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I will abduct andprobe your anus Reged: 01/14/14 Posts: 6290 Loc: Star Wars Galaxy |
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Hello guys, I've been using these pp5 containers and I'm in love with them, I think they are the holy grail of no pour agar pp5 containers. Features:
The closest thing for a no-pour polypropylene container to the visibility of a petri dish and without having to lose money and time with parafilm ![]() I achieved very little/no condensation in most of the containers after sterilization, minimal or no pooling when following this tek. You will need 3 things for this tek. First of all the containers. A second source Third source (12$ for 20 BUT THEY HAVE DEFECTIVE LID UNFORTUNATELY) You will need this tall container or something similar! This one is a chocolate Christmas container. (11.25 inches tall - 3 inches diameter) And you will need a BIG sterilizer(not really, see the mason jar method in the suggestions bellow), I'm using a AA75X. I know for noobs this might be a big step back, but oh well, you can't have everything. You need a big sterilizer because the tall container is 11 inches tall.EDIT: Condensation or pooling will evaporate in aprox 2 weeks with the lid loose, see the suggestions page bellow). Pour the agar in the containers, i love it because 100 ml of liquid agar can pour 10 containers. So roughly 10 ml of agar per container, easy. (EDIT: I use now 20ml of agar per container, easier to do whole wedge transfers.) This is how deep it would look roughly 10ml of agar per container. Let them cool completely before screwing the lids (screw the lids slightly loose). Put the tall container upside down and put the holy grail containers inside the large container, they fit perfectly inside. This tall container in particular can hold in the inside up to 10 HG containers . I place the tall container inside the sterilizer, my sterilizer can hold up to 10 of these tall containers, so i can sterilize 100 HG containers in a single shot. Sterilize them at 17 psi for 1 hour (EDIT: since in this tek we sterilize the plates inside a mason jar or any other outer container, these plates seems to require more sterilization time to achieve the proper temperature inside the outer container, 1 hour appears to be enough). Turn off the sterilizer and walk away, do not release the steam valve, let it slowly go to 0 psi, when is at 0 psi start the counter for 1 hour, once the sterilizer is a little more cooler (still will burn your hand if you touch it), remove the lid of the PC. DON'T LEAVE the PC to cool all night! this will cause pooling in your HG containers! Take out the tall container and put it in a room with stable temperature, not your bedroom with air conditioner or heater causing big temp fluctuations! Remove the upside down part of the tall container and ensure to stack all HG containers vertically on top of each other correctly, this is essential, very similar like when you pour agar in front of the hood or SAB and the petri that is on top is the one that get most of the condensation but the plates that are bellow don't get any condensation. (You can do this in open air, don't worry about contams.) 13 plates stacked in this picture: Put the upside down part of the tall container back on Wait until they cool completely, come back and this should be the result: 1.- The HG container at the top with most of the condensation (most of the condensation will go away with time anyway). 2.- less condensation 3.- even less 4.- Almost none 5.- No condensation 6.- Nope 7.- 8.- 9.- Some pictures of pan cyan mycelium growing happily and with great visibility. I'll add more pictures later. GREAT visibility, i would say 8.5 from 10, comparable to the visibility of a real petri dish. Eatyualive took pictures with better lighting than what i posted above: Quote: Awesome visibility for a pp5 ![]() ![]() ![]() Cons.
Edited by Mr. Alien (12/03/20 03:23 PM)
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