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Reged: 03/29/19 Posts: 1494 |
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So I had this problem or something similar. Here is what my problem was and what I did to fix it. I used both telephone and Living Jin btw. I use the Instant Pot to make agar. I was having pours where the first plates were almost totally water and the last plates were really hard and dense from each bottle. I also saw the occasional puck of agar at the bottom. I also had issues with clumping at the end which I put down to starting too cool even though I kept increasing my water bath temperature and getting too much condensation. I tried adding a stir bar, (okay, really just left a steel kabob stick in the container) while in the PC cycle to stir before pouring, but that was not effective. What I believe I figured out was that I was giving the agar too much time to settle out before or after the pc cycle. I was setting everything up the night before and using the timer function to start the cycle while at work and then the keep warm function to have it perfect when I got home from work the next day. When I changed to doing the pc cycle immediately after dissolving the dry ingredients in tepid water, and increased the cycle time to 50 minutes for 1200 to 1500 ml I started getting really nice plates. I do use the keep warm function as a water bath, but even so, if you let it go too long in the water bath, the agar and nutes in the liquid will begin to settle and you get increasing hardness and nute levels as you pour. Stirring at this point is not effective as the agar just doesn't truly re-dissolve at this temperature. Maybe that is something to look at if you are spreading out your prep and pour times. If not, then I also got nothin. I don't use media bottles any more, but the problem was occurring when I used them or these until I shortened my prep to pour time and increased my cycle times. -------------------- Don't Panic
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