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Unmodify everything Reged: 01/02/23 Posts: 260 Loc: underneath the water |
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Q1: if bacteria is present in the inoculant for an LC, is a higher or lower nutrient percentage preferred? If bacteria is present in the inoculant, don't use it for LC. Clean it on agar then send clean myc to LC. Q2.1: For SSLC, I do not want to have to deal with the vessel having to sit on a stir plate for the duration of growth, partly because I only have 1 stir plate and partly because I just don’t want to have to have something electronic running for 2 weeks+ straight—Mr. Mommy would be none to pleased. I do not mind the idea of having to shake the bottles 1-3x daily. Shaking is fine Q2.2: Would a 1% agar (telephone brand) be enough to increase thickness/viscosity without the possibility of it solidifying with daily shaking? If I end up doing .18% LME, should I also do 1.8% Agar? natedawgnow's SSLC https://www.shroomery.org/forum Q3: How far in advance can/should an LC nutrient solution be made prior to inoculation? Is there any reason I shouldn’t just make 6-8 media bottles of solution to have ready and sterilized whenever I decide to noc’ it—or is a sugary solution sitting around (at worst) asking for bacteria or at best evaporation that will lead to a more concentrated solution? You can make it in advance, it will stay sterile as long as nothing gets into the bottle -------------------- LAGM 2024
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