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Sponge Brain Reged: 07/08/18 Posts: 202 Loc: Rocky Mountains |
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So I wanted to be more fancy and ordered some petrie dishes. When using pasty plates I lost 1/36 due to contams when nocing with MS syringes. And over the course of 60 more plates lost 2 others to contamination. Pretty good, I thought. So I am trying to start PE in the petries. I see contams and myc on most of the plates, 10/16. I used the technique of PCing my agar in a jar, then pouring the plates in two stacks of ten, starting from the bottom in my SAB. Left them in the SAB overnight and did the MS work in the morning. Used a homemade Bod's inoculation loop flamed before every plate. The contamination is so great I want to blame the syringe, but I think that may be vein as my success sample is pretty small. I was thinking of cooking up some pasty plates and nocing them with the syringe to try and test the theory. And after typing this as a question I feel like that would be a pretty good test, sometimes it helps just to type it out, but I will still post, maybe somebody has a similar thought. --------------------
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