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Say hello to my little friend Reged: 09/15/12 Posts: 37808 Loc: Canada |
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To inoculate with ms solution I like to first heat my loop red hot and then use it to melt a little divot into the surface of the agar. Then use a single drop right into the divot. This will help keep it from sliding around the plate. Leave it in the SAB for a day or two which will absorb the water into the agar, leaving the spores in one spot. This helps keep any bacteria confined and will make it easier to transfer away from. With a print, flame your loop or scalpel, then cool it in the agar. Scrape up a tiny amount of spores, then drag the loop or tip of scalpel across the surface. You could also hold the print above the plate and flick the spores onto the plate. This can be a little risky for introducing contams but does allow you to identify faster genetics. Ultimately you will need to experiment a bit and decide what works for you
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