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InvisiblemushboyT
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Reged: 04/24/05
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Making Liquid Culture
      05/30/19 08:27 AM

:stirthepot:MAKING LIQUID CULTURE:stirthepot:



LC should ONLY BE MADE from clean agar plates. this is a point of logic not a 'can it be done' statement.
for the sake of making the 10 millionth 'why did i fail?' thread, just take my word and DONT MAKE LCs WITH SPORES.





WHAT IS LC?

LC would be the growing culture inside a nutrient rich liquid. the liquid is technically called a broth. this is also the case with agar.
so if you see the word BROTH, it simply means the liquid you have prepared.
AGAR and LC are the same thing(broths). only agar..has agar:stoned:

WHY LC?
because mushrooms:shrug:

HOW TO MAKE THE BROTH
measure out desired amounts of water. i like doing ~250ml water per broth. you can make gallon LCs if you want:shrug:
i do smaller amounts because they colonize quicker and 250ml of LC can last a long time. also cuts back on how much media you expose while inoculating and so on..

INOCULATION TEKS
JOSEX BIOPSY POKE - mandatory reading:oldman:
WEDGE METHOD(tek TBA)

LETS MAKE THE BROTH

weigh out 1gram of LME(light malt extract..powder!) i like doing ~1g per ~250ml water.

add the powder first THEN add COLD water. this prevents clumping.

do NOT use hot water to make broths:nono: the hot water can cook the grain powder giving you lumps:facepalm:

shake it up good..

sometimes i stash the prepared broth in the fridge for awhile(after grain cycles run) but you dont have too.

cover with foil, back off the cap and pc 15min @ 15psi.

liquids sterilize easily. 30min+ can caramelize the sugars/wastes time.
i remove from the PC the moment psi hits zero and tighten the cap:thumbup:

other points
unused broths have long shelf lifes. ive let them sit for weeks and they are ready for inoculation when you are.
i use unmodified lids. i screw the lid on tight after inoculation. the culture is suspended in liquid and they dont need much GE anyway.

NUTE TYPES:
you can use many nute sources to make broths. as with agar, the type of nute will determine clarity:thumbup:

LIGHT MALT EXTRACT


BRF unfiltered


GRAIN WATER


ive really only made three types. light malt, brf and grain water.

LC DISCLAIMER
some people say lc is advanced or contamination prone ect.. IME this is not correct. however, LC can and will exploit any cracks in your cultivation armour.
grains prepped too wet? cultures not entirely clean? SAB technique not down?? youll grow nothing but green:sad:


real truth
honey/sugary LCs are so 1995
:begone:

Edited by mushboy (05/30/19 08:59 AM)

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