| Home | Community | Message Board |
|
You are not signed in. |

This site includes paid links. Please support our sponsors.
|
|
Myco N00b Reged: 08/21/23 Posts: 230 Loc: La Luna |
|
||
|
Alright this is long… SSLC solution questions: Trying my hand again at making master LCs. This time, instead of pint jars fitted with 2-port + gas exchange synthetic filter…I’m using 250ml media bottles that will be filled approximately 1/2 full (~125ml) and unmodified. So I can shake the hell out of them and not have to worry about contamination or ports/sfs popping off. I have heard great things about SSLC for faster colonization time and am curious to see for myself. As most of my [limited] experience is with regular LC, I am debating whether I want to do everything as SSLC or just a bottle or two to see if I like it? Since I just bought (10) 250ml media bottles, I was considering making ~6, but maybe that’s too much given that I don’t know if I like SSLC or want to stick with regular LC. If I do not plan to use all of it right away is there any reason to prepare so many master LCs or does the long shelf life lean into a “fuck it, why not” attitude? @baba_yaga said that once the myc in an LC has fully grown out it’ll stall. He additionally said LC can be stored indefinitely at room temp. Is this a higher risk of contamination rather than in the refrigerator? I also have not decided if I will be free pouring or drawing the solution into syringes. Since the SSLC is thicker than normal LC from the addition of agar, would it require a 16g syringe or larger?—I currently have 18g. I honestly don’t love the idea of free pouring as my brain can’t really handle not having consistent measurements and data—plus additional moisture freaks me out. I hate seeing condensation of wet grains. I had pretty good success with my first round of Master LCs at .18% LME, but have been told I can use as little as .1% — given that sslc is inherently cloudier than regular LC perhaps a lower nutrient content lending itself to a clearer solution where bacteria will be more visually obvious would be of benefit? Q1: if bacteria is present in the inoculant for an LC, is a higher or lower nutrient percentage preferred? Q2.1: For SSLC, I do not want to have to deal with the vessel having to sit on a stir plate for the duration of growth, partly because I only have 1 stir plate and partly because I just don’t want to have to have something electronic running for 2 weeks+ straight—Mr. Mommy would be none to pleased. I do not mind the idea of having to shake the bottles 1-3x daily. Editing to fix these numbers Q2.2: Would a .1% agar (telephone brand) be enough to increase thickness/viscosity without the possibility of it solidifying with daily shaking? If I end up doing .18% LME, should I also do .18% Agar? Q3: How far in advance can/should an LC nutrient solution be made prior to inoculation? Is there any reason I shouldn’t just make 6-8 media bottles of solution to have ready and sterilized whenever I decide to noc’ it—or is a sugary solution sitting around (at worst) asking for bacteria or at best evaporation that will lead to a more concentrated solution? I’m thinking before I make a BUNCH of bottles, I should probably first, figure out if I like sslc and then second, dial in my ideal nute and agar percentages before prepping more than 1-2 bottles… -------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 ABCD stands for Always Be Collecting Data “I don't know anything about anything, but I know enough about everything to know that you don't know what the fuck you're talking about” Edited by MushroomMommy (01/23/24 01:40 AM)
|
| Extra information | ||||
|
12 members, 138 guests and 39 web crawlers are browsing this forum.
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a |
Forum Permissions
You cannot start new topics You cannot reply to topics HTML is disabled BBCode is enabled |
Rating:
Thread views: 540833 |
||
|
|
||||

