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Medicine Man Reged: 12/14/11 Posts: 2109 Loc: The Other Side |
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This is my first time working with Golden Teacher. It's a cubensis variety that I have always wanted to research. I have been holding on to two GT prints for a little while but I never started a culture because I only had two prints and I didn't want to make a syringe to find out if the print was or wasn't clean at the expense of my print being a dirty print that I lost due to contamination. So I wanted until I was comfortable with agar work. Once I had some experience with and was confident that I could successfully work with agar I grabbed one of my two Golden Teacher prints and inoculated an agar dish in my glove box. I transferred the original culture a total of three times. My final culture was not a mono culture, but once I was sure that I was working with a clean culture I inoculated a light malt extract liquid culture with a small wedge of agar in my glove box. I also transferred a small piece of agar to a new agar dish so that I would have something to fall back on in case this project goes bad. Since making the LC and making the fourth culture transfer I have inoculated a grain jar with a wedge from the dish which I will be using as a master grain jar to be used in my first ever grain to grain work. But that's enough about my master grain jar because that will be a new post altogether once that jar has reached 100% colonization. Now back to the LC that I made with a wedge from the third agar dish. I think Paul Stamets identifies each new transfer from the previous culture with a "P Value" in which each new transfer gets the next higher number in the sequence of the culture. If I am wrong about this and do not understand the P value system please chime in on this thread and explain to me how the P value works. I do not want to spread wrong information. Until then I will say that the agar dish that I am working with in this grow was dish P3. I allowed the P3 Golden Teacher LC to colonize. Once the mycelium in he LC had established itself I gave it a good shake to break up the mycelium and help speed up the colonization time. On 10-11-13 I inoculated a total of four quart jars which were filled with wheat berries with about 2cc of LC each. The jars were prepared just like RogerRabbit does in his Let's Grow Mushrooms video. As of 11-29-13 only one of the jars had reached full colonization. The other three were close behind but they still haven't reached full colonization. In this grow I am working with a tub half the size of the standard monotub. It only needs a total of eight quarts of spawn and substrate to fill the tub up to the four inch mark. I am not 100% sure which book it is in but I am pretty sure that in "The Mushroom Cultivator: A Practical Guide to Growing Mushrooms at Home" Paul Stamets say that 10 to 1 is a good working ratio for spawn to substrate. With this in mind I decided that one quart of spawn would work just fine with seven quarts of substrate. For this tub I went with the Damion's Elementary Coir Tek. Before I started the tek I turned my flowhood on in my grow room to help clean the air in my grow room. I let the substrate sit in the bucket for an hour then I gave it a good shake to mix everything up. After I shook the bucket I let it sit for another half hour. After letting the substrate to do it's thing for an hour and a half I dumped the substrate in the tub in front of my flowhood. I placed a meat thermometer in the tub to monitor the substrate temperature. I left the tub to sit in front of the running flowhood until the temperature of the substrate had dropped down to 100F. Once the temperature had dropped down to 100F. I broke up the colonized wheat in the grain jar and poured it in to the tub. I mixed it all up, put the lid on, labeled the tub with the strain, the date it was inoculated on, cracked a beer and lit a smoke. My fingers are crossed that all goes well and in about a month or so I will have plenty of fatty Golden Teacher shrooms to print and add to my print collection. Even if this tub ends up contaminated and doesn't make it to harvest I still have a clean agar culture to fall back on, and I still have three of the four LC jars colonizing. I also have the jar that I inoculated with culture P4. Either way I will get the prints I need 12-5-13: The tub as of 12-17-13 in the morning And here is some pin porn of the tub after I opened it on 12-17-13 12-20-13: Edited by Willy Wonka (12/07/14 11:00 AM)
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