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Reged: 08/04/12 Posts: 7623 Loc: Canada |
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Chitinases Quote: Quote: Characterization of a chitinase with antifungal activity Bacteriocins Quote: Bacteriocins from the rhizosphere microbiome https://www.ncbi.nlm.nih.gov/pm It's notable that many of the infected cultures did not exhibit any morphological traits and where white, some possesed rings, others fluffy mycelium with exudates. With all white-colored colonies having villous texture, these white cultures were less virulent and lacked the presence of conidia. So even under the scope we wouldn't likely be able to identify. This mentions one mentions inhibitory zones: "There was no zone of inhibition, found in this interaction." https://www.semanticscholar.org This seems like an odd mention. I'd like to find out more about culture morphology that includes zones of inhibition. "Chitinases of pathogenic fungi not only play vital roles in spore germination, septum formation, cell division, and morphogenesis, but the enzymes are also important in the host interaction . In addition to degradation of the host fungal cell wall, chitinases also inhibit hyphae growth and bud tube elongation. https://www.frontiersin.org/art I am convinced that scalloped edges are morphological identifiers for some inhibitory substance like chitinases or BLIS (bacteriocin-like inhibitory substances), I refuse to believe that inhibitory zones are healthy growth patterns. The left hand plate was used to produce the following: Front and back of the same plate . These plates are heavily scalloped, floccose and thicken toward the margin which prevents light from passing through the culture. Inhibitory zones are used to test the strength of both antibiotics and fungicides: Antifungal properties of Chitinases: https://www.researchgate.net/fi Considering that Mycogone, Aspergillus, Trichoderma, Bacteria, etc, can produce Chitinases; all bacteria can produce at least one form of bacteriocin. It seems reasonable to suspect that these compounds will be found on our plates and certainly found in grain processed for spawn production, furthermore it seems reasonable to suspect that these compounds if present would effect morphology by preventing growth of the host organism where these products are found. I believe that many of the positive visual identifiers for generally stressed grain masters may be the result of these compounds, or similar compounds; live vegetative bacteria may not be present but their byproducts may very well be. Chitinases, BLIS, or some similar mechanism seems reasonable, it also appears to be reasonable to state that symptom severity will reflect stage of infection, virulence etc. Edited by Stipe-n Cap (12/24/22 12:46 PM)
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