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Stranger Reged: 12/21/23 Posts: 2 |
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Is it worth continuing to transfer these plates? This is my first effort at germinating spores on agar and my first time working with PE. I searched the forums but couldn't find pics/information that would allow me to confidently identify what I have. I'm prepared to be told that I'm clueless and that I've been playing with some other mould. I streaked two MEA plates with a PE swab and embedded the cotton in a third plate. I saw one site of germination on one of the streaked plates after 2 weeks. It's now 4 weeks and I haven't yet seen any more evidence of germination in any of the 3 plates. I'll try different, softer agar next time. Plate 1 shows the original site of germination (which was located on streak path). I made 4 transfers with a sterile inoculation loop. The dark speck at the top/top-left in the back-lit pic of plate 1 is white when top-lit. The growth in all plates looks white when lit from above. Plate 2 is one of the transfers and looks the weirdest with the pronounced spiky growth emanating from the agar chunk in the centre and the trail of mycelium running to the edge of the plate. I can't recall whether I dropped agar from the loop before depositing it in the centre but the underlying agar isn't scraped/dented so I'm guessing not. Some of the other plates show spiky growth from the transferred agar chunk too. All plates are wrapped with cling film, which I've used in the exact same way with no problem on regular cube plates when working with clones. Edited by MRFRY (12/22/23 03:26 PM)
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