| Home | Community | Message Board |
|
You are not signed in. |

This site includes paid links. Please support our sponsors.
|
|
oxo Reged: 11/27/22 Posts: 107 |
|
||
|
I'm restarting culture work from early this year. I did a run of hot-poured condiment cups a few weeks ago, at about 25% Trich contamination rate due to rusty technique, difficulty handling the cups in a sterile way, and possible lack of sterilization of the cups. I cleaned up some dishes from abandoned cultures, and am going to do another agar run or two. I'm planning to do primarily A2A transfers, MSS > agar, and flow hood testing. I'm thinking a low-nutrient batch and a water agar batch. I plan to hot-pour and use the PP5 and glass plates. I find it easier and cleaner for me to pour after sterilization than to pour before sterilization. I don't like the PP5 plates due to poor visibility and difficulty removing parafilm, but since I have a lot of them, I plan to use them. I'm thinking of using the PP5 plates primarily for MSS > agar and flowhood testing, and using the glass petris for later-stage culture work like transfers, so I have better visibility of the cultures before sending them to grain. I don't plan to use the 4oz Mason jars for agar. They are too deep for culture work for my comfort, so I plan to use them for BRF pucks. I'm trying to dial in my agar technique and quality. Does this all seem reasonable? Any other ideas? Tx.
|
| Extra information | ||||
|
15 members, 184 guests and 48 web crawlers are browsing this forum.
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a |
Forum Permissions
You cannot start new topics You cannot reply to topics HTML is disabled BBCode is enabled |
Rating:
Thread views: 540833 |
||
|
|
||||

