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Say hello to my little friend Reged: 09/15/12 Posts: 37808 Loc: Canada |
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The reason is that if you are using media that contains insoluble media like potato flakes or grain water, or if it has high populations extra time is needed. Bodhi knows his shit but I feel that an extra 15 min is worth not having to order lab grade media or worry about having all your plates go bacterial in 4 days. I tried a run once with Grain water agar and potato flake agar for 25 min at 20 psi and had it all go bacterial 4 days later. For the sake of 20 min I lost 4 days and almost lost a culture as well. -------------------- Pastywhyte's Easy Writeups My Favorite Grow Logs Avoiding the dreaded facepalm in cultivation The worst threads all have the same subjects Q & A.M.U. ![]()
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