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stranger than ever. Reged: 11/20/13 Posts: 1500 |
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i think there's a distinction between that and liquid culture since there's zero nutrition in the liquid medium, and grain never enters into it. like you said, in stasis. i looked for the thread you mentioned, leaf, but i couldn't find. i'll try again later on. (pasty, i feel it's marginally on topic, but if you disagree, please advise) what i did probably sounds dumb, but i like it and so far it has been successful. using dropper dram vials such as these, i filled them with distilled water. PC'd for some amount to time, i forget off hand, to sterilize them. took two of them, and a fluffy multispore plate. one at a time in still air, squirted a few droppers full of water into the plate, scraped around with the dropper and slurped it all back up. rinsed, repeated. based on the work cited in the article, it should/could/would (might) remain viable for years, if stored in reasonable conditions. then just put a drop on agar to grow out for master inoculation and bickity bam, the motherfucker is rubble. (i love that 'motherfucker' wasn't marked as a misspelling) one drop at time, should last for at least as long as i'll be into growing, which i feel will be quite long, indeed. and when i'm done, they'll make great gifts. of course, it's not worth the effort for the sake of multispore, but with isolates, i feel it shows real promise, without refrigeration, or worries about repeating the slant process every year or so. in retrospect, yeah this is a clear , but i'm not wasting the above typing... sorry, y'all. you would not believe what a geek i am.
-------------------- the j stands for jesus.
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, but i'm not wasting the above typing... sorry, y'all. 