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Reged: 11/21/04
Posts: 9056
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:LIQUID CULTURE - THE EASY WAY:
      05/06/05 09:52 AM



:LIQUID CULTURE - THE EASY WAY:

(how to turn 2ml/cc of spore solution into 20 ??..ten ml/cc syringes - right - cheap & easy.

Assuming you have an empty syringe & another with spore solution.

Go to Kmart, Target or any large discount Mart & buy a 3 pack of EvenFlow plastic baby bottles.

Also buy a small bottle of Karo corn syrup, a tube of silicone sealant & a few glass marbles.

All the baby bottle parts (bottle - screw cap - nipple) will withstand sterilization in a pressure cooker.

Remove the nipple, apply a small amount (1/8th to ? inch) of silicone sealant to the inside (interior) of the nipple, to serve as a self sealing syringe needle injection point. Allow the sealant to cure overnight

Note: the baby bottle has milliliter (ml) graduation marks up to 240 ml (8 fluid ounces).

Add 200 ml of water to the baby bottle (use marks on it - as a measuring point).

Take your empty syringe and aspirate 6 (six) ml of karo syrup into it.

Then inject that into the open baby bottle, resulting in an approximate 3% solution.

Next, add a glass marble to the baby bottle, to serve as an agitator devise - later.

Insert the nipple into the baby bottle (facing down - inside the baby bottle).

Attach the screw ring lid to hold the nipple in place over the mouth of the baby bottle.

:IMPORTANT:
Attach the screw ring loose, to allow steam to escape & pressure equalization during pressure cooking.

Pressure cook the baby bottle (or several) 20 minutes @ 15 psi.

:IMPORTANT:
Allow the pressure cooker to cool to room temperature, otherwise a vacuum can form inside the bottle, if the screw ring is tightened while the bottle is hot..

Open the cooled pressure cooker & immediately tighten the screw rings on the bottle (or bottles).

You now have a sterilized nutrient solution, in which to inject 2 or 3 ml/cc of spore solution into.

Once you inject a spore solution into it, simply incubate it around 78 to 82F in a clean environment.

You should be able to see tiny strands of mycelium (from germinating spores) in the solution within several days. Be patient, as some spores take longer to germinate - than others.

Once you have visible mycelium in the culture, gently agitate in on a daily basis.

Once the mycelium in the culture is fairly thick, it is ready to be aspirated into sterilized syringes, and used an inoculate.

~~~~~~~

FOR THOSE WITHOUT A PRESSURE COOKER:

NOTE: Not as fool/proof - fail/safe as pressure cooking sterilization.

Cut a piece of tyvek in a circle. Use a rubber band to hold it in place over the screw cap of the baby bottle.

Insert the baby bottle into your microwave (be sure the lid is somewhat LOOSE).

Then, microwave heat it until the solution in it - comes to a boil.

Then - stop, wait a moment & do the same thing again. Repeat, several times.

Remove the baby bottle (gently - as the lid is still loose) & allow to cool to room temperature.

Then, tighten lid.

~~~~~~~~~~~~~~


Or, if you want to do gallons, use jars - with filter lids.

--------------------


Post Extras Print Post Remind Me! Notify Moderator
. * * Re: :LIQUID CULTURE - THE EASY WAY: jtk   05/06/05 09:59 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Sammy   05/06/05 09:59 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Sammy   05/06/05 10:00 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Lucid_Euphoria   05/06/05 10:12 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: HippieChick   05/06/05 10:21 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/06/05 10:28 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Lucid_Euphoria   05/06/05 10:33 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: blackout   05/06/05 10:49 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/06/05 11:12 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Aeon01   05/06/05 11:54 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: scatmanrav   05/06/05 01:04 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: blackout   05/06/05 01:08 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/06/05 02:33 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: GnuBobo   05/06/05 02:36 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/06/05 02:48 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: GnuBobo   05/06/05 02:57 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: backupwards   05/06/05 03:36 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: backupwards   05/06/05 03:38 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: blackout   05/15/05 08:54 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Abermelin   05/15/05 09:36 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: vescudero0   05/15/05 11:30 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: kake   05/15/05 03:01 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: yodabuds   06/21/05 12:04 AM
. * * bling finding_self   06/21/05 02:39 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Wronguy   06/21/05 06:41 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: misterdogman   03/22/08 11:30 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Atheist   03/22/08 11:40 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: misterdogman   03/22/08 05:35 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Birthbytongue   05/28/06 02:14 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Blutjager   07/29/06 01:13 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: stormhalter   07/29/06 06:41 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: stormhalter   07/29/06 06:48 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: noleshroomer   08/04/06 01:13 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/06/05 03:42 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: backupwards   05/06/05 03:46 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: scatmanrav   05/06/05 04:41 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/06/05 06:53 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: lordoftheshroomz   05/06/05 08:14 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: MrMaddHatter   05/06/05 10:05 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: GnuBobo   05/06/05 10:16 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/06/05 11:29 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: captmueller   05/08/05 01:37 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* seatrip   05/06/05 10:44 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/06/05 11:31 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: GnuBobo   05/06/05 11:34 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: ScavengerType   03/22/08 06:16 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: blackout   05/06/05 01:06 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: HippieChick   05/06/05 10:00 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/06/05 10:07 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: X24   05/06/05 11:05 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: VALIS   05/07/05 12:49 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: HippieChick   05/07/05 12:55 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: VALIS   05/07/05 01:00 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/07/05 01:13 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: VALIS   05/07/05 01:24 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/07/05 02:00 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: @cro   05/09/05 03:02 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: scatmanrav   05/08/05 06:37 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Blue HelixT   05/08/05 09:21 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/09/05 07:01 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: IGnosticAbhorI   05/09/05 08:03 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/09/05 08:28 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Blue HelixT   05/09/05 07:09 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Silly_Cyben   05/10/05 12:17 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: down44   05/10/05 12:49 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   05/10/05 07:33 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: ticktock   05/11/05 08:32 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: lardnar   12/11/05 01:40 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: ke1n   01/19/06 12:44 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Shdwstr   01/19/06 01:28 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: joker_66599   01/27/06 08:57 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Nunbuh_Chrubble   01/27/06 10:36 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* cappa   04/11/06 04:07 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* Snaggletooth   04/11/06 04:16 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* joker_66599   05/27/06 04:16 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* Atheist   05/27/06 04:25 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Blutjager   01/16/07 08:15 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: davesj1   05/14/05 12:45 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: RootedGrowth   05/14/05 01:07 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: IGnosticAbhorI   05/14/05 01:12 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: CLUTCH   05/14/05 06:49 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* aaaa   10/16/05 08:05 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: mskip23   10/16/05 08:32 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: jarroddupont   10/16/05 08:40 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: jarroddupont   10/16/05 09:01 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: agar   10/16/05 09:22 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: jarroddupont   10/16/05 09:22 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Oatman2000   10/18/05 04:11 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: unknown28   10/18/05 04:30 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Fospher   10/18/05 04:50 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: etam   05/28/06 03:24 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Brainiac   05/28/06 03:47 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: etam   05/28/06 03:59 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Oatman2000   05/28/06 01:07 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Bamaman   09/24/06 08:49 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Bamaman   09/25/06 01:44 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: fufungi   09/26/06 12:01 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Dihnekis   09/26/06 12:31 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Omnicracker   09/26/06 02:55 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Lun4e   01/16/07 01:35 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: etam   01/16/07 02:05 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: Primate   01/16/07 08:11 AM
. * * Re: :LIQUID CULTURE - THE EASY WAY: OwMyHead   08/01/08 04:06 PM
. * * Re: :LIQUID CULTURE - THE EASY WAY: (moved) AnnoA   11/02/08 08:29 AM


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