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Sponge Brain Reged: 07/08/18 Posts: 202 Loc: Rocky Mountains |
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Yeah Verum, thank you. I will definitely test my pouring technique this weekend with a new batch of agar. I realize I should have said that I would be using the same syringe for the test. Such common sense to me but, well yeah, I will be more specific next time. *edit* I almost always review TEKs before I work. In this case, with a massive contamination problem, I reviewed SAB technique, agar making and agar pouring technique. Upon review I made 1 mistake I knew about, and several that I didn't. 1-I had a towel and no rack for my SAB bottom, the petries were way to close to the towel. 2-I was only flaming my loop after every plate during the transfer because I wasn't touching the syringe to the loop, stupid!, I should have been flaming both every time. 3-I was not streaking the plate correctly. This didn't contribute to contams, but might have created more situations with viable myc that could be transferred. 4-The mistake I knew about, not realizing how significant it was. I was trying to pour from the bottom of the stack up. At one point I lifted the base of the next plate, and didn't see it through the condensation, and poured onto a lid. I knew I messed up but didn't think about how that created a vector for every lower plate to essentially touch the towel. I should have called this a learning experience at that point but I tried to use the good plates from the pour. Kinda of embarassing but hopefully someone learns from it and avoids the same mistake.
-------------------- Edited by Ginge (08/18/18 08:08 PM)
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but hopefully someone learns from it and avoids the same mistake.
