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blue collar underworld Reged: 07/19/13 Posts: 22008 Loc: sub-surface unse |
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![]() and what Frank said (we need a :whatFranksaid: smiley) the edges of agar plate are most prone to contam because they are closest to the outside environment (spores, mold, etc) so the closer the myc gets to that environment, the more likely to contam it can be. if u don't have slants (i do not...yet) i just put them in fridge when they r grown out to this size: that way, if a contam did enter near plates edge, u will be able to see it on the agar. if the myc grows to the edge, u may not b able to see it, and it will hitch a ride with the wedge u take into ur grain jar/plate/bag, etc. u don't need parafilm for the pasty tek, but i still wrap them in saran wrap/cling wrap before putting them in fridge, or u can use plastic bags. -------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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