i then used the same cotton swab and pierce through the silicone with my spore syringe. and allowed to grow. every 4 days i would stick my 2 syringes in to the holes. i put the non airtube syringe in first then the airtube one tipped the jar on its side and turned the pump on. the reason i like the air pump is that you can watch it bubble through the solution but also make sure you don't blow any into the other syringe.







i'm sure y'all can figure out where to go from here.
enjoy.
peace & love
-hip

--------------------
Admin @ mycotopia.net
Mycotopia

Edited by Hippie3 (10/19/05 12:20 PM)

Post Extras Print Post Remind Me! Notify Moderator
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags ohmatic   10/19/05 07:49 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   10/19/05 07:52 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   10/19/05 08:10 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   10/19/05 08:17 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   10/19/05 09:01 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Roadkill   10/19/05 09:03 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags blackout   10/19/05 10:14 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags mskip23   10/19/05 10:21 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags backupwards   10/19/05 01:51 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   10/20/05 05:40 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   10/20/05 05:44 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Holydiver   10/20/05 06:37 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   10/20/05 07:11 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags agar   10/20/05 12:14 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   10/20/05 12:23 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags blackout   10/20/05 12:35 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags agar   10/20/05 01:30 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Olgualion   10/20/05 03:28 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   10/20/05 04:48 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   10/20/05 05:11 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags blackout   10/21/05 02:30 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   10/24/05 06:52 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Tweexican   10/24/05 07:17 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   10/24/05 08:09 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Tweexican   11/05/05 01:38 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   11/05/05 05:01 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Tweexican   11/05/05 11:29 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   11/05/05 11:38 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Tweexican   11/05/05 03:37 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   11/05/05 03:48 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   03/15/06 03:45 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Dragonaut   03/17/06 01:47 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   03/22/06 05:58 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags JSshroom   03/24/06 10:56 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   03/24/06 05:54 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   03/25/06 06:57 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Pinback   03/25/06 07:26 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   03/25/06 09:09 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Jaeger   03/25/06 10:56 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   03/26/06 07:32 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Tweexican   03/29/06 06:42 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   03/30/06 05:17 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   03/30/06 05:27 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags JSshroom   04/10/06 04:34 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Hippie3   04/10/06 04:37 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags agar   10/20/05 12:45 PM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags Tweexican   10/21/05 10:17 AM
. * * Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags agar   10/21/05 09:37 PM


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