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blue collar underworld Reged: 07/19/13 Posts: 22008 Loc: sub-surface unse |
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pasty and other who use this tek, thought i'd tell u what i tried tonight, chime in and either me out cause it was dumb, or me on cause it just may work!so with this tek, the only consistent draw back i notice from multiple users of it, and other no pour teks like it, is the condensation. this can be slightly remedied by letting agar cool after pouring, before PC'ing. this cycle, mine cooled over 9 hours before pc'ing, there was NO moisture, condensation on walls or lids of the PP5. after pc cycle, i let it cool for over 12 hours. cool to the touch, not even warm. took them out, CONDENSATION everywhere WTF!! this does not happen all the time, but this time i thought for sure because i waited before pc and after pc cool down way more than i usually do that there would be no condensation. so what i did, while working in the SAB doing transfers, is after i take the foil off, i use the paper towel on top of lid (also sterile at this point) and wiped the walls of the container before dropping wedges in. obviously, if u do a cycle with a lot of these plates, u may not use ALL of them right outta the pc, so those put aside for a later date, will probably not still have a convenient sterilized paper towel to use for this (or do u think it would still b sterile, as it was wrapped in foil entire time?). i will let u guys know if it works. whatd'ya think? u can call me crazy, i don't mind. i did this once with a cotton ball (not even sterile, straight outta the bag) and a regular petri and it worked a while back (but it was one time, one plate, not conclusive enough), so i figured why not try it again with these because i use this tek a lot. -------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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me out cause it was dumb, or
me on cause it just may work!