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Sponge Brain Reged: 07/08/18 Posts: 202 Loc: Rocky Mountains |
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I made a labeling mistake! Argh! I have two ziplocs of pasty plates, one has been PC'd one has not, in the fridge. I didn't label because I had 7 pc'd and 6 not, easy to tell apart. Then I had a random contam and had to transfer, grabbed a PC'd plate and transfered. Now I need/want to do some transfers and I do not know which is which. My question is, is there anything wrong with PCing a pasty plate twice to ensure all of my plates have been PC'd? Also, how long do you leave a plate and wait for MS inoculation? I noc'd 16 plates with treasure coast spores from a vendor July 15. 8 of them have already been transferred, 8 of them show nothing. --------------------
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